Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,157,2710TG41.8% 86.2 / 13.3 55G165G (GGT→GGGycfHputative DNase
Reads supporting (aligned to +/- strand):  ref base T (9/23);  new base G (23/0);  total (32/23)
Fisher's exact test for biased strand distribution p-value = 1.98e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCATCG  >  NC_000913/1157125‑1157420
                                                                                                                                                  |                                                                                                                                                         
atCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTaaa                                                                                                                                                        >  2:346861/1‑150 (MQ=255)
  ccAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAAtt                                                                                                                                                      <  2:436974/150‑1 (MQ=255)
  ccAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTa                                                                                                                                                     <  2:517459/151‑1 (MQ=255)
   cAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTaaaat                                                                                                                                                      >  2:140058/1‑147 (MQ=255)
   cAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAtt                                                                                                                                                      >  1:467298/1‑149 (MQ=255)
      aTTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTgg                                                                                                                                                 <  2:32087/151‑1 (MQ=255)
        tGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAATTACTGGAt                                                                                                                                               >  2:396080/1‑151 (MQ=255)
         ggTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGGAATTatt                                                                                                                                                   >  2:146178/1‑144 (MQ=255)
            cGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAATTACTTGGtct                                                                                                                                            >  1:843127/1‑149 (MQ=255)
            cGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATaattggtatcg                                                                                                                                           >  1:245687/1‑141 (MQ=255)
                    gaacAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTa                                                                                                                                   <  1:132380/150‑1 (MQ=255)
                       cAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAATTATTgggttttggggttt                                                                                                                                     >  1:450387/1‑134 (MQ=255)
                           ccGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCt                                                                                                                         <  2:401905/151‑1 (MQ=255)
                                 aTCGTGCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGAGTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTcc                                                                                                                    <  1:583726/150‑1 (MQ=255)
                                 aTCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTcc                                                                                                                    <  1:815330/150‑1 (MQ=255)
                                    gTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAATTTCTTGATCTCGGGTTTTAAATATCCtttt                                                                                                                      >  1:781326/1‑145 (MQ=255)
                                    gTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATAATGGATCTCGGGTTTTTCATCTTCtttt                                                                                                                      >  1:937818/1‑145 (MQ=255)
                                      ccATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCAt                                                                                                               <  1:187030/150‑1 (MQ=255)
                                       cATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATtg                                                                                                             <  2:686840/151‑1 (MQ=255)
                                       cATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATAATTGATCTTGGATTTTATATCTTCTTTTTCGGCAtt                                                                                                              >  1:402813/1‑150 (MQ=255)
                                        aTACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGGAAATACTGGATCTCGGGATTTTCATCTTCtttttctggctt                                                                                                              >  1:567693/1‑141 (MQ=255)
                                        aTACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATTCTTGGTTTCGGGTTTTAAATTTCCTTTTTCGGCAtt                                                                                                              >  1:1022864/1‑149 (MQ=255)
                                            ccGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATACTGGGTCTCGGGTTTTAAAACCCCCTTTTCGGCATTTTGaac                                                                                                        >  2:535481/1‑149 (MQ=255)
                                                gACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGGTTTTACATCTCCTTTTTCCGCATTGTGACCTTcc                                                                                                    >  2:373624/1‑151 (MQ=255)
                                                 aCGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCCCGGATTTTACCTCTCCTTTTCCGGCATTGTGCCCTAcc                                                                                                    <  1:522726/150‑1 (MQ=255)
                                                   gCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGt                                                                                                  <  2:162590/150‑1 (MQ=255)
                                                         gCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACATAGGACAGAGAAACGGCGGGGAAAATTATGGGTGTTGGGTTTTAAATCCCCCTTTTCGGGCATTTGGCCCTCCGCAATGCgg                                                                                           >  2:118080/1‑151 (MQ=255)
                                                          ccGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAATTACTGGATTTCGGATTTTAAATCTCCTTTTTCGGCATTGTGACCTTCCGTAATGCgg                                                                                           >  1:389485/1‑150 (MQ=255)
                                                             aTACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATACTGGGTCTCGGGTTTTACATCTCCTTTTTCCGCCTTTTGACCTTTCGTAATGCgggg                                                                                         >  2:564719/1‑147 (MQ=255)
                                                              tACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAATTACTGGATTTCGGGTTTTAAATCTCCTTTTTCCGCCTTTTGACCTTCCGTAAAGCgggg                                                                                         >  2:605517/1‑146 (MQ=255)
                                                                   tGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAATTACTGGGTCTCGGGTTTTAAATTTCCTTTTTCGGCCTTTTGACCCTCCGTAAAGCgggg                                                                                         >  2:893690/1‑141 (MQ=255)
                                                                    ggCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTgc                                                                                  <  1:727588/149‑1 (MQ=255)
                                                                               gcgAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAAATAATGGATCTCGGATTTTAAATCTTCTTTTTCGGCATTTTGAGCTTCCGTAAATCGGGGGAAATGCGCGATGCTGcgcg                                                                     >  2:512858/1‑151 (MQ=255)
                                                                                     aaaaaGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGGAATTAATGGATCTCGGGTTTTAAATCTCCTTTTCCGGCCCTGTTACCTTTCCTAATGCGGAGGAAATGCGCCGTGCTGcgcg                                                                     >  1:904913/1‑145 (MQ=255)
                                                                                            gACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCt                                                         <  2:1023716/150‑1 (MQ=255)
                                                                                            gACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTg                                                       <  2:593513/151‑1 (MQ=255)
                                                                                               ggATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGAt                                                    <  1:89096/151‑1 (MQ=255)
                                                                                                 aTTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCg                                                  <  2:345468/151‑1 (MQ=255)
                                                                                                 aTTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAAATAATGGGTCTCGGAGTTTACATCTCCTTTTTCGGGATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCg                                                  >  2:31768/1‑151 (MQ=255)
                                                                                                   tGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATACTGGATCTCGGGTTTTAAATCTCCTTTTTCCGGCTTGTGGCCTTCCGTAATGCGGAGCAAATGCGCGCTGCTGCGCGTTTTTTTCCCCTGGAGCgg                                                 >  1:75199/1‑150 (MQ=255)
                                                                                                    gCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATACTGGATCTCCGCTTTTAAATCTCCCTTTTCGGCCATGTGTCCTTCCCTAATGCGGGGCAACTGCGCGGTGCTTCGCGTTTTGTTCCCCCGGGTCgg                                                 >  1:611528/1‑149 (MQ=255)
                                                                                                           cGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACtggtg                                        <  2:47920/151‑1 (MQ=255)
                                                                                                                tacACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAATTTATGGGTCTTGGGATTTTAATCTCCTTTTTCGGCGTTTTGATCTTCCCTAAATCGGTGGAAACGCGCGATGCTTTGCGTTATGTTCCCCCCGATCGGGTTTtggtggg                                      >  1:137704/1‑147 (MQ=255)
                                                                                                                 acacTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACt                                  <  1:970913/151‑1 (MQ=255)
                                                                                                                  cacTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTg                                 <  2:798548/151‑1 (MQ=255)
                                                                                                                        tttACAGAGGACAGAGAAACGGCGGGGAAATTACTGGATCTCGGATTTTAAATTTCCTTTTTCGGCCTTGTGACCTTCCGTAAAGCGGAGCAACTGCGCGGTGCTGCGCGGTATTTCCCCCCGGGTCGGTTACTGGGGGGAACTGACTCac                           >  2:71741/1‑151 (MQ=255)
                                                                                                                          tACAGAGGACAGAGAAACGGCGGGTAAATTACTGGGTCTCGGGTTTTTCATCTCCTTTTTCGGCATTGTGACCTTTCCTAATTCGGAGCAACTGCGCGATGCCGCCCGTTTTTTTCCCCCGGGTTGGTTAATGGGGGGAAATGACTCaac                          >  2:397068/1‑148 (MQ=255)
                                                                                                                          tACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCacc                          <  1:192115/150‑1 (MQ=255)
                                                                                                                          tACAGAGGACAGAGAAACGGCGGGTAAATTAATGGGTTTCGGGTTTTATATCTCCTTTTTCGGCATTGTGACCTTCCGTTATTCGGAGGAACTGCGCGATGCTGCGCGTTTTTTTCCCCCGGATCGGGTACTGGGGGAAACTGACCCacc                          >  1:118886/1‑150 (MQ=255)
                                                                                                                            cAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCacctta                       <  2:129715/151‑1 (MQ=255)
                                                                                                                             agagGACAGAGAAACGGCGGGGAAAATACTTGGTCTCGGGTTTTTAATATCCCTTTTCCGCACTGTGAACTTTCCTAATGCGGGGCAACAGCGCGGTGCTGCGCGTTTTGTTCCCCTCGGTTGGGTTCTTGGGGGAAACGAATCacc                          >  1:791370/1‑147 (MQ=255)
                                                                                                                                   cAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTgcg                <  1:595920/151‑1 (MQ=255)
                                                                                                                                             gcggGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGc      >  2:547164/1‑151 (MQ=255)
                                                                                                                                             gcggGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGc      >  2:759446/1‑151 (MQ=255)
                                                                                                                                              cggGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCa     <  2:245690/151‑1 (MQ=255)
                                                                                                                                                  tAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCATCg  >  1:414871/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                         
ATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCATCG  >  NC_000913/1157125‑1157420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: