Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,487,1630CT42.6% 58.5 / 34.0 54intergenic (+200/‑72)hrpA/ydcFputative ATP‑dependent helicase/DUF218 superfamily protein, SAM‑binding
Reads supporting (aligned to +/- strand):  ref base C (5/26);  new base T (23/0);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 9.29e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGTTATCGGACAGTGGCTGGCGCAGG  >  NC_000913/1487021‑1487313
                                                                                                                                                |                                                                                                                                                           
attaATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTAAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAAAAAATAAGAATttctcc                                                                                                                                                         >  2:8471/1‑147 (MQ=255)
  taATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCa                                                                                                                                                    <  2:21897/150‑1 (MQ=255)
    aTTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCagag                                                                                                                                                 <  1:128841/151‑1 (MQ=255)
      tCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAAGAAAAACGAATTTCTCCtttttgag                                                                                                                                                 >  2:222659/1‑144 (MQ=255)
         aaaaCGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTg                                                                                                                                               <  2:688816/148‑1 (MQ=255)
          aaaCGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTcc                                                                                                                                           <  1:802520/151‑1 (MQ=255)
             cGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAAGAAAAAAGATTTTCTCCTTTCTTAGGGCTcc                                                                                                                                           >  2:939115/1‑148 (MQ=255)
              ggTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAAGACATACAATTTCTTTCTTTTCTAGTGCt                                                                                                                                             >  1:417692/1‑145 (MQ=255)
                      tAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGAAAAACGATTTTCTCCTTTTAGAGTGCTCCGCCTCTCACtat                                                                                                                               >  2:209686/1‑151 (MQ=255)
                        aaaaTTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGATAAAGACAAAAGATTTTCTCCCTTTAGAGGGGTCCGCTTCTCTCtttt                                                                                                                              >  1:953595/1‑150 (MQ=255)
                          aaTTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAAGAAAAAAAAATTTCTCCTTTTTTAGTGCTCCCCCTCTCCCtattat                                                                                                                           >  1:567736/1‑150 (MQ=255)
                                 gAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCa                                                                                                                   <  2:41624/151‑1 (MQ=255)
                                   aTTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAAGACAAAAGAATTTCTCCCTTTCGAGGGCTCCCCCTCTCACtattttttccccc                                                                                                                     >  2:46654/1‑139 (MQ=255)
                                     tCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTa                                                                                                                <  1:350035/150‑1 (MQ=255)
                                      cAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAAGAAATACAAATTTCTTCCTTTTTAGGGCTTCGCTCTTCTCTATTATTTCACTCAGtttt                                                                                                             >  1:166602/1‑151 (MQ=255)
                                          aaaaTACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTa                                                                                                        <  1:498749/151‑1 (MQ=255)
                                                cAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGaa                                                                                                  <  1:704284/151‑1 (MQ=255)
                                                   ttCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAAGAAAAAAGAATTTCTCCTTTTTTAGAGCTCCCCCTCTCCCTATTTTTTCACGCCGTATTTTTAAagg                                                                                                    >  2:571184/1‑146 (MQ=255)
                                                    tctcTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAAGAAAAAACAATTTTTTCTTTTTGAGGGCGTCCCTTTTCACTATTTTCTCACCCAGTATTTTTAAaggga                                                                                                  >  2:606232/1‑145 (MQ=255)
                                                       ctgctgCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGAt                                                                                              <  1:545165/148‑1 (MQ=255)
                                                         gctgcAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAGGACATACGATTTTCTCCTTTTCGAGGGGTCCCCCTCTCCCTCTTTTTTTCCCCCGGATTCTTAAAGGAACGATaa                                                                                            >  2:715272/1‑148 (MQ=255)
                                                          ctgcAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAAGAAAAACAATTTTCTTCTTTTAGAGAGGTCCCCCTTTCCCTATTTTCTCCCGCAGGATTTTTTAAGGGAAGATaa                                                                                            >  2:132063/1‑147 (MQ=255)
                                                            gcAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAaggag                                                                                        <  1:503863/149‑1 (MQ=255)
                                                             cAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGaa                                                                                     <  1:835052/151‑1 (MQ=255)
                                                             cAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGAAATAAAAATTTTTTCTTTTAGAGGGGTCCGCTTCTCACTATTTTTTTACGCAGGATTTTTTAGGGGAAGAAAaggagg                                                                                       >  1:226682/1‑149 (MQ=255)
                                                              aaGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAAc                                                                                    <  1:604815/151‑1 (MQ=255)
                                                                  tGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAAGAGAAAAGATTTTTTCCCTTCAGAGGGCTTCCCCCCTCCCTATTATCTCACCCCGTATTCTTTAAGGGAAGGTAAAggggga                                                                                      >  2:597808/1‑146 (MQ=255)
                                                                   gAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATg                                                                               <  2:380700/150‑1 (MQ=255)
                                                                    aataatGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGaa                                                                             <  2:713019/151‑1 (MQ=255)
                                                                      taatGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGCTAAAGGAATAAAATTTTTTCCTTTTTGAGTGGTCCGCGTCCTCCTCTTTTTTTTCGCCGAATTCTTTAGGGGAACATAAAGAGGAAACATGaaa                                                                            >  1:733739/1‑149 (MQ=255)
                                                                       aatGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACa                                                                           <  1:740323/150‑1 (MQ=255)
                                                                        atGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAAGACAAAAAAATTTCTCCTTTCTTAGTGGTCCGCCTCTCACTATTTTTTCACCCCGGATTCTTTAAGGGACGATAAAGAGGGAACCTGAACa                                                                           >  2:730707/1‑149 (MQ=255)
                                                                          gTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTAc                                                                      <  2:81036/151‑1 (MQ=255)
                                                                           tttATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAAGAAAAAAGATTTTCTCCTTTTAGAGTGCTCCCCCTCTCCCTATTTTCTCCCCCAGTATTCTTTAAGGGAAGAGAAGGAGGaaaaacgaaaa                                                                            >  2:900862/1‑137 (MQ=255)
                                                                            ttATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTAcc                                                                     <  1:358154/150‑1 (MQ=255)
                                                                                 tACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGCTTAAGAAAAAAGATTTTCTCCTTTTTGAGTGGTTCCCTTTTCTCTATTATTTCTCTCAGTATTTTTTAAGGGAAGATTAAGAGGAAGCATTAAACTTAcccccctt                                                               >  2:42526/1‑147 (MQ=255)
                                                                                    aGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAGGAAATAAAAATTTTTTCTTTCTGAGTGGTCCCCTTTTTCATATTTTTTCAACCCGGATTTTTTTGGGGAAGATAAAGGGGGAACATGAAAAATACCCCGctt                                                               >  1:244879/1‑148 (MQ=255)
                                                                                    aGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGCTTAAGACAAAAGCTTTTCCTCCTTCTGGAGGTTCCGCTTTTCCCCATTCTTCTCCGCCGGTTACTTTAAGGGAAGGTAAGGGAGGACCATGAAAAATACCCCCTTTcc                                                             >  2:150062/1‑150 (MQ=255)
                                                                                      cATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAAGAAAAAAGATTTTCTCCTTTTTGAGTGGTCCCCCTCTCACTATTTTTTCACCCCGGATTTTTTAAGGGAAGAAAAAGAGGAAAAATTAAAAATAACCCCTTTTCCgc                                                           >  2:932350/1‑150 (MQ=255)
                                                                                         ttCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAAGACAAAAGATTTTCCCCCTTCTTAGGGGCCCCCTTCTCCCCATTTTTTTCCCCCGTATTTTTTAAGGGAAGATAAGGAGGAAACATTAACATTACCCCCGTTTc                                                              >  2:58319/1‑145 (MQ=255)
                                                                                              tAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTc                                                    <  1:252305/149‑1 (MQ=255)
                                                                                                   gATATGTCAGGCTTGCGGAGTGGCGGTTTAAGAAATAACATTTTCTCCCTTCAGAGTGCTCCCCTTCTCACTTTTATTTCCCCCAGAATTCTTAAAGGAACGATAAAGAGGAAACAAGAAAAATAACCCCCTTCCGACGCgttttcc                                                 >  2:583571/1‑143 (MQ=255)
                                                                                                   gATATGTCAGGCTTGCGGAGTGGCGGTTTAAGAAAAAAGGTTTTCTCCCTTTAAAATGGTCCGCTTTTCACTATTTTTTCACCCCGGATTTTTTAAGGGAAGATAAAGAGGGGACAACAAAAATAACCCGTTTTCGACCCGTTTGCCgg                                               >  2:722400/1‑149 (MQ=255)
                                                                                                    atatGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCaa                                            <  2:315300/151‑1 (MQ=255)
                                                                                                       tGTCAGGCTTGCGGAGTGGCGGTTAAGGAAAAACGATTTTCTCCTTTTTGAGGGCTCCCCCTCTCACTATTTTCTCACCCCGGATTCTTAAAGGAAAGATAAAGAGGGAACATGAACATTACCCCGCTTCCGACGCTTTCGCCCGCAAc                                           >  2:241752/1‑149 (MQ=255)
                                                                                                           aGGCTTGCGGAGTGGCGGTTTAGGAAATAAAATTTTCTCCTTTCTGAGTGCCCCCCTTCTCTCTCTTTTCTCACCCAGTATTTTTTAAGGGAAGAAAAAGAGGGAACATGAACATTACCCCGCTTCCTACGCtct                                                     >  1:123585/1‑133 (MQ=255)
                                                                                                            ggCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGAt                                    <  2:835599/151‑1 (MQ=255)
                                                                                                                    gAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATaaa                            <  1:830421/151‑1 (MQ=255)
                                                                                                                        ggCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGtt                        <  2:16362/151‑1 (MQ=255)
                                                                                                                        ggCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGt                         <  2:211120/150‑1 (MQ=255)
                                                                                                                           ggTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGTTATc                     <  2:400617/151‑1 (MQ=255)
                                                                                                                             ttAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTAGCCGGCAACTATAGATGCCATAAATGTTATCg                    <  2:328856/150‑1 (MQ=255)
                                                                                                                               aaGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGTTATCGGAc                 >  2:153461/1‑151 (MQ=255)
                                                                                                                                  gACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGTTATCGGACAGt              <  1:950746/151‑1 (MQ=255)
                                                                                                                                   aCATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGTTATCGGACAGtg             >  1:379919/1‑151 (MQ=255)
                                                                                                                                               tcctccTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGTTATCGGACAGTGGCTGgcgc     >  1:880483/1‑149 (MQ=255)
                                                                                                                                                cctcctTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGTTATCGGACAGTGGCTGGCGCAgg  <  2:569148/151‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                           
ATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGTTATCGGACAGTGGCTGGCGCAGG  >  NC_000913/1487021‑1487313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: