Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,713,302	G→T	R136L (CGT→CTT)	srmB →	ATP‑dependent RNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,713,302	0	G	T	100.0%	207.8 / NA	54	R136L (CGT→CTT)	srmB	ATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (24/30);  total (24/30)

GAGCTGGCGATGCAGGTGTCCGATCATGCCCGCGAACTGGCGAAACATACGCATCTGGATATCGCCACCATCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCT  >  NC_000913/2713154‑2713449                                                                                                                                                     |                                                                                                                                                    gAGCTGGCGATGCAGGTGTCCGATCATGCCCGCGAACTGGCGAAACATACGCATCTGGATATCGCCACCATCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTc                                                                                                                                                   >  2:13151/1‑151 (MQ=255)  aGCTGGCGATGCAGGTGTCCGATCATGCCCGCGAACTGGCGAAACATACGCATCTGGATATCGCCACCATCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTct                                                                                                                                                  >  1:589141/1‑151 (MQ=255)   gCTGGCGATGCAGGTGTCCGATCATGCCCGCGAACTGGCGAAACATACGCATCTGGATATCGCCACCATCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTctg                                                                                                                                                 <  1:492269/151‑1 (MQ=255)     tGGCGATGCAGGTGTCCGATCATGCCCGCGAACTGGCGAAACATACGCATCTGGATATCGCCACCATCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTctgc                                                                                                                                                >  1:116997/1‑150 (MQ=255)              aGGTGTCCGATCATGCCCGCGAACTGGCGAAACATACGCATCTGGATATCGCCACCATCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACAt                                                                                                                                      >  2:386658/1‑151 (MQ=255)              aGGTGTCCGATCATGCCCGCGAACTGGCGAAACATACGCATCTGGATATCGCCACCATCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACAt                                                                                                                                      >  1:541966/1‑151 (MQ=255)                 tgtCCGATCATGCCCGCGAACTGGCGAAACATACGCATCTGGATATCGCCACCATCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATaa                                                                                                                                    >  1:821731/1‑150 (MQ=255)                    ccGATCATGCCCGCGAACTGGCGAAACATACGCATCTGGATATCGCCACCATCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAaaga                                                                                                                                <  2:422409/151‑1 (MQ=255)                      gATCATGCCCGCGAACTGGCGAAACATACGCATCTGGATATCGCCACCATCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAaaga                                                                                                                                >  1:580821/1‑149 (MQ=255)                       aTCATGCCCGCGAACTGGCGAAACATACGCATCTGGATATCGCCACCATCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAagaaga                                                                                                                             <  2:420093/151‑1 (MQ=255)                              ccGCGAACTGGCGAAACATACGCATCTGGATATCGCCACCATCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATGAAAGAAGAGAACtt                                                                                                                       <  1:269003/150‑1 (MQ=255)                                 cgAACTGGCGAAACATACGCATCTGGATATCGCCACCATCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGAt                                                                                                                   <  2:588855/151‑1 (MQ=255)                                 cgAACTGGCGAAACATACGCATCTGGATATCGCCACCATCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGAt                                                                                                                   <  1:296982/151‑1 (MQ=255)                                       ggCGAAACATACGCATCTGGATATCGCCACCATCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCcg                                                                                                              <  2:132760/150‑1 (MQ=255)                                             aCATACGCATCTGGATATCGCCACCTTCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGtt                                                                                                       <  1:41192/151‑1 (MQ=255)                                               aTACGCATCTGGATATCGCCACCATCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGa                                                                                                     <  2:44629/151‑1 (MQ=255)                                                 aCGCATCTGGATATCGCCACCATCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGaaa                                                                                                   <  2:185304/151‑1 (MQ=255)                                                  cGCATCTGGATATCGCCACCATCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGaaa                                                                                                   <  1:394615/150‑1 (MQ=255)                                                         ggATATCGCCACCATCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGa                                                                                             <  2:168080/149‑1 (MQ=255)                                                         ggATATCGCCACCATCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGAt                                                                                            <  1:922339/150‑1 (MQ=255)                                                             atCGCCACCATCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTcg                                                                                       >  1:286297/1‑151 (MQ=255)                                                             atCGCCACCATCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTc                                                                                        <  2:479344/150‑1 (MQ=255)                                                                    ccaTCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAg                                                                                 <  2:660618/150‑1 (MQ=255)                                                                      aTCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAg                                                                              <  2:236126/151‑1 (MQ=255)                                                                        cACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGAc                                                                            >  2:579244/1‑151 (MQ=255)                                                                        cACCGGCGGCGTAGCCTATATGAACCACGCGGAAGCGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGGAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGAc                                                                            >  2:627003/1‑151 (MQ=255)                                                                                  gTAGCCTATATGAACCACGAGGAAGTGTTCAGCGAAAATCAGGACATCGTGGCGGAAACGACCGGAATTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCATCGACGAAGCAGAACGTATGCTgg                                                                  >  1:311745/1‑151 (MQ=255)                                                                                    aGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGat                                                                >  1:949566/1‑151 (MQ=255)                                                                                         atatGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATgg                                                            <  2:404205/150‑1 (MQ=255)                                                                                         atatGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATg                                                             <  1:293667/149‑1 (MQ=255)                                                                                          tatGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGc                                                          >  1:211704/1‑151 (MQ=255)                                                                                             gAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCtt                                                        <  2:957683/150‑1 (MQ=255)                                                                                                ccACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCt                                                    <  1:440910/151‑1 (MQ=255)                                                                                                    gcgGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAgg                                                <  2:590247/151‑1 (MQ=255)                                                                                                      ggAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGat                                              <  2:872144/151‑1 (MQ=255)                                                                                                        aaGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGatat                                            >  1:13726/1‑151 (MQ=255)                                                                                                        aaGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGatat                                            >  1:266214/1‑151 (MQ=255)                                                                                                         aGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATc                                           >  2:182264/1‑151 (MQ=255)                                                                                                          gtgtTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCg                                          <  1:689730/151‑1 (MQ=255)                                                                                                            gtTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGa                                         >  1:759428/1‑150 (MQ=255)                                                                                                              tCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAAc                                       >  2:677294/1‑150 (MQ=255)                                                                                                                   gAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTg                                 >  1:788701/1‑151 (MQ=255)                                                                                                                   gAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTg                                 <  2:853163/151‑1 (MQ=255)                                                                                                                   gAAAATCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTg                                 >  2:307833/1‑151 (MQ=255)                                                                                                                      aaTCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTg                              >  2:64778/1‑151 (MQ=255)                                                                                                                       aTCAGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTgg                             <  2:695327/151‑1 (MQ=255)                                                                                                                          aGGACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACAT‑AAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGaa                         <  2:155615/151‑1 (MQ=255)                                                                                                                           ggACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGa                          >  2:811286/1‑150 (MQ=255)                                                                                                                            gACATCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGaaa                        <  2:499206/151‑1 (MQ=255)                                                                                                                               aTCGTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAAcgc                     <  1:353853/151‑1 (MQ=255)                                                                                                                                  gTGGTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCt                  >  2:163519/1‑151 (MQ=255)                                                                                                                                     gTCGCCACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGc               <  1:417215/151‑1 (MQ=255)                                                                                                                                          cACGACCGGACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTaa           >  2:121903/1‑150 (MQ=255)                                                                                                                                                  gACTTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCt  <  2:758406/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GAGCTGGCGATGCAGGTGTCCGATCATGCCCGCGAACTGGCGAAACATACGCATCTGGATATCGCCACCATCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCT  >  NC_000913/2713154‑2713449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑