Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,376,5050AC46.4% 87.2 / 12.7 57T158P (ACC→CCC) ycjRputative TIM alpha/beta barrel enzyme
Reads supporting (aligned to +/- strand):  ref base A (23/7);  new base C (0/26);  total (23/34)
Fisher's exact test for biased strand distribution p-value = 7.85e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATAT  >  NC_000913/1376359‑1376650
                                                                                                                                                       |                                                                                                                                                  
tACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCt                                                                                                                                                >  1:200927/1‑151 (MQ=255)
tACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCt                                                                                                                                                >  1:839677/1‑151 (MQ=255)
    gccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGcc                                                                                                                                            >  2:505368/1‑151 (MQ=255)
       gATGACCTCGCCGCGTAGCCTGGCCGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGAt                                                                                                                                        <  2:257096/151‑1 (MQ=255)
          gACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGc                                                                                                                                      <  1:215872/150‑1 (MQ=255)
            ccTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGccc                                                                                                                                    <  1:126444/150‑1 (MQ=255)
               cgccgcGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCccgcc                                                                                                                                 <  2:843552/150‑1 (MQ=255)
                gccgcGGAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGGCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGt                                                                                                                               >  2:806142/1‑151 (MQ=255)
                     gTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACAt                                                                                                                          >  2:734857/1‑151 (MQ=255)
                       aGCCTGGACGGCGACCGCAAAATGGAGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACAtcg                                                                                                                        >  2:483539/1‑151 (MQ=255)
                            ggACGGCGACCGAAAAATGGTGAGTGATCCCCGGCGCGTACGGGAACAGGTCGCCGCGCGTCCCGGAACGGTGGTGTATCTCGACCCGTTAACCCGCTATCAGGATCATATGATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGaa                                                                                                                   <  2:303964/151‑1 (MQ=255)
                                ggCGACCGCAAAAGGGTGAGTGATCCCCTGCGCGTACGGGAACAGGTCGCCGCGCTTCCCGGAACGGTGGTGTATCCCGACCCGTTAACCCGTTATCAGGATCATATGATCAACCCCCCCGCCGAGGCCCGCCGTTACATCGTCGAAAAc                                                                                                                <  1:825059/150‑1 (MQ=255)
                                ggCGACCGAAAAATGGTGGGTGTTTCCCTGCGCGTACGGGAACAGGCCGCCGCGCGTCCGGGACCGGTGGTGTATCTCGACCCGTAAACCCGCTATCAGGATCATATGATAAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGaaa                                                                                                                  <  2:403773/148‑1 (MQ=255)
                                ggCGACCGAAAAAGGGTGAGTGTTCCCCGGCGCGTAGGGGAACAGGTCGCCGCGCGTCCCGGAACCGTGGTGTATCTCGAACCGTAAACCCGCTATCAGGATAAAATGATCAACCCCCTCGCCGATGCCCGCCGTTACAtc                                                                                                                         <  1:865936/141‑1 (MQ=255)
                                  cGACCGCAAATTGGGGAGTGATTCCCTGGGCGTACTGGAACAGTCCCCCGCGCTTCCCGGACCGGTGGTGTTTCTCAACCCTTAAAACGGTAACCAGAAAAACGTATTCAACCCCCCGCCAGCGGCCCGCCGTTACATCGTCGAAAACGAt                                                                                                             <  2:364338/151‑1 (MQ=255)
                                        aaaaaGGGTGAGTGTTCCCCGGCGCGTACTGAAACAGGTCCCCGCGCGTCCCGGACCCGGGGTGTATCTCGACCCGTTAACCCGCTATCAGGATCATATGATAAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCt                                                                                                           <  2:91525/146‑1 (MQ=255)
                                         aaaaGGGTGAGTGATCCCCTGCGCGTACTGAAACAGGTCCCCGCGCGTCCCGGACCCGTGGTTTTTCTCGACCCTTAAACCCGCTATAAGGATCATATAATCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTaaa                                                                                                       <  2:671975/150‑1 (MQ=255)
                                          aaaTGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAAc                                                                                                      >  2:517376/1‑150 (MQ=255)
                                                         cctgccgcgtcggggAGCAGGCCCCCGCGCGTGCGGGACCGGGGGTTTTTCCCGACCGTTAAACCCGCTATCAGGTTAATATGATCACCCCCCGCCCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCgg                                                                                      <  1:505079/138‑1 (MQ=255)
                                                         ccccGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGACCGGTGGTGTATCTCGAACCGTTAACCCGCTATCAGGATCATATGAACACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACTGATTATCg                                                                                       <  1:56799/150‑1 (MQ=255)
                                                         cccTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCgg                                                                                      >  2:97562/1‑151 (MQ=255)
                                                         cccGGCGCGTCCGGGACCAGGCCGCCGCGCGTCCCGAACCCGTGGTGTATCTCGAACCGTTAACCCGCTATCAGAATCATATGACAAACCCCCTCCCCGATCCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATc                                                                                        <  1:264089/149‑1 (MQ=255)
                                                          ccggggcttccTGAAACAGGTCGCCGCGCGTACCGGAACGGTGGTTTATCCCGACCTTTTAACCCCCTATCAGAATCATATGATCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTAt                                                                                         <  1:651414/137‑1 (MQ=255)
                                                               gcgTACTGGAACGGGTCGCCGCGCGTCCCGGACCGGTGGTTTACCTCGACCCTTAAACCCCTTATCAGGATAATATAATCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGAtt                                                                                 <  2:552526/150‑1 (MQ=255)
                                                                      ggAAGAGGTCGCCGCGCTTACGGGAACGGTGGTTTCTCCCGACCCTTTAACCCGCTACAGGTTTCATAGAACCAACCCCCCCGCGGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATc                                                                           <  2:384168/149‑1 (MQ=255)
                                                                        aacgggccccggcgcgTACGGAACCCGGGGTGTACCCCAACCCTTTAACCCGCTACCGGGATAAAATATCAAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTAt                                                                            <  1:95812/135‑1 (MQ=255)
                                                                        aaCAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACAt                                                                       >  1:814056/1‑151 (MQ=255)
                                                                        aaCAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACAt                                                                       >  2:31816/1‑151 (MQ=255)
                                                                          cAGGTCCCCGCGCGTACCGAAACCGTGGTGTATCCCGAACCTTTAACCCCCTATCAGAAAAATATAATCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATg                                                                      <  1:388784/150‑1 (MQ=255)
                                                                             gTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAat                                                                   >  1:306240/1‑150 (MQ=255)
                                                                               cgccccGCGTCCGGGACCGGTGGTGTATCTCGACCCTTAAACCCTTTAACAGGATAATATGATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAata                                                                  <  1:817894/149‑1 (MQ=255)
                                                                                gccgcGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCg                                                               >  1:27209/1‑151 (MQ=255)
                                                                                 ccggGCGTACCGGACCGGTGTTTTCTCTCGAACCGTAAACCCCCTATCAGGATAATATAACCACCCCCCCCGCGGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAat                                                                   <  2:710043/146‑1 (MQ=255)
                                                                                 ccgcGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAACCCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGaa                                                                    <  2:734460/145‑1 (MQ=255)
                                                                                        tACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaaga                                                           >  1:511406/1‑147 (MQ=255)
                                                                                         aCCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGAt                                                       >  1:699150/1‑150 (MQ=255)
                                                                                            ggAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAAcc                                                   >  2:636278/1‑151 (MQ=255)
                                                                                            gaacccGTGGTGTATCTCGAACCGTTAACCCGCTATCAGAATCATATGATCAACACCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATaa                                                     <  2:914004/145‑1 (MQ=255)
                                                                                                          tctcGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTg                                     >  1:206782/1‑151 (MQ=255)
                                                                                                           cccGAACCGTTAACCCGCTATCAGGATCATATAACCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCt                                      <  2:161333/147‑1 (MQ=255)
                                                                                                              gAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATg                                 >  2:766214/1‑151 (MQ=255)
                                                                                                               aaCCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATg                                 >  1:257365/1‑150 (MQ=255)
                                                                                                                 ccGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGAc                               >  2:307026/1‑150 (MQ=255)
                                                                                                                          ccGCTATCAGAATCATATGATCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACcgcg                        <  2:856351/149‑1 (MQ=255)
                                                                                                                               tataAGGATAATATAATAAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCt                    <  2:435823/149‑1 (MQ=255)
                                                                                                                                   cAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCg               >  2:844402/1‑151 (MQ=255)
                                                                                                                                    aGGATATTATTACCAACCCCCCCGCCGGTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTc                <  1:324904/149‑1 (MQ=255)
                                                                                                                                    aGGATAATTTGATAAACACCCTCGCCGATCGCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCt                 <  2:39107/148‑1 (MQ=255)
                                                                                                                                      gattCATATAACCAACCCCCCCGCCGATCCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCt                 <  1:2873/144‑1 (MQ=255)
                                                                                                                                           atatGACCAACCCCCCCGCCGTTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAtg         <  1:266650/150‑1 (MQ=255)
                                                                                                                                            tatGACCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTc            <  2:840907/146‑1 (MQ=255)
                                                                                                                                             atGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGc      >  2:264497/1‑151 (MQ=255)
                                                                                                                                             atGACCAACCCCCACGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAt          <  1:223326/147‑1 (MQ=255)
                                                                                                                                              tGATCACCCCCCGCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGc      <  2:906182/150‑1 (MQ=255)
                                                                                                                                               tgacaaaCCCCCGCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAt          <  2:908442/141‑1 (MQ=255)
                                                                                                                                                aTCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCat    >  2:556456/1‑150 (MQ=255)
                                                                                                                                                 tCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCatat  >  2:139692/1‑151 (MQ=255)
                                                                                                                                                       |                                                                                                                                                  
TACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATAT  >  NC_000913/1376359‑1376650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: