Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,376,505 | 0 | A | C | 46.4% | 87.2 / 12.7 | 57 | T158P (ACC→CCC) | ycjR | putative TIM alpha/beta barrel enzyme |
Reads supporting (aligned to +/- strand): ref base A (23/7); new base C (0/26); total (23/34) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.85e-10 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGC‑T‑ATCAGG‑ATCATATGATC‑AACACCCTCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATAT > NC_000913/1376359‑1376650 | tACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGC‑T‑ATCAGG‑ATCATATGATC‑AACACCCt > 1:200927/1‑151 (MQ=255) tACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGC‑T‑ATCAGG‑ATCATATGATC‑AACACCCt > 1:839677/1‑151 (MQ=255) gccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGC‑T‑ATCAGG‑ATCATATGATC‑AACACCCTCGcc > 2:505368/1‑151 (MQ=255) gATGACCTCGCCGCGTAGCCTGGCCGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGC‑T‑ATCAGG‑ATCATATGATC‑AACACCCTCGCC‑GAt < 2:257096/151‑1 (MQ=255) gACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGC‑T‑ATCAGG‑ATCATATGATC‑AACACCCTCGCC‑GATGc < 1:215872/150‑1 (MQ=255) ccTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGC‑T‑ATCAGG‑ATCATATGATC‑AACACCCTCGCC‑GATGccc < 1:126444/150‑1 (MQ=255) cgccgcGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGC‑T‑ATCAGG‑ATCATATGATC‑AACACCCTCGCC‑GATGCccgcc < 2:843552/150‑1 (MQ=255) gccgcGGAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGGCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGC‑T‑ATCAGG‑ATCATATGATC‑AACACCCTCGCC‑GATGCCCGCCGt > 2:806142/1‑151 (MQ=255) gTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGC‑T‑ATCAGG‑ATCATATGATC‑AACACCCTCGCC‑GATGCCCGCCGTTACAt > 2:734857/1‑151 (MQ=255) aGCCTGGACGGCGACCGCAAAATGGAGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGC‑T‑ATCAGG‑ATCATATGATC‑AACACCCTCGCC‑GATGCCCGCCGTTACAtcg > 2:483539/1‑151 (MQ=255) ggACGGCGACCGAAAAATGGTGAGTGATCCCCGGCGCGTACGGGAACAGGTCGCCGCGCGTCCCGGAACGGTGGTGTATCTCGACCCGTTAAC‑CCGC‑T‑ATCAGG‑ATCATATGATC‑AACCCCCTCGCC‑GATGCCCGCCGTTACATCGTCGaa < 2:303964/151‑1 (MQ=255) ggCGACCGCAAAAGGGTGAGTGATCCCCTGCGCGTACGGGAACAGGTCGCCGCGCTTCCCGGAACGGTGGTGTATCCCGACCCGTTAAC‑CCGT‑T‑ATCAGG‑ATCATATGATC‑AACCCCCCCGCC‑GAGGCCCGCCGTTACATCGTCGAAAAc < 1:825059/150‑1 (MQ=255) ggCGACCGAAAAATGGTGGGTGTTTCCCTGCGCGTACGGGAACAGGCCGCCGCGCGTCCGGGACCGGTGGTGTATCTCGACCCGTAAAC‑CCGC‑T‑ATCAGG‑ATCATATGATA‑AACCCCCTCGCC‑GATGCCCGCCGTTACATCGTCGaaa < 2:403773/148‑1 (MQ=255) ggCGACCGAAAAAGGGTGAGTGTTCCCCGGCGCGTAGGGGAACAGGTCGCCGCGCGTCCCGGAACCGTGGTGTATCTCGAACCGTAAAC‑CCGC‑T‑ATCAGG‑ATAAAATGATC‑AACCCCCTCGCC‑GATGCCCGCCGTTACAtc < 1:865936/141‑1 (MQ=255) cGACCGCAAATTGGGGAGTGATTCCCTGGGCGTACTGGAACAGTCCCCCGCGCTTCCCGGACCGGTGGTGTTTCTC‑AACCCTTAAA‑ACGG‑TAACCAGA‑AAAACGTATTC‑AAC‑CCCCCGCCAGCGGCCCGCCGTTACATCGTCGAAAACGAt < 2:364338/151‑1 (MQ=255) aaaaaGGGTGAGTGTTCCCCGGCGCGTACTGAAACAGGTCCCCGCGCGTCCCGGACCCGGGGTGTATCTCGACCCGTTAAC‑CCGC‑T‑ATCAGG‑ATCATATGATA‑AACCCCCCCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCt < 2:91525/146‑1 (MQ=255) aaaaGGGTGAGTGATCCCCTGCGCGTACTGAAACAGGTCCCCGCGCGTCCCGGACCCGTGGTTTTTCTCGACCCTTAAAC‑CCGC‑T‑ATAAGG‑ATCATATAATC‑AACCCCCCCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTaaa < 2:671975/150‑1 (MQ=255) aaaTGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGC‑T‑ATCAGG‑ATCATATGATC‑AACACCCTCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAAc > 2:517376/1‑150 (MQ=255) cctgccgcgtcggggAGCAGGCCCCCGCGCGTGCGGGACCGGGGGTTTTTCCCG‑ACCGTTAAACCCGC‑T‑ATCAGG‑TTAATATGATC‑ACCCCCCGCCCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCgg < 1:505079/138‑1 (MQ=255) ccccGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGACCGGTGGTGTATCTCGAACCGTTAAC‑CCGC‑T‑ATCAGG‑ATCATATGAAC‑ACCCCCCCCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACTGATTATCg < 1:56799/150‑1 (MQ=255) cccTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGC‑T‑ATCAGG‑ATCATATGATC‑AACACCCTCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCgg > 2:97562/1‑151 (MQ=255) cccGGCGCGTCCGGGACCAGGCCGCCGCGCGTCCCGAACCCGTGGTGTATCTCGAACCGTTAAC‑CCGC‑T‑ATCAGA‑ATCATATGACA‑AACCCCCTCCCC‑GATCCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATc < 1:264089/149‑1 (MQ=255) ccggggcttccTGAAACAGGTCGCCGCGCGTACCGGAACGGTGGTTTATCCCGACCTTTTAAC‑CCCC‑T‑ATCAGA‑ATCATATGATC‑AACCCCCCCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTAt < 1:651414/137‑1 (MQ=255) gcgTACTGGAACGGGTCGCCGCGCGTCCCGGACCGGTGGTTTACCTCG‑ACCCTTAAA‑CCCCTT‑ATCAGG‑ATAATATAATC‑AACCCCCCCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGAtt < 2:552526/150‑1 (MQ=255) ggAAGAGGTCGCCGCGCTTACGGGAACGGTGGTTTCTCCCGACCCTTTAAC‑CCGC‑T‑A‑CAGGTTTCATAGAACC‑AACCCCCCCGCG‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATc < 2:384168/149‑1 (MQ=255) aacgggccccggcgcgTACGGAACCCGGGGTGTACCCCAACCCTTTAAC‑CCGC‑T‑ACCGGG‑ATAAAAT‑ATCAAACCCCCCCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTAt < 1:95812/135‑1 (MQ=255) aaCAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGC‑T‑ATCAGG‑ATCATATGATC‑AACACCCTCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACAt > 1:814056/1‑151 (MQ=255) aaCAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGC‑T‑ATCAGG‑ATCATATGATC‑AACACCCTCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACAt > 2:31816/1‑151 (MQ=255) cAGGTCCCCGCGCGTACCGAAACCGTGGTGTATCCCGAACCTTTAAC‑CCCC‑T‑ATCAGA‑AAAATATAATC‑ACCCCCCCCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATg < 1:388784/150‑1 (MQ=255) gTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGC‑T‑ATCAGG‑ATCATATGATC‑AACACCCTCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAat > 1:306240/1‑150 (MQ=255) cgccccGCGTCCGGGACCGGTGGTGTATCTCGACCCTTAAAC‑CCTT‑T‑AACAGG‑ATAATATGATC‑AACCCCCTCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAata < 1:817894/149‑1 (MQ=255) gccgcGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGC‑T‑ATCAGG‑ATCATATGATC‑AACACCCTCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCg > 1:27209/1‑151 (MQ=255) ccggGCGTACCGGACCGGTGTTTTCTCTCGAACCGTAAAC‑CCCC‑T‑ATCAGG‑ATAATATAACC‑ACCCCCCCCGCG‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAat < 2:710043/146‑1 (MQ=255) ccgcGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAC‑CCGC‑T‑ATCAGG‑ATCATATGATC‑AACACCCTCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGaa < 2:734460/145‑1 (MQ=255) tACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGC‑T‑ATCAGG‑ATCATATGATC‑AACACCCTCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaaga > 1:511406/1‑147 (MQ=255) aCCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGC‑T‑ATCAGG‑ATCATATGATC‑AACACCCTCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGAt > 1:699150/1‑150 (MQ=255) ggAACCGTGGTGTATCTCGAACCGTTAAA‑CCGC‑T‑ATCAGG‑ATCATATGATC‑AACACCCTCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAAcc > 2:636278/1‑151 (MQ=255) gaacccGTGGTGTATCTCGAACCGTTAAC‑CCGC‑T‑ATCAGA‑ATCATATGATC‑AACACCCCCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATaa < 2:914004/145‑1 (MQ=255) tctcGAACCGTTAAA‑CCGC‑T‑ATCAGG‑ATCATATGATC‑AACACCCTCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTg > 1:206782/1‑151 (MQ=255) cccGAACCGTTAAC‑CCGC‑T‑ATCAGG‑ATCATATAACC‑AACCCCCCCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCt < 2:161333/147‑1 (MQ=255) gAACCGTTAAA‑CCGC‑T‑ATCAGG‑ATCATATGATC‑AACACCCTCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATg > 2:766214/1‑151 (MQ=255) aaCCGTTAAA‑CCGC‑T‑ATCAGG‑ATCATATGATC‑AACACCCTCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATg > 1:257365/1‑150 (MQ=255) ccGTTAAA‑CCGC‑T‑ATCAGG‑ATCATATGATC‑AACACCCTCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGAc > 2:307026/1‑150 (MQ=255) ccGC‑T‑ATCAGA‑ATCATATGATC‑ACCCCCCCCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACcgcg < 2:856351/149‑1 (MQ=255) t‑ataAGG‑ATAATATAATA‑AACCCCCTCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCt < 2:435823/149‑1 (MQ=255) cAGG‑ATCATATGATC‑AACACCCTCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCg > 2:844402/1‑151 (MQ=255) aGG‑ATATTATTACC‑AACCCCCCCGCC‑GGTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTc < 1:324904/149‑1 (MQ=255) aGG‑ATAATTTGATA‑AACACCCTCGCC‑GATCGCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCt < 2:39107/148‑1 (MQ=255) gattCATATAACC‑AACCCCCCCGCC‑GATCCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCt < 1:2873/144‑1 (MQ=255) atatGACC‑AACCCCCCCGCC‑GTTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAtg < 1:266650/150‑1 (MQ=255) tatGACC‑AACCCCCTCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTc < 2:840907/146‑1 (MQ=255) atGATC‑AACACCCTCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGc > 2:264497/1‑151 (MQ=255) atGACC‑AACCCCCACGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAt < 1:223326/147‑1 (MQ=255) tGATC‑ACCCCCCGCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGc < 2:906182/150‑1 (MQ=255) tgacaaaCCCCCGCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAt < 2:908442/141‑1 (MQ=255) aTC‑AACACCCTCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCat > 2:556456/1‑150 (MQ=255) tC‑AACACCCTCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCatat > 2:139692/1‑151 (MQ=255) | TACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGC‑T‑ATCAGG‑ATCATATGATC‑AACACCCTCGCC‑GATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATAT > NC_000913/1376359‑1376650 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |