Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,898,100	0	T	G	63.0%	30.8 / 57.8	46	V390G (GTG→GGG)	sdaA	L‑serine dehydratase 1
Reads supporting (aligned to +/- strand):  ref base T (8/9);  new base G (11/18);  total (19/27)
Fisher's exact test for biased strand distribution p-value = 7.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

AAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGTGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAA  >  NC_000913/1897956‑1898244                                                                                                                                                 |                                                                                                                                                 aaGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGTGCCGTg                                                                                                                                            >  2:264465/1‑151 (MQ=255) aaGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGGGCCGTg                                                                                                                                            <  2:652651/151‑1 (MQ=255)       gtgtTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGGGCCGTGCATTGa                                                                                                                                      <  1:529766/151‑1 (MQ=255)           tGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGGGCCGTGCATTGAGCGt                                                                                                                                  >  2:179296/1‑151 (MQ=255)                gTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGGGCCGTGCATTGAGCGTAAt                                                                                                                               <  1:245053/149‑1 (MQ=255)                gTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGGGCCGTGCATTGAGCGTAATg                                                                                                                              <  1:766580/150‑1 (MQ=255)                gTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGGGCCGTGCATTGAGCGTAATGc                                                                                                                             <  1:202928/151‑1 (MQ=255)                   cAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGGGCCGTGCATTGAGCGTAATGCCAt                                                                                                                          <  2:168692/151‑1 (MQ=255)                    aaTGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGTGCCGTGCATTGAGCGTAATGCCAtt                                                                                                                         <  2:287748/151‑1 (MQ=255)                             tgcGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGGGCCGTGCATTGAGCGTAATGCCATTGCCTCtgtg                                                                                                                >  1:902066/1‑151 (MQ=255)                               cGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGGGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGaa                                                                                                              <  2:259588/151‑1 (MQ=255)                                       gCAGAACTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGTGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGAtt                                                                                                       >  2:593857/1‑150 (MQ=255)                                       gCAGAACTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGGGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGAtt                                                                                                       >  2:777874/1‑150 (MQ=255)                                        cAGAACTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGTGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTaa                                                                                                     <  2:877015/151‑1 (MQ=255)                                        cAGAACTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGGGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTa                                                                                                      >  1:142757/1‑150 (MQ=255)                                           aaCTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGTGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAAcgc                                                                                                  <  1:30422/151‑1 (MQ=255)                                           aaCTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGGGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAAcgc                                                                                                  <  1:278028/151‑1 (MQ=255)                                                    gCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGGGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGAt                                                                                         <  1:780337/151‑1 (MQ=255)                                                     cGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGTGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATg                                                                                        <  1:897375/151‑1 (MQ=255)                                                      ggTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGTGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATgg                                                                                       <  2:667772/151‑1 (MQ=255)                                                            ccGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGTGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTgc                                                                                 >  1:494832/1‑151 (MQ=255)                                                             cGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGTGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTgcg                                                                                <  2:189432/151‑1 (MQ=255)                                                               gAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGCTTAACCTGCGACCCGGCTGCAGGGCAGGTTCAGGTGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATCAACGCAGCGCGGATGGCTCTGcgcc                                                                              >  1:105246/1‑151 (MQ=255)                                                                     gTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGGGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCAcc                                                                         >  1:9238/1‑150 (MQ=255)                                                                       ttGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGGGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGt                                                                      >  1:47240/1‑151 (MQ=255)                                                                          cGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGGGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGc                                                                    >  1:536516/1‑150 (MQ=255)                                                                             ggcggcGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGGGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCAcc                                                                 <  1:21243/150‑1 (MQ=255)                                                                               cggcggAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGGGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACcgcg                                                              <  2:435553/151‑1 (MQ=255)                                                                                  cggAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGGGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGt                                                           >  1:744293/1‑151 (MQ=255)                                                                                     aaaTTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGGGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGtctc                                                        <  1:243752/151‑1 (MQ=255)                                                                                     aaaTTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGGGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGtctc                                                        <  2:333094/151‑1 (MQ=255)                                                                                      aaTTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGTGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCg                                                       <  1:779334/151‑1 (MQ=255)                                                                                          ggCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGGGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTgg                                                   >  2:462120/1‑151 (MQ=255)                                                                                           gCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGTGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTgg                                                   >  1:76537/1‑150 (MQ=255)                                                                                             aTGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGGGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATa                                                <  2:434098/151‑1 (MQ=255)                                                                                              tGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGTGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATa                                                >  2:4903/1‑150 (MQ=255)                                                                                               ggAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGGGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATaa                                               <  2:515522/150‑1 (MQ=255)                                                                                                    acaACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGGGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCAt                                         <  1:274368/151‑1 (MQ=255)                                                                                                     caACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGGGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATc                                        <  2:221117/151‑1 (MQ=255)                                                                                                        ccTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGTGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGaa                                     <  2:832387/151‑1 (MQ=255)                                                                                                                   ccTGCGACCCGGTTGCAGGGCAGGTTCAGGTGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACg                           >  2:345756/1‑150 (MQ=255)                                                                                                                         aCCCGGTTGCAGGGCAGGTTCAGGGGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCgg                    >  2:744983/1‑151 (MQ=255)                                                                                                                           ccGGTTGCAGGGCAGGTTCAGGGGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGt                   >  1:600803/1‑150 (MQ=255)                                                                                                                              gTTGCAGGGCAGGTTCAGGTGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAgg               >  2:78809/1‑151 (MQ=255)                                                                                                                                      gcaggTTCAGGGGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAAc       <  2:120967/151‑1 (MQ=255)                                                                                                                                            tCAGGTGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCaa  <  1:363185/150‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                 AAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGTGCCGTGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAA  >  NC_000913/1897956‑1898244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑