Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,042,6040AC45.9% 104.8 / 15.8 61V182G (GTG→GGG) yqfAhemolysin III family HylIII inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (26/7);  new base C (0/28);  total (26/35)
Fisher's exact test for biased strand distribution p-value = 3.60e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGT  >  NC_000913/3042455‑3042745
                                                                                                                                                      |                                                                                                                                              
ggCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCa                                                                                                                                                <  1:61378/150‑1 (MQ=255)
ggCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCAc                                                                                                                                               <  1:569210/151‑1 (MQ=255)
   gcgTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCAcccc                                                                                                                                            >  2:18716/1‑151 (MQ=255)
   gcgTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCAcccc                                                                                                                                            <  2:683153/151‑1 (MQ=255)
         tATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGc                                                                                                                                       <  2:373670/149‑1 (MQ=255)
            cAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATa                                                                                                                                  <  1:837280/151‑1 (MQ=255)
             aGGCCTACAAAGGCATCCCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATa                                                                                                                                  <  1:511899/150‑1 (MQ=255)
             aGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATa                                                                                                                                  >  2:15897/1‑150 (MQ=255)
               gCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAAc                                                                                                                               >  2:271034/1‑151 (MQ=255)
                   aCAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCAc                                                                                                                            <  2:253701/150‑1 (MQ=255)
                     aaaGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCAcgcc                                                                                                                         >  1:500807/1‑151 (MQ=255)
                      aaGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCAcgccg                                                                                                                        >  2:813474/1‑151 (MQ=255)
                          cATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACgccgcc                                                                                                                      >  1:235322/1‑149 (MQ=255)
                               ccATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGcc                                                                                                               >  2:69802/1‑151 (MQ=255)
                                     cGCCAGCCTAATATCAAAATAGATCACCAGAAGTTGGCACCCACACCCGCCGATCCCGAGCCCGTGCGATATGGCCTGATTGTATGGAATGCGTTTGCAGACGTAAAAAATCCCCCCGCGCGAATAACCCACGCCGCCTACCGCCAGt                                                                                                            <  1:578768/148‑1 (MQ=255)
                                          gCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAAc                                                                                                    >  1:605663/1‑151 (MQ=255)
                                           cccAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAAc                                                                                                    >  2:474891/1‑150 (MQ=255)
                                             cAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCt                                                                                                 >  2:94372/1‑151 (MQ=255)
                                               atatACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGc                                                                                               >  1:786000/1‑151 (MQ=255)
                                                tataCAAATAGCCCGCAAGAAAGTGGCACACACTACCGCCGAGCTCGAATCCGTGCCAGATGGCATGGTTGTATGGAATGGGTTTGCAGACGTAAAAAATCACCCCGAGCGAATAACCCGCGCCGCCTACCGCCAGTAAGGTAAc                                                                                                    <  1:418532/145‑1 (MQ=255)
                                                    cAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCCCCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCccgc                                                                                           <  2:234201/150‑1 (MQ=255)
                                                      aaTAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCcgccgc                                                                                        >  1:731871/1‑151 (MQ=255)
                                                       aTAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCcgcg                                                                                       >  2:563126/1‑151 (MQ=255)
                                                             cGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCtt                                                                                  >  1:389223/1‑150 (MQ=255)
                                                             cGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCtt                                                                                  >  2:534283/1‑150 (MQ=255)
                                                               ccAGAAGGGGCCACCCACTCCCGCCGAGCACGAAGCCGGCCCAGATGGCTGGGTTGTAGGGAAGGCGTTGGCAGACGTAAAAATTCCCCCCGGGCGAAAAAACCCCCCCCCTTCCCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTa                                                                                 <  1:168914/149‑1 (MQ=255)
                                                                 aGAAAGGGCCACACTCTACCGCCAGGCACGAAGCCGTGCCAGAGGGCATGTTTGTATGGAATGCGTTTGCAGACGAAAAAAACCCCCCCGAGCAAAAAACCCCCCCCGCCTCCCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACt                                                                              <  2:401956/150‑1 (MQ=255)
                                                                   aaggggccacaccCTCCCCCGGACCACGAGCCGGTGCAAGAGGCCTGGGTTTTATGGAAGGCGTTTGAAGCCGGAAAAAACCCCCCCGAGCAAAAAACCCCCCCCCCTTCCCGCCAGTAAGGTAACGCTGCCCGCCGCGAg                                                                                     <  1:358223/134‑1 (MQ=255)
                                                                    aagggccacacccccccgccgAGCACGAAGCCGTGCAAGATGGCATGGTTGTATGGAATGCTTTTGCAGACGTAAAAAATCCCCCCGAGCAAATAACCCCCGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCa                                                                          <  1:177155/136‑1 (MQ=255)
                                                                     gggggCCCACCCTCCCGCCGAGCACAAACCCGTGCAAGAGGGCATGGTTGTAGGGAATGCGTTTGAAGACGTAAAAAACCCCCCCGAGCAAAAAACCCCCCCCGCTTCCCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCAt                                                                         <  1:354111/148‑1 (MQ=255)
                                                                            cacacTCCCGCCGAGCACGAAGCCGTGCCAGATGGCATGTTTGTATGGAATGCGTTTGCAGACGTAGAAAATCCCCCCGAGCGAATAAACCCCGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATaa                                                                  <  2:677961/151‑1 (MQ=255)
                                                                               acTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAAtt                                                               >  2:916835/1‑151 (MQ=255)
                                                                                   ccgccgAGCCCGAAGCCGTGCCAGAGGGCATGTTTGTAGGGAATGCGTTTGCAGACGTAGAAAACCCCCCCGAGCAAAAAACCCACGCCGCCTCCCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAAt                                                                <  2:700139/146‑1 (MQ=255)
                                                                                   ccgccgAGCACGAAGCCGTGCCAGATGGCATGTTTTTATGAAATGCTTTTGCAGACGTAGAAAATCCCCCCGAGCAAATAACCCCCGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAAtt                                                               <  2:808127/147‑1 (MQ=255)
                                                                                      ccgAGCCCGAAGCCGTGCCAGATGGCTTGGTTTTATGGAATGCTTTTGCAGACGTAAAAAACCCCCCCGAGCAAATAACCCCCGCCGCCTCCCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTaccacc                                                         <  2:445824/150‑1 (MQ=255)
                                                                                            acaacgCCGAGCCAGATGGCAGGTTTATATGAAATGCTTTGGCAGACGAAAAAAACCCCCCCGGGGAAAAAACCCCCCCCCCCTCCCCCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCg                                                     <  1:383153/143‑1 (MQ=255)
                                                                                             cGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGAc                                                   >  2:231125/1‑149 (MQ=255)
                                                                                               aggcggTGCCAGAGGGCTTGGTTTTAGGAAATGCTTTTGCAGACGTAAAAAACCCCCCCGAGCAAAAAACCCCCCCCGCCTCCCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGc                                                      <  1:758369/139‑1 (MQ=255)
                                                                                               aggCCGGGCCAGATGGCTGGTTTGTAGGAATGGCGTTTGCAGGTGTAGAAAATACCCCCGGGCGAATAACCCCCGCCCCCTCCCGCCAGTAAGGAAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGAc                                                   <  2:777865/145‑1 (MQ=255)
                                                                                                 gCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGAcagcc                                               >  1:613952/1‑149 (MQ=255)
                                                                                                  cggtcccAGATGCCATGGTTGTATGAAATGCGTTGGAAGCCGTAGAAAACCCCCCCGACCGAAAAAACCACGCCCCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGAcagcca                                              <  2:203905/144‑1 (MQ=255)
                                                                                                  ccGTGCAAGATGGCATGGTTGTATGGAATGCGTTTGCAGCCGAAAAAAACCCCCCCGAGCAAAAAACCCCCCCCCCCTCCCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAg                                                       <  1:456861/140‑1 (MQ=255)
                                                                                                     tGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGccc                                          >  2:778509/1‑150 (MQ=255)
                                                                                                            tggctgggTTTTATGGAATGCTTTTGCGGCCTTAGAAAATCCCCCCGGGCAAATAACCCCCCCCGCCTCCCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCa                                   <  1:906783/144‑1 (MQ=255)
                                                                                                            tGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGcc                                    >  1:400784/1‑149 (MQ=255)
                                                                                                             ggCATGGTTGTATGAAATGCTTTTGAAAACTAAAAAAACCCCCCCGAGCAAAAAACCCCCCCCCCCTCCCGCCAGAAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCa                                         <  1:793845/143‑1 (MQ=255)
                                                                                                             ggCATGGTTGTAGGGAATGCGTTTGCAGACGTAAAAAACCCCCCCGAGCGAATAACCCACGCCGCCTCCCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGc                                     <  2:503239/147‑1 (MQ=255)
                                                                                                               cATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGAt                                >  2:221572/1‑150 (MQ=255)
                                                                                                                 tGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAgg                             >  2:401758/1‑151 (MQ=255)
                                                                                                                  gtttttacgaaaTGTGTTTGCAGATGTAGAAAATCCCCCCGCGCGAATAACCCCCCCCGCTTCCCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGAt                                <  2:31484/137‑1 (MQ=255)
                                                                                                                  ggTTGTATGAAATGGTTTTGCAGACGTAAAATTCCCCCCCGGGCAAATAACCCCCGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATa                               <  2:547443/148‑1 (MQ=255)
                                                                                                                  ggTTGTATGAAATGCGTTTGCAGACGTAAAAAACCCCCCCGAGCGAATAACCCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAg                              <  2:882819/149‑1 (MQ=255)
                                                                                                                  ggTTGTAGGAAATGCGTTTGAAGACGTAAAAAACCCCCCCGGGCAAAAAACCCCCGCCCCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAg                              <  1:467361/149‑1 (MQ=255)
                                                                                                                      gTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCAcc                        >  1:568657/1‑151 (MQ=255)
                                                                                                                       tatgataTGCGTTTCCAGCGGTAAAAAACCCCCCCGAGCGAAAAACCCCCCCCCCCACCCGCCAGTAAGGTAACGCCGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGa                                 <  1:512897/135‑1 (MQ=255)
                                                                                                                       tATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCAcc                        >  2:96679/1‑150 (MQ=255)
                                                                                                                          ggAATGCGTTTGCAGACGTAAAAAACCCCCCCGAGCGAAAAACCCCCGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCagag                    <  2:219817/151‑1 (MQ=255)
                                                                                                                          ggAATGCGTTTGAAGCCGTAAAAAACCCCCCCGAGCAAATAACCCCCCCCCCTTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCAcc                        <  2:634478/147‑1 (MQ=255)
                                                                                                                                gCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATaa                >  2:845726/1‑150 (MQ=255)
                                                                                                                                 cGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATaaa               >  2:911168/1‑150 (MQ=255)
                                                                                                                                              aaaaaaCCCCCCCGGGCGAATAACCCCCGCCCCCTCCCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGt  <  2:67688/148‑1 (MQ=255)
                                                                                                                                                 aaaTCCCCCCGGAGCAAAAAACCCCCGCCGCTTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGa        <  1:49784/142‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                              
GGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGT  >  NC_000913/3042455‑3042745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: