Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,984,547 A→C S175A (TCT→GCT)  araG ← L‑arabinose ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,984,5470AC100.0% 209.0 / NA 57S175A (TCT→GCT) araGL‑arabinose ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (33/24);  total (33/24)

TTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCGGGTCAATATCCATACCAAGATGTTTAAGTTGTAA  >  NC_000913/1984401‑1984694
                                                                                                                                                  |                                                                                                                                                   
tttGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAgg                                                                                                                                                 >  1:241979/1‑151 (MQ=255)
      aTACCGCCCACCTTTAAAGACAGTAATGGCATCGCGGAGGGCAAATTTTTCTTCCATCCGGTGAGAAACGTATAAGATTCCCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTg                                                                                                                                           <  2:115881/151‑1 (MQ=255)
      aTAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTg                                                                                                                                           >  2:858806/1‑151 (MQ=255)
      aTAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTg                                                                                                                                           >  1:437650/1‑151 (MQ=255)
       tAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTgg                                                                                                                                          >  1:490638/1‑151 (MQ=255)
               aTCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCtc                                                                                                                                   <  2:168047/150‑1 (MQ=255)
                   ttAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCtcatca                                                                                                                               >  1:557344/1‑150 (MQ=255)
                   ttAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCtcatca                                                                                                                               >  1:325956/1‑150 (MQ=255)
                    tAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCaa                                                                                                                              >  2:691506/1‑150 (MQ=255)
                     aaaGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCaa                                                                                                                              >  1:435061/1‑149 (MQ=255)
                      aaGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAgg                                                                                                                           >  2:495978/1‑151 (MQ=255)
                          cAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGAt                                                                                                                       >  2:510620/1‑151 (MQ=255)
                           aGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGAt                                                                                                                       <  2:873980/150‑1 (MQ=255)
                                tGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAAtttt                                                                                                                 >  2:795742/1‑151 (MQ=255)
                                  gCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGc                                                                                                              >  2:795377/1‑151 (MQ=255)
                                         tGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTAcg                                                                                                        >  1:886940/1‑151 (MQ=255)
                                            gggCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACgcgc                                                                                                     >  1:631409/1‑151 (MQ=255)
                                               cAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGcc                                                                                                    >  1:292129/1‑149 (MQ=255)
                                                aaaTATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAgc                                                                                                 <  2:460378/151‑1 (MQ=255)
                                                    atTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCtt                                                                                             >  1:780457/1‑151 (MQ=255)
                                                         ttcCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCg                                                                                        >  1:175002/1‑151 (MQ=255)
                                                         ttcCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCg                                                                                        >  2:203626/1‑151 (MQ=255)
                                                         ttcCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCg                                                                                        >  1:858212/1‑151 (MQ=255)
                                                         tcccATCCGGTGAAAACTGTAAAATATCTCCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTg                                                                                           <  1:366274/146‑1 (MQ=255)
                                                           ccATACGGTGAGAAACGTAAAAGATTCCCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCg                                                                                        <  2:46489/149‑1 (MQ=255)
                                                             aTACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGAttt                                                                                    >  1:871946/1‑151 (MQ=255)
                                                                   tGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAAcc                                                                               >  2:208312/1‑150 (MQ=255)
                                                                            tataAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACt                                                                     >  1:505517/1‑151 (MQ=255)
                                                                             ataAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACt                                                                     >  1:902835/1‑150 (MQ=255)
                                                                               aaGATTCCCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGAc                                                                  <  1:858959/151‑1 (MQ=255)
                                                                                   ttACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAAt                                                              >  2:118117/1‑151 (MQ=255)
                                                                                    tACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCa                                                                >  2:341453/1‑148 (MQ=255)
                                                                                     aCCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGAcc                                                                 <  1:519363/146‑1 (MQ=255)
                                                                                         gCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGgag                                                          >  2:16128/1‑149 (MQ=255)
                                                                                                    cGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAgcg                                             <  1:865099/151‑1 (MQ=255)
                                                                                                      cAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAgcggc                                           <  2:146568/151‑1 (MQ=255)
                                                                                                       aGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAgcgg                                            >  2:644801/1‑149 (MQ=255)
                                                                                                            aCGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTcc                                     <  1:372844/151‑1 (MQ=255)
                                                                                                            aCGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTcc                                     <  2:336803/151‑1 (MQ=255)
                                                                                                              gAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCgg                                   >  2:53119/1‑151 (MQ=255)
                                                                                                                aTAACGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCGGGt                                 <  1:428838/151‑1 (MQ=255)
                                                                                                                  aaCGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCGGGTc                                <  2:203083/150‑1 (MQ=255)
                                                                                                                  aaCGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCGGGTCa                               <  2:437665/151‑1 (MQ=255)
                                                                                                                   aCGCGGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCGGGTCaa                              <  1:929466/151‑1 (MQ=255)
                                                                                                                     gcgGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCGGGTc                                >  1:761806/1‑147 (MQ=255)
                                                                                                                     gcgGAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCGGGTCAata                            <  1:124060/151‑1 (MQ=255)
                                                                                                                       ggAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGGCCGGGGCAATATc                          >  2:77137/1‑151 (MQ=255)
                                                                                                                        gAAAAGATTGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCGGGTCAATATcc                         <  2:347014/151‑1 (MQ=255)
                                                                                                                                tGTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCGGGTCAATATCCATACCa                   >  1:699744/1‑149 (MQ=255)
                                                                                                                                 gTCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCGGGTCAATATCCATACCAAGa                <  2:490653/151‑1 (MQ=255)
                                                                                                                                  tCGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCGGGTCAATATCCATACCa                   >  1:667116/1‑147 (MQ=255)
                                                                                                                                   cGATTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCGGGTCAATATCCATACCAAGAt               <  1:374383/150‑1 (MQ=255)
                                                                                                                                     aTTTCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCGGGTCAATATCCATACCAAGATGtt            >  1:297407/1‑151 (MQ=255)
                                                                                                                                       ttCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCGGGTCAATATCCATACCAAGATGttt           <  2:472874/150‑1 (MQ=255)
                                                                                                                                       ttCACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCGGGTCAATATCCATACCAAGATGttt           <  1:781021/150‑1 (MQ=255)
                                                                                                                                         cACGGGCAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCGGGTCAATATCCATACCAAGATGTTTAAg        <  2:99991/151‑1 (MQ=255)
                                                                                                                                               cAGCGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCGGGTCAATATCCATACCAAGATGTTTAAGTTGTaa  <  1:872703/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACTGACCAATGGAGAGATATTTCAGCGGCGTGTCCGGGTCAATATCCATACCAAGATGTTTAAGTTGTAA  >  NC_000913/1984401‑1984694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: