Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,004,251 A→C K127T (AAA→ACA)  fliD → flagellar filament capping protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,004,2510AC97.8% 157.8 / NA 45K127T (AAA→ACA) fliDflagellar filament capping protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (20/24);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CCACCGCGTTCAGTGCCACCACTGCGGGTAACGCCATCGCCGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTG  >  NC_000913/2004107‑2004394
                                                                                                                                                |                                                                                                                                               
ccaccGCGTTCAGTGCCACCACTGCGGGTAACGCCATCGCCGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCAc                                                                                                                                           >  1:835043/1‑151 (MQ=255)
   ccGCGTTCAGTGCCACCACTGCGGGTAACGCCATCGCCGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCAt                                                                                                                                        >  2:270895/1‑151 (MQ=255)
            gTGCCACCACTGCGGGTAACGCCATCGCCGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAgg                                                                                                                               >  1:780896/1‑151 (MQ=255)
              gCCACCCCTGCGGGTAACGCCATCGCCGGCAAATACACCATCAGCGTCACCCATCCGGCGCAGGCGCAACCCCTCACCACGCGCACCGCCAGCGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACTCTCACCATTCAACAAggcg                                                                                                                             <  1:703579/151‑1 (MQ=255)
              gCCACCACTGCGGGTAACGCCATCGCCGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAggcg                                                                                                                             >  2:238478/1‑151 (MQ=255)
                caccacTGCGGGTAACGCCATCGCCGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAggcggc                                                                                                                           <  2:718596/151‑1 (MQ=255)
                 accacTGCGGGTAACGCCATCGCCGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGgcggcg                                                                                                                          >  2:602167/1‑151 (MQ=255)
                   cacTGCGGGTAACGCCATCGCCGGGAAATACACCAGCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGAc                                                                                                                        <  2:799944/151‑1 (MQ=255)
                           gTAACGCCATCGCCGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATcc                                                                                                                <  2:169126/151‑1 (MQ=255)
                             aaCGCCATCGCCGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCg                                                                                                               >  2:391075/1‑150 (MQ=255)
                                     cGCCGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCAtt                                                                                                      <  2:40263/151‑1 (MQ=255)
                                       ccGGGAAATACCCCATCAGCGTCACCCATCTGGCGCAGGCGCAATCCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTg                                                                                                     <  1:302205/150‑1 (MQ=255)
                                        cGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGat                                                                                                   >  2:799772/1‑151 (MQ=255)
                                        cGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGat                                                                                                   <  2:115009/151‑1 (MQ=255)
                                           gAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATc                                                                                                >  2:158569/1‑151 (MQ=255)
                                            aaaTACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCa                                                                                               <  2:206641/151‑1 (MQ=255)
                                              aTACACCATCATCGTCTGCCAGCTGGCGCAAGCGCAAACCCTGACCACGCGCACCCCCAGAGACAATGCGAAAACGGCGAGCGCCTCCAGCGACAGTACACCCACCATGCAAGAAGGCGGCGGCAAAGGTTCGTTTCCCATTGATATCGgc                                                                                             <  1:211512/151‑1 (MQ=255)
                                                acacCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAgcgc                                                                                           >  2:564443/1‑151 (MQ=255)
                                                   ccATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGc                                                                                        <  1:239499/151‑1 (MQ=255)
                                                         gCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTc                                                                                  <  2:325713/151‑1 (MQ=255)
                                                            tCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATc                                                                               >  1:237736/1‑151 (MQ=255)
                                                             cACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCg                                                                              <  1:444780/151‑1 (MQ=255)
                                                                 cATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTaa                                                                          <  2:678361/151‑1 (MQ=255)
                                                                 cATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTa                                                                           >  2:663391/1‑150 (MQ=255)
                                                                     tGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCgg                                                                      >  2:924254/1‑151 (MQ=255)
                                                                      ggcgcaggcgcaAACCCTGAACGCGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGTCAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAg                                                                         <  1:587056/147‑1 (MQ=255)
                                                                          caggcgcaAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATcc                                                                 >  1:928694/1‑151 (MQ=255)
                                                                            ggcgcaAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGACCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATcc                                                                 <  1:724043/149‑1 (MQ=255)
                                                                               gcaAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGAt                                                            <  2:112228/151‑1 (MQ=255)
                                                                                caAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATg                                                           <  2:701593/151‑1 (MQ=255)
                                                                                       tGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATca                                                     >  2:788374/1‑150 (MQ=255)
                                                                                        gACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATca                                                     <  2:145179/149‑1 (MQ=255)
                                                                                               cgcACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCa                                             >  1:917827/1‑150 (MQ=255)
                                                                                                  accaccAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCaaa                                           >  1:490073/1‑149 (MQ=255)
                                                                                                      ccaGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAgg                                     <  2:190870/151‑1 (MQ=255)
                                                                                                       caGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAGGc                                    <  2:235829/151‑1 (MQ=255)
                                                                                                         gagaCGATCCGAAAACGGCGATCGCCACCAGCGACAGTACCCTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGATAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAgg                                     <  1:285121/148‑1 (MQ=255)
                                                                                                                tACGAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAGGCGTAAgcgc                            >  1:827265/1‑150 (MQ=255)
                                                                                                                   gAAAACGGCGATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAGGCGTAAGCGCAAGc                        >  2:799649/1‑151 (MQ=255)
                                                                                                                            gATCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAGGCGTAAGCGCAAGCATCATTAAc               <  1:701971/151‑1 (MQ=255)
                                                                                                                              tCGCCACCAGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAGGCGTAAGCGCAAGCATCATTAACg              >  2:700462/1‑150 (MQ=255)
                                                                                                                                   accaGCGACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTa        <  2:188874/151‑1 (MQ=255)
                                                                                                                                   aacaGCCACAGTAAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCACCGCGGCTAACCCATCTTTAAGCGGGATCCGCGATGCCATCAACAACGCAAAAGCAGGCGTAAGCGCAAGCATCATTAACGTggg          >  1:58796/3‑149 (MQ=255)
                                                                                                                                         gACAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTg  <  2:813669/151‑1 (MQ=255)
                                                                                                                                          aCAGTACACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTg  >  2:287856/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                               
CCACCGCGTTCAGTGCCACCACTGCGGGTAACGCCATCGCCGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTG  >  NC_000913/2004107‑2004394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑