Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,056,488	A→C	R476R (CGA→CGC)	amn →	AMP nucleosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,056,488	0	A	C	100.0%	158.4 / NA	44	R476R (CGA→CGC)	amn	AMP nucleosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (24/20);  total (24/20)

CCGTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGAACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGAGTAAGTAAGACACCTGGCAAATAGCCTGCAATAATCGTGGGCTGTTTGCTTCCTTGGGCGGATACGAGTTTTATTATCG  >  NC_000913/2056348‑2056633                                                                                                                                             |                                                                                                                                                  ccGTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATg                                                                                                                                         >  2:644974/1‑151 (MQ=255)   gTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATg                                                                                                                                         >  2:831416/1‑149 (MQ=255)   gTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAg                                                                                                                                       <  1:454105/151‑1 (MQ=255)        aTGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAgccgcc                                                                                                                                  >  1:203767/1‑151 (MQ=255)         tGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGccgccg                                                                                                                                 >  2:824460/1‑151 (MQ=255)                  tAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGata                                                                                                                         >  2:143031/1‑150 (MQ=255)                         cccGGCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGtt                                                                                                                  <  2:608860/150‑1 (MQ=255)                            ggCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTg                                                                                                               >  2:73547/1‑150 (MQ=255)                                     aaCCGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACcttctt                                                                                                      >  2:540256/1‑150 (MQ=255)                                       ccGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATc                                                                                                   >  1:99370/1‑151 (MQ=255)                                       ccGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATc                                                                                                   <  1:658855/151‑1 (MQ=255)                                              tATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAAt                                                                                             >  1:163265/1‑150 (MQ=255)                                               aTGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATa                                                                                            <  1:236503/150‑1 (MQ=255)                                                     gcgcTATTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGa                                                                                     <  1:432050/151‑1 (MQ=255)                                                     gcgcTATTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGa                                                                                     <  1:195635/151‑1 (MQ=255)                                                          aTTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAgg                                                                                  <  1:344581/149‑1 (MQ=255)                                                          aTTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAgg                                                                                  <  2:34811/149‑1 (MQ=255)                                                          aTTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGag                                                                                <  1:496367/151‑1 (MQ=255)                                                           tttCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGagt                                                                               >  2:354003/1‑151 (MQ=255)                                                             tCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGagta                                                                              <  2:381002/150‑1 (MQ=255)                                                                 aaCATCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGagtaagta                                                                          >  1:786535/1‑150 (MQ=255)                                                                  aCATCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGagtaagta                                                                          >  1:254570/1‑149 (MQ=255)                                                                     tCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGAGTAAGTAAGac                                                                      >  1:627032/1‑150 (MQ=255)                                                                        gCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGAGTAAGTAAGac                                                                      <  2:491669/147‑1 (MQ=255)                                                                        gCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGAGTAAGTAAGACACCt                                                                  >  2:918219/1‑151 (MQ=255)                                                                          aaaTTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGAGTAAGTAAGACACCTgg                                                                >  1:190423/1‑151 (MQ=255)                                                                                gCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGAGTAAGTAAGACACCTGGCAAATa                                                          <  2:246357/151‑1 (MQ=255)                                                                                     cGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGAGTAAGTAAGACACCTGGCAAATAGCCt                                                      <  2:1072/150‑1 (MQ=255)                                                                                      gggCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGAGTAAGTAAGACACCTGGCAAATAGCCTGc                                                    <  1:619539/151‑1 (MQ=255)                                                                                      gggCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGAGTAAGTAAGACACCTGGCAAATAGCCTGc                                                    >  1:665127/1‑151 (MQ=255)                                                                                          gatcgatTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGAGTAAGTAAGACACCTGGCAAATAGCCTGCaat                                                 >  1:424877/1‑150 (MQ=255)                                                                                             cgatTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGAGTAAGTAAGACACCTGGCAAATAGCCTGCaataat                                              <  2:85391/150‑1 (MQ=255)                                                                                               atTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGAGTAAGTAAGACACCTGGCAAATAGCCTGCAATAATCGt                                           >  1:127559/1‑151 (MQ=255)                                                                                                     tgcGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGAGTAAGTAAGACACCTGGCAAATAGCCTGCAATAATCGTGGGCTg                                     >  1:371372/1‑151 (MQ=255)                                                                                                                ggCGACCGACTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGAGTAAGTAAGACACCTGGCAAATAGCCTGCAATAATCGTGGGCTGTTTGCTTCCtt                          >  2:33112/1‑151 (MQ=255)                                                                                                                   gaccgacTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGAGTAAGTAAGACACCTGGCAAATAGCCTGCAATAATCGTGGGCTGTTTGCTTCCTTggg                       <  1:478900/151‑1 (MQ=255)                                                                                                                    accgacTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGAGTAAGTAAGACACCTGGCAAATAGCCTGCAATAATCGTGGGCTGTTTGCTTCCTTGGGc                      >  2:669784/1‑151 (MQ=255)                                                                                                                    accgacTGCATTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGAGTAAGTAAGACACCTGGCAAATAGCCTGCAATAATCGTGGGCTGTTTGCTTCCTTGGGc                      >  2:832512/1‑151 (MQ=255)                                                                                                                             aTTCGCGTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGAGTAAGTAAGACACCTGGCAAATAGCCTGCAATAATCGTGGGCTGTTTGCTTCCTTGGGCGGATACg               >  1:89591/1‑149 (MQ=255)                                                                                                                                cgcgTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGAGTAAGTAAGACACCTGGCAAATAGCCTGCAATAATCGTGGGCTGTTTGCTTCCTTGGGCGGATACGAGttt          <  1:493401/151‑1 (MQ=255)                                                                                                                                  cgTAAATTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGAGTAAGTAAGACACCTGGCAAATAGCCTGCAATAATCGTGGGCTGTTTGCTTCCTTGGGCGGATACGAGtttt         >  2:485479/1‑150 (MQ=255)                                                                                                                                       aTTACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGAGTAAGTAAGACACCTGGCAAATAGCCTGCAATAATCGTGGGCTGTTTGCTTCCTTGGGCGGATACGAGTTTTATTATc   <  1:713633/151‑1 (MQ=255)                                                                                                                                         tACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGAGTAAGTAAGACACCTGGCAAATAGCCTGCAATAATCGTGGGCTGTTTGCTTCCTTGGGCGGATACGAGTTTTATTATCg  <  2:562742/150‑1 (MQ=255)                                                                                                                                         tACGCACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGAGTAAGTAAGACACCTGGCAAATAGCCTGCAATAATCGTGGGCTGTTTGCTTCCTTGGGCGGATACGAGTTTTATTATCg  <  1:695460/150‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                  CCGTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGACCGACTGCATTCGCGTAAATTACGAACCTTTAATGAGCCGCCGTTCCGATAATAAGTTCTGAACCTTCTTATCAGAAATAGATGTGAAGGAGTAAGTAAGACACCTGGCAAATAGCCTGCAATAATCGTGGGCTGTTTGCTTCCTTGGGCGGATACGAGTTTTATTATCG  >  NC_000913/2056348‑2056633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑