Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,083,952	T→G	V399V (GTT→GTG)	sbcB →	exodeoxyribonuclease I; exonuclease I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,083,952	0	T	G	100.0%	196.2 / NA	51	V399V (GTT→GTG)	sbcB	exodeoxyribonuclease I; exonuclease I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (22/29);  total (22/29)

GCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGTTATGCTGATGAATTGCA  >  NC_000913/2083809‑2084098                                                                                                                                                |                                                                                                                                                   gcgGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAAc                                                                                                                                             >  1:864857/1‑151 (MQ=255)   ggAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACgg                                                                                                                                           >  1:129172/1‑151 (MQ=255)     aaGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGAt                                                                                                                                         <  2:213386/151‑1 (MQ=255)        ccGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTACAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGa                                                                                                                                      <  2:245966/151‑1 (MQ=255)         cGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGaa                                                                                                                                     <  2:242096/151‑1 (MQ=255)          gAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGaa                                                                                                                                     >  2:807235/1‑150 (MQ=255)           aaCCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGaaaa                                                                                                                                   <  2:540708/151‑1 (MQ=255)           aaCCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGaaa                                                                                                                                    >  2:758815/1‑150 (MQ=255)              cGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCt                                                                                                                                >  2:623838/1‑151 (MQ=255)                   aCGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCtgttg                                                                                                                            <  2:846208/150‑1 (MQ=255)                         tCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCaa                                                                                                                       <  2:820028/149‑1 (MQ=255)                          cAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAAtt                                                                                                                     >  1:715743/1‑150 (MQ=255)                          cAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTa                                                                                                                    <  2:198052/151‑1 (MQ=255)                           aGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTAt                                                                                                                   >  1:484632/1‑151 (MQ=255)                                 cGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGc                                                                                                              >  1:656190/1‑150 (MQ=255)                                        gCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACt                                                                                                      <  1:786253/151‑1 (MQ=255)                                          acaGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCCCTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTc                                                                                                    <  1:732054/151‑1 (MQ=255)                                              cTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCgg                                                                                                >  1:777891/1‑151 (MQ=255)                                               tttATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCggg                                                                                               >  2:78712/1‑151 (MQ=255)                                                  ataACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGAc                                                                                            <  2:52940/151‑1 (MQ=255)                                                  ataACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGAc                                                                                            >  2:278912/1‑151 (MQ=255)                                                     aCGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCt                                                                                         >  1:889604/1‑151 (MQ=255)                                                              tCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATg                                                                                 <  1:907219/150‑1 (MQ=255)                                                              tCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGc                                                                                >  1:146423/1‑151 (MQ=255)                                                               cAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGcc                                                                               >  2:319307/1‑151 (MQ=255)                                                               cAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGc                                                                                <  2:746380/150‑1 (MQ=255)                                                                 gTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGa                                                                             <  2:183760/151‑1 (MQ=255)                                                                    aCGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGagca                                                                          <  2:47329/151‑1 (MQ=255)                                                                      gCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGagcagc                                                                        >  1:263295/1‑151 (MQ=255)                                                                         gATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAAc                                                                     <  2:822806/151‑1 (MQ=255)                                                                             gTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACgctg                                                                 <  2:871504/151‑1 (MQ=255)                                                                             gTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACgctg                                                                 <  2:430191/151‑1 (MQ=255)                                                                               gcagcaATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACgctggc                                                               >  1:115617/1‑151 (MQ=255)                                                                                           aTTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTc                                                   >  2:706867/1‑151 (MQ=255)                                                                                                 cTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAgg                                             >  1:614473/1‑151 (MQ=255)                                                                                                      aaCCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTc                                        <  1:461179/151‑1 (MQ=255)                                                                                                       aCCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTc                                        >  2:380244/1‑150 (MQ=255)                                                                                                       aCCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCa                                       <  2:316961/151‑1 (MQ=255)                                                                                                       aCCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCa                                       <  2:105323/151‑1 (MQ=255)                                                                                                         cGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTCAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACg                                     >  1:195021/1‑151 (MQ=255)                                                                                                                   aaTTTACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGtttt                           <  2:515611/151‑1 (MQ=255)                                                                                                                      ttACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTg                         <  2:738583/150‑1 (MQ=255)                                                                                                                       tACCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGc                        <  2:729220/150‑1 (MQ=255)                                                                                                                        aCCGGCACTGGATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGc                        >  2:130400/1‑149 (MQ=255)                                                                                                                                 ggATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGTTATg               <  2:164273/149‑1 (MQ=255)                                                                                                                                 ggATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGTTATGc              <  1:920923/150‑1 (MQ=255)                                                                                                                                  gATATCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGTTATGCtg            <  2:371230/151‑1 (MQ=255)                                                                                                                                     atCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGTTATGCtgatg         <  2:498111/151‑1 (MQ=255)                                                                                                                                     atCACTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGTTATGCtgatg         <  2:187094/151‑1 (MQ=255)                                                                                                                                        aCTTTTGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGTTATGCTGATGAAt      >  2:673071/1‑151 (MQ=255)                                                                                                                                           tttGTGGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGTTATGCTGATGAATTg    <  1:758796/150‑1 (MQ=255)                                                                                                                                             tgtggATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGTTATGCTGATGAATTGCa  <  2:864877/150‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                   GCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGTTATGCTGATGAATTGCA  >  NC_000913/2083809‑2084098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑