Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,107,808	T→G	R174S (AGA→AGC)	glf ←	UDP‑galactopyranose mutase, FAD/NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,107,808	0	T	G	100.0%	212.9 / NA	57	R174S (AGA→AGC)	glf	UDP‑galactopyranose mutase, FAD/NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (26/31);  total (26/31)

ACTCGCTAGAGAATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGT  >  NC_000913/2107664‑2107949                                                                                                                                                 |                                                                                                                                              aCTCGCTAGAGAATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACt                                                                                                                                         >  2:322650/1‑151 (MQ=255) aCTCGCTAGAGAATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCA‑TTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTg                                                                                                                                        >  2:316910/1‑151 (MQ=255)        agagAATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTc                                                                                                                                  <  1:339788/151‑1 (MQ=255)          agaATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGc                                                                                                                                >  1:645240/1‑151 (MQ=255)            aaTCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCtt                                                                                                                              >  2:112518/1‑151 (MQ=255)              tCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTta                                                                                                                             >  2:110890/1‑150 (MQ=255)                 ttGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTtaat                                                                                                                           <  2:802675/149‑1 (MQ=255)                 ttGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTta                                                                                                                             <  2:175327/147‑1 (MQ=255)                 ttGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTta                                                                                                                             <  2:138526/147‑1 (MQ=255)                   gTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTtaataat                                                                                                                        >  2:144463/1‑150 (MQ=255)                    tCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTtaataata                                                                                                                       >  1:859093/1‑150 (MQ=255)                    tCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTtaataata                                                                                                                       >  1:328261/1‑150 (MQ=255)                       ttCGAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATg                                                                                                                   >  2:429322/1‑151 (MQ=255)                       ttCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTtaataataa                                                                                                                      <  1:332586/148‑1 (MQ=255)                       ttCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATaaa                                                                                                                     <  1:926747/149‑1 (MQ=255)                       ttCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATaaa                                                                                                                     <  2:64253/149‑1 (MQ=255)                         cAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCa                                                                                                                 <  1:58041/151‑1 (MQ=255)                          aaaaaaTCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAg                                                                                                                <  1:136224/151‑1 (MQ=255)                           aaaaaTCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAgg                                                                                                               >  1:905870/1‑151 (MQ=255)                            aaaaTCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGc                                                                                                              <  2:613570/151‑1 (MQ=255)                             aaaTCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCa                                                                                                             <  1:160398/151‑1 (MQ=255)                                 cAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTc                                                                                                         >  1:788154/1‑151 (MQ=255)                                     gCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCtttt                                                                                                     >  2:574092/1‑151 (MQ=255)                                         aaTTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCa                                                                                                  <  1:433724/150‑1 (MQ=255)                                              tACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCActtctt                                                                                            >  2:145389/1‑151 (MQ=255)                                                cGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTcc                                                                                          >  2:393908/1‑151 (MQ=255)                                                    cacaCCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCAc                                                                                      >  2:752328/1‑151 (MQ=255)                                                     acacCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACt                                                                                     <  2:404435/151‑1 (MQ=255)                                                      cacCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTg                                                                                    >  2:493126/1‑151 (MQ=255)                                                      cacCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTg                                                                                    <  2:26818/151‑1 (MQ=255)                                                       acCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGc                                                                                   >  2:350884/1‑151 (MQ=255)                                                       acCTTCAAGCATTTTTTCAATAAGCTTAGTGCAGCCTCCCACCGGTATACCTTGATAGCGATCGGAAAAATAATTGTTATAAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGc                                                                                   >  1:749219/1‑151 (MQ=255)                                                        ccTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCt                                                                                  <  2:129170/151‑1 (MQ=255)                                                            aacgccttttttCAATAAGCTTAGTGTCGCCTCCCCCCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCCGGCAATTCTTTTGCACTTCTTCCCCACTGCTtctc                                                                              >  2:444209/7‑151 (MQ=255)                                                             aaGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTcc                                                                             <  1:90557/151‑1 (MQ=255)                                                                 aTTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGtat                                                                         >  1:335482/1‑151 (MQ=255)                                                                   tttttCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGtata                                                                        <  1:614666/150‑1 (MQ=255)                                                                    ttttCAATAAGCTTAGTGTAGCCACCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCTCTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATaa                                                                       <  1:299758/150‑1 (MQ=255)                                                                       tCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAAccc                                                                    <  2:179035/150‑1 (MQ=255)                                                                         aaTAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCttt                                                                 >  1:497948/1‑151 (MQ=255)                                                                         aaTAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCttt                                                                 >  2:464143/1‑151 (MQ=255)                                                                         aaTAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCttt                                                                 <  2:566195/151‑1 (MQ=255)                                                                          aTAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTa                                                                <  1:408769/151‑1 (MQ=255)                                                                                 tAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCaa                                                            <  1:109937/148‑1 (MQ=255)                                                                                 tAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATg                                                          <  1:488138/150‑1 (MQ=255)                                                                                 tAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATg                                                          <  1:883463/150‑1 (MQ=255)                                                                                         ccTCCCACCGGAATACCTTGATAGTGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGtat                                                  >  2:164252/1‑150 (MQ=255)                                                                                            cccACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATaa                                                <  1:748558/149‑1 (MQ=255)                                                                                                    aaTACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCa                                      <  2:490438/151‑1 (MQ=255)                                                                                                        ccTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTa                                  <  2:504716/151‑1 (MQ=255)                                                                                                           tGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAAt                                <  2:469972/150‑1 (MQ=255)                                                                                                            gATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGa                              <  2:239768/151‑1 (MQ=255)                                                                                                                 cGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAGTGAAATCg                         >  2:465953/1‑151 (MQ=255)                                                                                                                     cGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCt                      <  2:717101/150‑1 (MQ=255)                                                                                                                     cGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCt                      >  2:266221/1‑150 (MQ=255)                                                                                                                                       cAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAgg   <  2:768936/151‑1 (MQ=255)                                                                                                                                         aaCGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGt  >  2:866741/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                              ACTCGCTAGAGAATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGT  >  NC_000913/2107664‑2107949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑