Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,176,270	A→C	S18A (TCA→GCA)	gatZ ←	D‑tagatose 1,6‑bisphosphate aldolase 2, subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,176,270	0	A	C	100.0%	240.8 / NA	64	S18A (TCA→GCA)	gatZ	D‑tagatose 1,6‑bisphosphate aldolase 2, subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (26/38);  total (26/38)

ATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGTTATGCCCTGCCCTCGCAGCCACAATCGGCAATCACTTTGCTCACCACATCGCGCATTGCGGATTT  >  NC_000913/2176123‑2176415                                                                                                                                                    |                                                                                                                                                  aTTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCAc                                                                                                                                                 >  2:16095/1‑150 (MQ=255)    cgcgAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACatat                                                                                                                                             <  2:151765/151‑1 (MQ=255)    cgcgAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACatat                                                                                                                                             <  1:929911/151‑1 (MQ=255)    cgcgAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACatat                                                                                                                                             >  2:762427/1‑151 (MQ=255)    cgcgAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACat                                                                                                                                               <  1:128400/149‑1 (MQ=255)      cgAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGc                                                                                                                                           >  2:636843/1‑151 (MQ=255)      cgAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGc                                                                                                                                           <  2:163399/151‑1 (MQ=255)       gAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGcc                                                                                                                                          >  2:4789/1‑151 (MQ=255)       gAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGcc                                                                                                                                          >  1:455947/1‑151 (MQ=255)        aaaGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCg                                                                                                                                         <  2:82483/151‑1 (MQ=255)                       cATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGc                                                                                                                           <  2:365373/150‑1 (MQ=255)                            cGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATg                                                                                                                     <  2:224917/151‑1 (MQ=255)                             ggTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGc                                                                                                                    >  2:735185/1‑151 (MQ=255)                              gTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGcc                                                                                                                   >  1:930450/1‑151 (MQ=255)                                ataACCGCCAAATTGATTGACTTGGATTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGTCGCTTCGATAACCAACGGATGGGCAGAACAGACTCCACATATGCCGATATGTTCACCAGCTTTATGc                                                                                                                    >  1:428751/1‑148 (MQ=255)                                  aaCCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGc                                                                                                               <  1:82292/151‑1 (MQ=255)                                  aaCCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGc                                                                                                               <  2:30217/151‑1 (MQ=255)                                    ccGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGTCGGGCaa                                                                                                             <  2:504135/151‑1 (MQ=255)                                     cGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAAt                                                                                                            >  2:897043/1‑151 (MQ=255)                                     cGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAAt                                                                                                            >  2:576391/1‑151 (MQ=255)                                     cGCCAAAGTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAAt                                                                                                            <  1:192703/151‑1 (MQ=255)                                       ccAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTa                                                                                                          <  2:86910/151‑1 (MQ=255)                                       ccAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTa                                                                                                          <  2:809489/151‑1 (MQ=255)                                              gattgaCCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGtttt                                                                                                   <  1:776421/151‑1 (MQ=255)                                              gattgaCCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGttt                                                                                                    <  2:607411/150‑1 (MQ=255)                                               attgaCCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCCCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCCCCAGCTCTATGCCGTGCAATTAACGttta                                                                                                   <  1:314255/150‑2 (MQ=255)                                                 tgaCCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCAt                                                                                                >  1:283140/1‑151 (MQ=255)                                                  gaCCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATg                                                                                               <  1:24289/151‑1 (MQ=255)                                                     cTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGttt                                                                                            >  1:83227/1‑151 (MQ=255)                                                       ggTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGttttt                                                                                          >  2:454139/1‑151 (MQ=255)                                                       ggTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGtttt                                                                                           <  1:293595/150‑1 (MQ=255)                                                       ggTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATAtttt                                                                                           <  2:933711/150‑1 (MQ=255)                                                        gTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGtttttt                                                                                         >  1:604831/1‑151 (MQ=255)                                                         tttGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTc                                                                                        >  1:208093/1‑151 (MQ=255)                                                            gACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCtt                                                                                     <  1:915519/151‑1 (MQ=255)                                                            gACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCtt                                                                                     <  1:34310/151‑1 (MQ=255)                                                             aCGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTg                                                                                    <  2:625961/151‑1 (MQ=255)                                                             aCGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTg                                                                                    <  1:671446/151‑1 (MQ=255)                                                               gTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGtt                                                                                  >  2:110167/1‑151 (MQ=255)                                                               gTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGtt                                                                                  <  2:618413/151‑1 (MQ=255)                                                               gTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGtt                                                                                  <  2:329669/151‑1 (MQ=255)                                                                 tGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTa                                                                                 >  2:102434/1‑150 (MQ=255)                                                                  gCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTaa                                                                                <  2:446001/150‑1 (MQ=255)                                                                   cTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAAt                                                                              >  1:611732/1‑151 (MQ=255)                                                                     tCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAAt                                                                              <  2:643908/149‑1 (MQ=255)                                                                     tCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATg                                                                             <  2:397503/150‑1 (MQ=255)                                                                        aTCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAg                                                                         <  1:870461/151‑1 (MQ=255)                                                                          cAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGt                                                                       <  1:59028/151‑1 (MQ=255)                                                                           aGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTg                                                                      >  2:453930/1‑151 (MQ=255)                                                                           aGCACTTTGCGAGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTg                                                                      >  1:811885/1‑151 (MQ=255)                                                                                      cgTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGTTAtgccct                                                           <  2:655640/151‑1 (MQ=255)                                                                                           tGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGTTAtgccctgcc                                                        >  2:180641/1‑149 (MQ=255)                                                                                              tGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGTTATGCCCTGCCCTCg                                                    <  1:416166/150‑1 (MQ=255)                                                                                                 gATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGTTATGCCCTGCCCTCGCAGc                                                >  2:178902/1‑151 (MQ=255)                                                                                                  aTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGTTATGCCCTGCCCTCGCAGcc                                               >  1:761731/1‑151 (MQ=255)                                                                                                                                 ggATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGTTATGCCCTGCCCTCGCAGCCACAATCGGCAATCACTTTGCTCACCACATcg                <  1:177219/151‑1 (MQ=255)                                                                                                                                 ggATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGTTATGCCCTGCCCTCGCAGCCACAATCGGCAATCACTTTGCTCACCACATcg                <  1:106019/151‑1 (MQ=255)                                                                                                                                  gATGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGTTATGCCCTGCCCTCGCAGCCACAATCGGCAATCACTTTGCTCACCACATcgc               <  1:599358/151‑1 (MQ=255)                                                                                                                                   aTGGGCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGTTATGCCCTGCCCTCGCAGCCACAATCGGCAATCACTTTGCTCACCACATcgcg              <  1:209326/151‑1 (MQ=255)                                                                                                                                     tggCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGTTATGCCCTGCCCTCGCAGCCACAATCGGCAATCACTTTGCTCACCACATCgcgc             >  1:513574/2‑150 (MQ=255)                                                                                                                                     gggCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTGGTTAAATGGCAGGTGCGTTATGCCCTGCCCTCGCAGCCACAATCGGCAATCACTTTGCTCACCACATcgcg              <  1:159056/149‑1 (MQ=255)                                                                                                                                      ggCAGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGTTATGCCCTGCCCTCGCAGCCACAATCGGCAATCACTTTGCTCACCACATCGCGCa            <  2:544090/150‑1 (MQ=255)                                                                                                                                         aGAACAGACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGTTATGCCCTGCCCTCGCAGCCACAATCGGCAATCACTTTGCTCACCACATCGCGCATTGc        >  1:63452/1‑151 (MQ=255)                                                                                                                                               gACTGCACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGTTATGCCCTGCCCTCGCAGCCACAATCGGCAATCACTTTGCTCACCACATCGCGCATTGCGGAttt  >  2:277308/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                  ATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGTTATGCCCTGCCCTCGCAGCCACAATCGGCAATCACTTTGCTCACCACATCGCGCATTGCGGATTT  >  NC_000913/2176123‑2176415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑