Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,214,569	T→G	K26Q (AAA→CAA)	yehU ←	inner membrane putative sensory kinase in two‑component system with YehT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,214,569	0	T	G	100.0%	185.2 / NA	52	K26Q (AAA→CAA)	yehU	inner membrane putative sensory kinase in two‑component system with YehT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (28/24);  total (28/24)

CCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTTCTTTGAACTCTTGC  >  NC_000913/2214421‑2214716                                                                                                                                                     |                                                                                                                                                    ccTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGAc                                                                                                                                                   <  1:752408/151‑1 (MQ=255)      cGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCAtt                                                                                                                                              >  1:769746/1‑151 (MQ=255)        cACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTaa                                                                                                                                            <  1:33522/151‑1 (MQ=255)           gggTATTGGCAATAGAATCGTCAATGTGCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCa                                                                                                                                         <  2:518038/151‑1 (MQ=255)               aTTGGCAATAGAATCGTCAATGTGCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCa                                                                                                                                     <  2:464480/151‑1 (MQ=255)                 tGGCAATAGAATCGTCAATGTGCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCaa                                                                                                                                    <  2:450530/150‑1 (MQ=255)                   gCAATAGAATCGTCAATGTGCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATg                                                                                                                                  >  1:678626/1‑150 (MQ=255)                   gCAATAGAATCGTCAATGTGCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATg                                                                                                                                  >  1:484247/1‑150 (MQ=255)                        aGAATCGTCAATGTGCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTaaa                                                                                                                            <  2:458378/151‑1 (MQ=255)                        aGAATCGTCAATGTGCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTaaa                                                                                                                            <  2:567452/151‑1 (MQ=255)                         gAATCGTCAATGTGCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTaaaa                                                                                                                           <  1:468331/151‑1 (MQ=255)                           aTCGTCAATGTGCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTaaaaaa                                                                                                                         <  1:396413/151‑1 (MQ=255)                             cGTCAATGTGCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAAc                                                                                                                       >  2:681277/1‑151 (MQ=255)                                cAATGTGCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGc                                                                                                                     >  2:378982/1‑150 (MQ=255)                                 aaTGTGCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGcac                                                                                                                   >  1:375537/1‑151 (MQ=255)                                      gCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATctg                                                                                                              <  2:563528/151‑1 (MQ=255)                                             aaaCCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAagc                                                                                                       >  1:610636/1‑151 (MQ=255)                                                    gTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAagcagcagc                                                                                                 >  2:837469/1‑150 (MQ=255)                                                     tGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGATGAAgcagcagca                                                                                                >  2:818557/1‑150 (MQ=255)                                                      gCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAac                                                                                              >  2:78061/1‑151 (MQ=255)                                                               gCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGAtt                                                                                      >  2:372289/1‑150 (MQ=255)                                                                   aagaTGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAAt                                                                                 <  1:136205/151‑1 (MQ=255)                                                                    agaTGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAGTGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATc                                                                                >  1:830783/1‑151 (MQ=255)                                                                    agaTGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATc                                                                                >  2:783690/1‑151 (MQ=255)                                                                        ggAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATAGTa                                                                             <  1:746932/150‑1 (MQ=255)                                                                         gAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACGCCAGATTAAAATCGTACa                                                                           <  1:186823/151‑1 (MQ=255)                                                                               aCGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCg                                                                     <  2:930750/151‑1 (MQ=255)                                                                                  aTGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCaa                                                                  <  1:335437/151‑1 (MQ=255)                                                                                    gTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCaaa                                                                 >  1:411510/1‑150 (MQ=255)                                                                                       gCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACcac                                                             >  1:297585/1‑151 (MQ=255)                                                                                         agagAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCacac                                                           >  1:405011/1‑151 (MQ=255)                                                                                          gagaAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAAGACCAGATTAAAATCGTACATACCCGCAAACcaca                                                            >  1:352651/1‑149 (MQ=255)                                                                                           agaAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACtt                                                         <  2:604699/151‑1 (MQ=255)                                                                                           agaAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACtt                                                         <  1:796186/151‑1 (MQ=255)                                                                                              aaTTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTccc                                                      <  2:8162/151‑1 (MQ=255)                                                                                               aTTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCt                                                     >  1:536674/1‑151 (MQ=255)                                                                                                 ttATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCttt                                                   >  1:913995/1‑151 (MQ=255)                                                                                                   aTGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTa                                                  <  1:329872/150‑1 (MQ=255)                                                                                                          aGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGt                                          >  2:180108/1‑151 (MQ=255)                                                                                                              gAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGACGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAac                                       >  2:20642/1‑150 (MQ=255)                                                                                                                aCCGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAacat                                     >  1:574451/1‑150 (MQ=255)                                                                                                                  cGTGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAacatac                                   >  1:94411/1‑150 (MQ=255)                                                                                                                    tGACCTGCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACAtt                                >  2:130198/1‑151 (MQ=255)                                                                                                                          gCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCt                            <  2:826742/149‑1 (MQ=255)                                                                                                                          gCATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTg                           <  1:919159/150‑1 (MQ=255)                                                                                                                           cATTAACGGTATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGc                          >  2:667451/1‑150 (MQ=255)                                                                                                                                    tATGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAActtt                >  1:382124/1‑151 (MQ=255)                                                                                                                                     aTGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAActttc               <  2:434504/151‑1 (MQ=255)                                                                                                                                     aTGAATAATGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTACTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAActttc               <  2:274677/151‑1 (MQ=255)                                                                                                                                        aataatGGCGTTTGACTCATTAACCATGCAATGACTAAAAAGACGCACATCTGCTGAAGCAGCAGCGGCTCCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAGCATACGTTGCCTGCGTTTAActttcttt            <  1:237493/151‑1 (MQ=255)                                                                                                                                            atGGCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTTCTTTGAAc        >  1:79591/1‑151 (MQ=255)                                                                                                                                               gCGTTTGACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTTCTTTGAActct     >  1:842736/1‑151 (MQ=255)                                                                                                                                                  tttgACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTTCTTTGAACTCTTGc  >  2:256898/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    CCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTTCTTTGAACTCTTGC  >  NC_000913/2214421‑2214716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑