Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,266,579	T→G	I112S (ATT→AGT)	yeiQ →	putative NAD‑dependent D‑mannonate oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,266,579	0	T	G	100.0%	239.5 / NA	64	I112S (ATT→AGT)	yeiQ	putative NAD‑dependent D‑mannonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (24/40);  total (24/40)

TTCAGCGGTGATCAACTGATGAGCCAGCTCCGCGCACAGAACCATTTATATACCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTATTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCGGTGCACTCGACACCAGTAATCCGCGGATTATTCACGATCTACAAACCCCTGAAGAACCTCACTCCGC  >  NC_000913/2266431‑2266726                                                                                                                                                     |                                                                                                                                                    ttCAGCGGTGATCAACTGATGAGCCAGCTCCGCGCACAGAACCATTTATATACCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTg                                                                                                                                                   >  1:270593/1‑151 (MQ=255)    aGCGGTGATCAACTGATGAGCCAGCTCCGCGCACAGAACCATTTATATACCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTg                                                                                                                                                   >  2:934779/1‑148 (MQ=255)    aGCGGTGATCAACTGATGAGCCAGCTCCGCGCACAGAACCATTTATATACCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTg                                                                                                                                                   >  2:553418/1‑148 (MQ=255)    aGCGGTGATCAACTGATGAGCCAGCTCCGCGCACAGAACCATTTATATACCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTg                                                                                                                                                   >  1:512151/1‑148 (MQ=255)      cGGTGATCAACTGATGAGCCAGCTCCGCGCACAGAACCATTTATATACCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGaaa                                                                                                                                              <  1:366496/151‑1 (MQ=255)      cGGTGATCAACTGATGAGCCAGCTCCGCGCACAGAACCATTTATATACCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGaaa                                                                                                                                              <  1:37231/151‑1 (MQ=255)          gATCAACTGATGAGCCAGCTCCGCGCACAGAACCATTTATATACCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAAtttt                                                                                                                                          >  2:710342/1‑151 (MQ=255)           aTCAACTGATGAGCCAGCTCCGCGCACAGAACCATTTATATACCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTg                                                                                                                                         >  2:637365/1‑151 (MQ=255)           aTCAACTGATGAGCCAGCTCCGCGCACAGAACCATTTATATACCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTg                                                                                                                                         <  2:579634/151‑1 (MQ=255)             cAACTGATGAGCCAGCTCCGCGCACAGAACCATTTATATACCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGc                                                                                                                                        <  1:590348/150‑1 (MQ=255)                 tgatgaGCCAGCTCCGCGCACAGAACCATTTATATACCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGcc                                                                                                                                   >  2:451544/1‑151 (MQ=255)                       gCCAGCTCCGCGCACAGAACCATTTATATACCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAgg                                                                                                                              <  1:272601/150‑1 (MQ=255)                        ccAGCTCCGCGCACAGAACCATTTATATACCGTGCTGGAGAAAGGGGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGGGAGAAATTTTGCGAGCcac                                                                                                                                 >  2:237346/1‑146 (MQ=255)                          aGCTCCGCGCACAGAACCATTTATATACCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGc                                                                                                                          >  1:146940/1‑151 (MQ=255)                               cgcgCACAGAACCATTTATATACCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTg                                                                                                                     <  1:214734/151‑1 (MQ=255)                                 cgcACAGAACCATTTATATACCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGtt                                                                                                                   >  2:207529/1‑151 (MQ=255)                                 cgcACAGAACCATTTATATACCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGtt                                                                                                                   >  1:183141/1‑151 (MQ=255)                                  gcACAGAACCATTTATATACCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGttt                                                                                                                  >  1:429532/1‑151 (MQ=255)                                  gcACAGAACCATTTATATACCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGttt                                                                                                                  <  2:374680/151‑1 (MQ=255)                                  gcACAGAACCATTTATATACCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGttt                                                                                                                  >  1:271699/1‑151 (MQ=255)                                      aGAACCATTTATATACCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCt                                                                                                              >  2:910339/1‑151 (MQ=255)                                       gAACCATTTATATACCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTg                                                                                                             <  2:518439/151‑1 (MQ=255)                                        aaCCATTTATATACCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGa                                                                                                            <  2:159164/151‑1 (MQ=255)                                          ccATTTATATACCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACg                                                                                                          <  2:488983/151‑1 (MQ=255)                                           cATTTATATACCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATGCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGa                                                                                                         <  1:5101/151‑1 (MQ=255)                                               tatataCCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTa                                                                                                      <  2:333381/150‑1 (MQ=255)                                               tatataCCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTa                                                                                                      <  1:861725/150‑1 (MQ=255)                                                  ataCCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCg                                                                                                   <  1:605661/150‑1 (MQ=255)                                                  ataCCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGCCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCg                                                                                                   <  2:702018/150‑1 (MQ=255)                                                      cGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGaaaaa                                                                                              <  2:672413/151‑1 (MQ=255)                                                        tGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAg                                                                                             <  2:695074/150‑1 (MQ=255)                                                          cTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCt                                                                                          >  1:46071/1‑151 (MQ=255)                                                                   ggTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCtattgtattg                                                                                 <  1:364972/151‑1 (MQ=255)                                                                   ggTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCtattgtattg                                                                                 <  2:280081/151‑1 (MQ=255)                                                                        ggACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCg                                                                            >  2:13888/1‑151 (MQ=255)                                                                            ggCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGcc                                                                         >  1:334240/1‑150 (MQ=255)                                                                            ggCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCa                                                                        <  1:846280/151‑1 (MQ=255)                                                                              cAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCAcc                                                                      <  1:615421/151‑1 (MQ=255)                                                                               aaTCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCg                                                                     >  1:646449/1‑151 (MQ=255)                                                                               aaTCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCg                                                                     <  1:421650/151‑1 (MQ=255)                                                                                  cAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCGGt                                                                   <  1:933976/150‑1 (MQ=255)                                                                                        aTAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGTCGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCGGTGCACTCg                                                            >  1:219661/1‑151 (MQ=255)                                                                                          aaTTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCGGTGCACTCGac                                                          <  2:643309/151‑1 (MQ=255)                                                                                              gTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCGGTGCACTCGACAcc                                                       <  1:304623/150‑1 (MQ=255)                                                                                                           aCGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCGGTGCACTCGACACCAGTAATCCGCGGat                                         <  2:756909/151‑1 (MQ=255)                                                                                                           aCGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCGGTGCACTCGACACCAGTAATCCGCGGat                                         >  2:147359/1‑151 (MQ=255)                                                                                                             gAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCGGTGCACTCGACACCAGTAATCCGCGGatta                                       <  1:936026/151‑1 (MQ=255)                                                                                                                 gCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCGGTGCACTCGACACCAGTAATCCGCGGATTATTCa                                   <  1:877144/151‑1 (MQ=255)                                                                                                                 gCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCGGTGCACTCGACACCAGTAATCCGCGGATTATTCa                                   <  1:905927/151‑1 (MQ=255)                                                                                                                  ccTTAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCGGTGCACTCGACACCAGTAATCCGCGGATTATTCAc                                  <  2:161871/151‑1 (MQ=255)                                                                                                                    ttAATGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCGGTGCACTCGACACCAGTAATCCGCGGATTATTCACg                                 >  2:263115/1‑150 (MQ=255)                                                                                                                       aTGCAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCGGTGCACTCGACACCAGTAATCCGCGGATTATTCACGATCt                             <  2:428640/151‑1 (MQ=255)                                                                                                                          cAAAACTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCGGTGCACTCGACACCAGTAATCCGCGGATTATTCACGATCTACa                          <  1:633620/151‑1 (MQ=255)                                                                                                                            aaaCTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCGGTGCACTCGACACCAGTAATCCGCGGATTATTCACGATCTACaaa                        <  2:875331/151‑1 (MQ=255)                                                                                                                               cTGGATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCGGTGCACTCGACACCAGTAATCCGCGGATTATTCACGATCTACAAAccc                     >  1:594300/1‑151 (MQ=255)                                                                                                                                 ggATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCGGTGCACTCGACACCAGTAATCCGCGGATTATTCACGATCTACAAAccc                     <  2:594450/149‑1 (MQ=255)                                                                                                                                 ggATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCGGTGCACTCGACACCAGTAATCCGCGGATTATTCACGATCTACAAAccc                     <  2:141582/149‑1 (MQ=255)                                                                                                                                 ggATTCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCGGTGCACTCGACACCAGTAATCCGCGGATTATTCACGATCTACAAACCCCt                   <  1:63649/151‑1 (MQ=255)                                                                                                                                     tCCTTAGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCGGTGCACTCGACACCAGTAATCCGCGGATTATTCACGATCTACAAACCCCTga                 >  2:617422/1‑149 (MQ=255)                                                                                                                                          aGCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCGGTGCACTCGACACCAGTAATCCGCGGATTATTCACGATCTACAAACCCCTGAAGAAcc           <  2:419541/150‑1 (MQ=255)                                                                                                                                           gCGGCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCGGTGCACTCGACACCAGTAATCCGCGGATTATTCACGATCTACAAACCCCTGAAGAACCTc         <  2:646469/151‑1 (MQ=255)                                                                                                                                             ggCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCGGTGCACTCTACACCAGTAATCCGCGGATTATTCACGATCTACAAACCCCTGAAGAACCTc         <  1:128367/149‑1 (MQ=255)                                                                                                                                             ggCAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCGGTGCACTCGACACCAGTAATCCGCGGATTATTCACGATCTACAAACCCCTGAAGAACCTCAc       >  1:834857/1‑151 (MQ=255)                                                                                                                                               cAATTAGTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCGGTGCACTCGACACCAGTAATCCGCGGATTATTCACGATCTACAAACCCCTGAAGAACCTCACTc     <  1:676552/151‑1 (MQ=255)                                                                                                                                                  ttagtGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCGGTGCACTCGACACCAGTAATCCGCGGATTATTCACGATCTACAAACCCCTGAAGAACCTCACTCCGc  >  1:832675/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    TTCAGCGGTGATCAACTGATGAGCCAGCTCCGCGCACAGAACCATTTATATACCGTGCTGGAGAAAGGTGCGGACGGCAATCAGGTGATAATTGTCGGTGCCGTTCACGAATGCCTTAATGCAAAACTGGATTCCTTAGCGGCAATTATTGAGAAATTTTGCGAGCCACAGGTGGCAATTGTTTCCCTGACGATTACCGAAAAAGGCTATTGTATTGACCCGGCCACCGGTGCACTCGACACCAGTAATCCGCGGATTATTCACGATCTACAAACCCCTGAAGAACCTCACTCCGC  >  NC_000913/2266431‑2266726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑