Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,283,668	T→G	K94Q (AAA→CAA)	yejK ←	spermidine nucleoid‑associated protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,283,668	0	T	G	100.0%	156.2 / NA	42	K94Q (AAA→CAA)	yejK	spermidine nucleoid‑associated protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (22/20);  total (22/20)

TCAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGTTAACGCGCATACTGCTCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACCC  >  NC_000913/2283523‑2283814                                                                                                                                                  |                                                                                                                                                   tCAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGTTAACGCGCATACTGCTCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCt                                                                                                                                                >  1:716152/1‑150 (MQ=255)  cAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGTTAACGCGCATACTGCTCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTaa                                                                                                                                              <  1:18239/151‑1 (MQ=255)         aTGGGTTGGGTTGATGTCGAGATTTTCGTTAACGCGCATACTGCTCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTc                                                                                                                                        >  1:542650/1‑150 (MQ=255)           gggttgggttGATGTCGAGATTTTCGTTAACGCGCATACTGCTCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACg                                                                                                                                     <  1:606467/151‑1 (MQ=255)            ggttgggttgATGTCGAGATTTTCGTTAACGCGCATACTGCTCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGc                                                                                                                                    >  2:11346/1‑151 (MQ=255)               tgggttgATGTCGAGATTTTCGTTAACGCGCATACTGCTCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAgg                                                                                                                                 >  2:138450/1‑151 (MQ=255)                        gTCGAGATTTTCGTTAACGCGCATACTGCTCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGtt                                                                                                                        <  1:500459/151‑1 (MQ=255)                          cGAGATTTTCGTTAACGCGCATACTGCTCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGc                                                                                                                      <  2:545470/151‑1 (MQ=255)                              aTTTTCGTTAACGCGCATACTGCTCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCcgcg                                                                                                                  >  1:305395/1‑151 (MQ=255)                                tttCGTTAACGCGCATACTGCTCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCcgcgc                                                                                                                 <  1:191479/150‑1 (MQ=255)                                tttCGTTAACGCGCATACTGCTCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCcgcgc                                                                                                                 <  2:89686/150‑1 (MQ=255)                                          gcgcATACTGCTCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGcc                                                                                                      <  1:87204/151‑1 (MQ=255)                                            gcATACTGCTCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGcc                                                                                                      >  2:209212/1‑149 (MQ=255)                                              aTACTGCTCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAgg                                                                                                   >  1:539102/1‑150 (MQ=255)                                               tACTGCTCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGa                                                                                                  >  1:368930/1‑150 (MQ=255)                                                aCTGCTCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAg                                                                                                <  2:758751/151‑1 (MQ=255)                                                 cTGCTCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAg                                                                                                >  2:587215/1‑150 (MQ=255)                                                       aGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACTCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCctc                                                                                           <  1:334772/149‑1 (MQ=255)                                                         gTTGCTCAGAACTGCCACCAGCAAATACTCCACCACCAGATAGCGATAGTGGCAAAACAGCACGAATCAACCGTCAGCAAAAGGATATTGCGATAATACGTCACACAGGCGGCCGGTTGCCGCGCGGCTAAATGCCGGGAAGTCCTCTTc                                                                                        <  1:220072/150‑1 (MQ=255)                                                               cAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTg                                                                                  >  2:88233/1‑150 (MQ=255)                                                                    cTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTg                                                                            >  1:676583/1‑151 (MQ=255)                                                                     tGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGt                                                                           >  2:441298/1‑151 (MQ=255)                                                                      gCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGt                                                                           >  2:933354/1‑150 (MQ=255)                                                                       ccaccaGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTaa                                                                         <  2:475183/151‑1 (MQ=255)                                                                                 aTACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTc                                                               <  2:188312/151‑1 (MQ=255)                                                                                    cTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTg                                                             <  1:872692/150‑1 (MQ=255)                                                                                       cACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGcc                                                         >  1:412517/1‑151 (MQ=255)                                                                                        aCCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGcc                                                         >  2:380427/1‑150 (MQ=255)                                                                                            ccAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTc                                                    >  1:887066/1‑151 (MQ=255)                                                                                             cAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCa                                                   <  1:434334/151‑1 (MQ=255)                                                                                                 tAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCActct                                               >  2:800560/1‑151 (MQ=255)                                                                                                    cGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCtt                                            <  1:543434/151‑1 (MQ=255)                                                                                                           ggCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTaaa                                     <  2:350719/151‑1 (MQ=255)                                                                                                                      aCGAATCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTa                            >  2:806500/1‑149 (MQ=255)                                                                                                                         aaTCCACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCtt                       >  2:638538/1‑151 (MQ=255)                                                                                                                             cACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTTTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAtt                   <  1:8002/151‑1 (MQ=255)                                                                                                                             cACCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAtt                   <  2:365583/151‑1 (MQ=255)                                                                                                                              aCCGTCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAttt                  >  1:676073/1‑151 (MQ=255)                                                                                                                                  tCAGCAAAAGGATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTgg              >  2:749937/1‑151 (MQ=255)                                                                                                                                           ggATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGa     <  2:735558/151‑1 (MQ=255)                                                                                                                                            gATATTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGAc    >  1:485945/1‑151 (MQ=255)                                                                                                                                               atTGCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGAccc  <  2:748483/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                   TCAAGATAATGGGTTGGGTTGATGTCGAGATTTTCGTTAACGCGCATACTGCTCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACCC  >  NC_000913/2283523‑2283814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑