Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,316,351	A→C	M59L (ATG→CTG)	rcsB →	response regulator in two‑component regulatory system with RcsC and YojN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,316,351	0	A	C	100.0%	187.9 / NA	53	M59L (ATG→CTG)	rcsB	response regulator in two‑component regulatory system with RcsC and YojN
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (27/26);  total (27/26)

CGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTG  >  NC_000913/2316203‑2316497                                                                                                                                                     |                                                                                                                                                   cGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTg                                                                                                                                                  <  1:772906/151‑1 (MQ=255)     gACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCcctgcctg                                                                                                                                              >  2:156099/1‑151 (MQ=255)         aTCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCg                                                                                                                                           >  2:433640/1‑150 (MQ=255)         aTCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGa                                                                                                                                          <  1:547100/151‑1 (MQ=255)            cGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATa                                                                                                                                        >  1:359305/1‑150 (MQ=255)                aGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTAc                                                                                                                                   <  1:10989/151‑1 (MQ=255)                               tCGCAAATCACTTGAGCAAATTGAGTGGGAGAATGTTGTCGGCGACTTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTAcc                                                                                                                    >  2:295579/1‑151 (MQ=255)                                      tCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATc                                                                                                              >  2:294976/1‑150 (MQ=255)                                        aCTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCa                                                                                                             >  2:396846/1‑149 (MQ=255)                                                 aaTTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCa                                                                                                    >  1:614231/1‑149 (MQ=255)                                                  aTTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCACTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAgc                                                                                                 <  1:674866/151‑1 (MQ=255)                                                           gTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTcc                                                                                        <  2:313650/151‑1 (MQ=255)                                                             gAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTccc                                                                                       <  1:711369/150‑1 (MQ=255)                                                              aaTGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCa                                                                                      >  1:756024/1‑150 (MQ=255)                                                               aTGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAg                                                                                    <  2:400116/151‑1 (MQ=255)                                                                 gttgtCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGcc                                                                                  >  2:437354/1‑151 (MQ=255)                                                                 gttgtCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGcc                                                                                  <  1:27021/151‑1 (MQ=255)                                                                 gttgtCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGcc                                                                                  >  1:300615/1‑151 (MQ=255)                                                                 gttgtCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGcc                                                                                  >  2:37956/1‑151 (MQ=255)                                                                    gtCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTg                                                                                <  2:280523/150‑1 (MQ=255)                                                                       ggCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGAT‑GCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGAt                                                                           <  2:501281/151‑1 (MQ=255)                                                                             tttGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTg                                                                      >  1:638356/1‑151 (MQ=255)                                                                                 aaGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCt                                                                  <  1:689376/151‑1 (MQ=255)                                                                                  aGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTg                                                                 <  2:740331/151‑1 (MQ=255)                                                                                         aCAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATg                                                           >  2:883248/1‑150 (MQ=255)                                                                                          cAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGa                                                          <  1:397335/150‑1 (MQ=255)                                                                                             cACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGaaca                                                       >  2:898233/1‑150 (MQ=255)                                                                                                   tCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAAccc                                                >  1:58110/1‑151 (MQ=255)                                                                                                    caacaaCCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAAccc                                                <  2:741197/150‑1 (MQ=255)                                                                                                     aacaacCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCgg                                              >  2:393673/1‑151 (MQ=255)                                                                                                     aacaacCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCgg                                              <  2:355231/151‑1 (MQ=255)                                                                                                      acaacCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGc                                             <  1:890878/151‑1 (MQ=255)                                                                                                          ccTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGTCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACATCCCGGCGAtt                                         <  1:904386/151‑1 (MQ=255)                                                                                                          ccTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGAtt                                         <  2:364179/151‑1 (MQ=255)                                                                                                            tGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTc                                        >  1:361143/1‑150 (MQ=255)                                                                                                              ccGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTa                                     <  2:626504/151‑1 (MQ=255)                                                                                                                 aaaCTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTg                                  <  1:780224/151‑1 (MQ=255)                                                                                                                 aaaCTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTg                                  >  1:505569/1‑151 (MQ=255)                                                                                                                   aCTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCg                                <  1:758980/151‑1 (MQ=255)                                                                                                                   aCTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCg                                <  1:434151/151‑1 (MQ=255)                                                                                                                   aCTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCg                                <  1:313470/151‑1 (MQ=255)                                                                                                                     tGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGt                              >  2:209975/1‑151 (MQ=255)                                                                                                                      ggATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCgg                               <  2:868597/149‑1 (MQ=255)                                                                                                                           cgcATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTgg                         >  2:876758/1‑150 (MQ=255)                                                                                                                            gcATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGAt                       >  1:379791/1‑151 (MQ=255)                                                                                                                            gcATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGAt                       >  2:572120/1‑151 (MQ=255)                                                                                                                                  gtTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGat                 >  1:616290/1‑151 (MQ=255)                                                                                                                                   ttGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGat                 >  2:393048/1‑150 (MQ=255)                                                                                                                                        tACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGa            <  1:240940/150‑1 (MQ=255)                                                                                                                                        tACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGa            >  1:198718/1‑150 (MQ=255)                                                                                                                                            gATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAggg        >  2:267934/1‑150 (MQ=255)                                                                                                                                            gATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTACTGGATCTGGATATCGAAg          >  1:366695/1‑148 (MQ=255)                                                                                                                                                 ctcCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATc     <  2:50602/148‑1 (MQ=255)                                                                                                                                                 ctcCCTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTg  >  1:40645/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTG  >  NC_000913/2316203‑2316497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑