Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,438,570 A→C P207P (CCA→CCC)  flk → putative flagella assembly protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,438,5700AC98.2% 191.2 / ‑3.6 55P207P (CCA→CCC) flkputative flagella assembly protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (32/22);  total (32/23)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01

AGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTAGATGCCAGTGAACTGGCGGCGTTATCGGCATATATCCA  >  NC_000913/2438422‑2438716
                                                                                                                                                    |                                                                                                                                                  
aGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGc                                                                                                                                                  >  2:378670/1‑151 (MQ=255)
    gaccgaccGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGaa                                                                                                                                               >  2:196897/1‑150 (MQ=255)
     accgaccgTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAAc                                                                                                                                             >  2:726324/1‑151 (MQ=255)
      ccgaccgTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAAc                                                                                                                                             <  2:588141/150‑1 (MQ=255)
       cgaccgTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTg                                                                                                                                           >  1:116073/1‑151 (MQ=255)
          ccgTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGttt                                                                                                                                        >  2:457687/1‑151 (MQ=255)
                tttATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCAt                                                                                                                                  >  2:272677/1‑151 (MQ=255)
                 ttattaCCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCAt                                                                                                                                  <  1:122570/150‑1 (MQ=255)
                      aCCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGAGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCt                                                                                                                                >  1:894500/1‑147 (MQ=255)
                         ggcggAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGAcc                                                                                                                         <  2:784810/151‑1 (MQ=255)
                         ggcggAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGAcc                                                                                                                         >  2:777575/1‑151 (MQ=255)
                          gcggAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCt                                                                                                                        <  1:494114/151‑1 (MQ=255)
                            ggAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGCCCTg                                                                                                                       >  2:157418/1‑150 (MQ=255)
                             gAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGc                                                                                                                     >  2:354096/1‑151 (MQ=255)
                                cacaATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTAc                                                                                                                  >  2:432836/1‑151 (MQ=255)
                                cacaATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTAc                                                                                                                  <  1:800564/151‑1 (MQ=255)
                                 acaATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACa                                                                                                                 <  2:78656/151‑1 (MQ=255)
                                  caATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACa                                                                                                                 <  1:662890/150‑1 (MQ=255)
                                              aCAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGAc                                                                                                     >  1:293012/1‑150 (MQ=255)
                                               cAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGc                                                                                                   >  2:375046/1‑151 (MQ=255)
                                                           aaaCTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCaa                                                                                        >  1:820138/1‑150 (MQ=255)
                                                            aaCTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCaaa                                                                                       >  2:862310/1‑150 (MQ=255)
                                                                tGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATa                                                                                    >  1:706840/1‑149 (MQ=255)
                                                                  cggcggCAAC‑GGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCg                                                                               <  2:234843/151‑1 (MQ=255)
                                                                    gcggcAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGa                                                                              <  1:738098/151‑1 (MQ=255)
                                                                                gAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCAc                                                                  <  2:383659/151‑1 (MQ=255)
                                                                                     cAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAg                                                             <  1:510283/151‑1 (MQ=255)
                                                                                            cAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGc                                                      >  2:190356/1‑151 (MQ=255)
                                                                                            cAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGc                                                      >  2:118048/1‑151 (MQ=255)
                                                                                                tCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTaa                                                   >  2:709509/1‑150 (MQ=255)
                                                                                                      aaTCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAAcc                                            >  1:277709/1‑151 (MQ=255)
                                                                                                           aTGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCttt                                         >  2:836438/1‑149 (MQ=255)
                                                                                                               tGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTAGATGc                                   >  1:886983/1‑151 (MQ=255)
                                                                                                                ggAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTAGATg                                    <  2:428416/149‑1 (MQ=255)
                                                                                                                       tCCGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTAGATGCCAGTGAAc                           >  2:462721/1‑151 (MQ=255)
                                                                                                                        ccGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTAGATGCCAGTGAACt                          >  2:852070/1‑151 (MQ=255)
                                                                                                                        ccGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTAGATGCCAGTGAACt                          <  2:593502/151‑1 (MQ=255)
                                                                                                                         cGGGGTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTAGATGCCAGTGAACTg                         >  1:626035/1‑151 (MQ=255)
                                                                                                                           gggTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTAGATGCCAGTGAACTggc                       <  2:674990/151‑1 (MQ=255)
                                                                                                                           gggTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTAGATGCCAGTGAACTgg                        <  2:376055/150‑1 (MQ=255)
                                                                                                                             gTGAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTAGATGCCAGTGAACTggcgg                     <  1:443882/151‑1 (MQ=255)
                                                                                                                               gAAAGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTAGATGCCAGTGAACTGgcggcg                   >  2:576137/1‑151 (MQ=255)
                                                                                                                                 aaGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTAGATGCCAGTGAACTGGCGGCGtt                 >  2:542004/1‑151 (MQ=255)
                                                                                                                                  aGATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTAGATGCCAGTGAACTGGCGGCGtt                 >  2:354200/1‑150 (MQ=255)
                                                                                                                                   gATGGCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTAGATGCCAGTGAACTGGCGGCGTTAt               >  2:269491/1‑151 (MQ=255)
                                                                                                                                    aTGGCGAGTTAACTCCCGCGAAACTCTTTAACCACCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACATATGACGCTAAAACAACCTCTAGAGGCCAGTGAACTGGCGGCGTTAt               <  1:604506/150‑1 (MQ=255)
                                                                                                                                       gCGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTAGATGCCAGTGAACTGGCGGCGTTATCGGc           <  2:742524/151‑1 (MQ=255)
                                                                                                                                        cGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTAGATGCCAGTGAACTGGCGGCGTTATCGGc           >  2:482911/1‑150 (MQ=255)
                                                                                                                                        cGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTAGATGCCAGTGAACTGGCGGCGTTATCGGc           >  2:636024/1‑150 (MQ=255)
                                                                                                                                        cGAGTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTAGATGCCAGTGAACTGGCGGCGTTATCGGc           >  1:253031/1‑150 (MQ=255)
                                                                                                                                           gTTAATTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTAGATGCCAGTGAACTGGCGGCGTTATCGGCatat       <  1:462882/151‑1 (MQ=255)
                                                                                                                                              aaTTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTAGATGCCAGTGAACTGGCGGCGTTATCGGCatatat     <  1:34218/150‑1 (MQ=255)
                                                                                                                                               aTTCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTAGATGCCAGTGAACTGGCGGCGTTATCGGCATATATcc   <  2:9325/151‑1 (MQ=255)
                                                                                                                                                 tCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTAGATGCCAGTGAACTGGCGGCGTTATCGGCATATATCCa  <  2:216781/150‑1 (MQ=255)
                                                                                                                                                 tCCCGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTAGATGCCAGTGAACTGGCGGCGTTATCGGCATATATCCa  <  1:743351/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTAGATGCCAGTGAACTGGCGGCGTTATCGGCATATATCCA  >  NC_000913/2438422‑2438716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑