Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,486,868	T→G	V832G (GTC→GGC)	evgS →	hybrid sensory histidine kinase in two‑component regulatory system with EvgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,486,868	0	T	G	100.0%	194.3 / NA	54	V832G (GTC→GGC)	evgS	hybrid sensory histidine kinase in two‑component regulatory system with EvgA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (32/22);  total (32/22)

CCACAATGGGTCGATATCCCTACTTTAGTCCAGAACACTTGTCACTCTTTCGGTGCGATTGCTGCAAGCAAATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGTCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTGGGTCACATTGATGACAACCACGCTGTTATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACA  >  NC_000913/2486720‑2487015                                                                                                                                                     |                                                                                                                                                    ccACAATGGGTCGATATCCCTACTTTAGTCCAGAACACTTGTCACTCTTTCGGTGCGATTGCTGCAAGCAAATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCt                                                                                                                                                   >  1:624460/1‑151 (MQ=255)  cacaATGGGTCGATATCCCTACTTTAGTCCAGAACACTTGTCACTCTTTCGGTGCGATTGCTGCAAGCAAATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCtt                                                                                                                                                  <  2:475869/151‑1 (MQ=255)    caATGGGTCGATATCCCTACTTTAGTCCAGAACACTTGTCACTCTTTCGGTGCGATTGCTGCAAGCAAATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTa                                                                                                                                                 <  1:479823/150‑1 (MQ=255)        gggTCGATATCCCTACTTTAGTCCAGAACACTTGTCACTCTTTCGGTGCGATTGCTGCAAGCAAATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCaaa                                                                                                                                            <  1:880513/151‑1 (MQ=255)           tCGATATCCCTACTTTAGTCCAGAACACTTGTCACTCTTTCGGTGCGATTGCTGCAAGCAAATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAAttt                                                                                                                                         >  2:441371/1‑151 (MQ=255)                 tCCCTACTTTAGTCCAGAACACTTGTCACTCTTTCGGTGCGATTGCTGCAAGCAAATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTg                                                                                                                                     >  2:533709/1‑149 (MQ=255)                 tCCCTACTTTAGTCCAGAACACTTGTCACTCTTTCGGTGCGATTGCTGCAAGCAAATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTg                                                                                                                                     >  2:106056/1‑149 (MQ=255)                      aCTTTAGTCCAGAACACTTGTCACTCTTTCGGTGCGATTGCTGCAAGCAAATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATg                                                                                                                              >  2:884456/1‑151 (MQ=255)                            gTCCAGAACACTTGTCACTCTTTCGGTGCGATTGCTGCAAGCAAATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCtctc                                                                                                                         >  1:664647/1‑150 (MQ=255)                             tCCAGCACACTTGTCACTCTTTCGGTGCGATTGCTGCAAGCAAATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCtctc                                                                                                                         >  1:123611/1‑149 (MQ=255)                                  aaCACTTGTCACTCTTTCGGTGCGATTGCTGCAAGCAAATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTa                                                                                                                  <  1:555954/151‑1 (MQ=255)                                        tGTCACTCTTTCGGTGCGATTGCTGCAAGCAAATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCg                                                                                                            >  1:58493/1‑151 (MQ=255)                                           cACTCTTTCGGTGCGATTGCTGCAAGCAAATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAgg                                                                                                         >  1:257789/1‑151 (MQ=255)                                            aCTCTTTCGGTGCGATTGCTGCAAGCAAATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAggg                                                                                                        >  2:541420/1‑151 (MQ=255)                                               ctTTCGGTGCGATTGCTGCAAGCAAATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAgggg                                                                                                       <  1:125215/149‑1 (MQ=255)                                                tttCGGTGCGATTGCTGCAAGCAAATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGc                                                                                                     >  1:563526/1‑150 (MQ=255)                                                 ttCGGTGCGATTGCTGCAAGCAAATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAggggg                                                                                                      <  2:484039/148‑1 (MQ=255)                                                      tGCGATTGCTGCAAGCAAATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTaaa                                                                                               >  2:91747/1‑150 (MQ=255)                                                        cGATTGCTGCAAGCAAATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAAt                                                                                            >  1:57392/1‑151 (MQ=255)                                                        cGATTGCTGCAAGCAAATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAAt                                                                                            >  1:27877/1‑151 (MQ=255)                                                         gATTGCTGCAAGCAAATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAAtt                                                                                           >  2:635352/1‑151 (MQ=255)                                                             gctgcAAGCAAATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACg                                                                                        >  1:323713/1‑150 (MQ=255)                                                                gcaagcaaATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGAcc                                                                                     <  2:378940/150‑1 (MQ=255)                                                                gcaagcaaATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGAc                                                                                      <  2:701714/149‑1 (MQ=255)                                                                    gcaaATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCt                                                                                <  2:205241/151‑1 (MQ=255)                                                                     caaATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTg                                                                               <  1:724227/151‑1 (MQ=255)                                                                     caaATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTg                                                                               >  1:423088/1‑151 (MQ=255)                                                                      aaaTCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTgg                                                                              <  2:34059/151‑1 (MQ=255)                                                                       aaTCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTggg                                                                             >  1:640352/1‑151 (MQ=255)                                                                              cgCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTGGGTCACAtt                                                                      >  2:394450/1‑151 (MQ=255)                                                                               gCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTGGGTCACATtg                                                                     >  1:588956/1‑151 (MQ=255)                                                                                cATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTGGGTCACATtg                                                                     >  1:326330/1‑150 (MQ=255)                                                                                    aaGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGGCCTCCCTGGGTCACATTGATGAc                                                                <  2:531831/151‑1 (MQ=255)                                                                                                          gAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTGGGTCACATTGATGACAACCACGCTGTTATCAAAAt                                            <  2:851707/149‑1 (MQ=255)                                                                                                            aCATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTGGGTCACATTGATGACAACCACGCTGTTATCAAAATGACg                                        <  1:12895/151‑1 (MQ=255)                                                                                                            aCATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTGGGTCACATTGATGACAACCACGCTGTTATCAAAATGACg                                        <  1:400960/151‑1 (MQ=255)                                                                                                             cATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTGGGTCACATTGATGACAACCACGCTGTTATCAAAATGACg                                        >  2:368382/1‑150 (MQ=255)                                                                                                                 aCCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTGGGTCACATTGATGACAACCACGCTGTTATCAAAATGACGATTAt                                   >  1:491129/1‑151 (MQ=255)                                                                                                                   cTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTGGGTCACATTGATGACAACCACGCTGTTATCAAAATGACGATTATgg                                 >  2:114472/1‑151 (MQ=255)                                                                                                                   cTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTGGGTCACATTGATGACAACCACGCTGTTATCAAAATGACGATTATgg                                 >  2:737710/1‑151 (MQ=255)                                                                                                                      gTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTGGGTCACATTGATGACAACCACGCTGTTATCAAAATGACGATTATGGAtt                              <  1:493942/151‑1 (MQ=255)                                                                                                                      gTTAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTGGGTCACATTGATGACAACCACGCTGTTATCAAAATGACGATTATGGAtt                              <  1:467062/151‑1 (MQ=255)                                                                                                                        tAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTGGGTCACATTGATGACAACCACGCTGTTATCAAAATGACGATTATGGATTCt                            >  1:885444/1‑151 (MQ=255)                                                                                                                        tAAGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTGGGTCACATTGATGACAACCACGCTGTTATCAAAATGACGATTATGGATTCt                            >  2:425025/1‑151 (MQ=255)                                                                                                                         aaGATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTGGGTCACATTGATGACAACCACGCTGTTATCAAAATGACGATTATGGATTCt                            <  1:666673/150‑1 (MQ=255)                                                                                                                           gATCGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTGGGTCACATTGATGACAACCACGCTGTTATCAAAATGACGATTATGGATTCTgg                          >  2:573920/1‑150 (MQ=255)                                                                                                                              cGACCCTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTGGGTCACATTGATGACAACCACGCTGTTATCAAAATGACGATTATGGATTCTGGAAGt                      >  2:128844/1‑151 (MQ=255)                                                                                                                                 cccTCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTGGGTCACATTGATGACAACCACGCTGTTATCAAAATGACGATTATGGATTCTGGAAGTGGa                   <  1:230585/151‑1 (MQ=255)                                                                                                                                    tCAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTGGGTCACATTGATGACAACCACGCTGTTATCAAAATGACGATTATGGATTCTGGAAGTGGAtt                 >  2:665718/1‑150 (MQ=255)                                                                                                                                     cAGGCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTGGGTCACATTGATGACAACCACGCTGTTATCAAAATGACGATTATGGATTCTGGAAGTGGAtt                 <  2:130200/149‑1 (MQ=255)                                                                                                                                       ggCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTGGGTCACATTGATGACAACCACGCTGTTATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCg             >  1:569587/1‑151 (MQ=255)                                                                                                                                       ggCGTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTGGGTCACATTGATGACAACCACGCTGTTATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCg             <  2:142629/151‑1 (MQ=255)                                                                                                                                          gTTTAAGCAGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTGGGTCACATTGATGACAACCACGCTGTTATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCGc            >  2:869418/1‑149 (MQ=255)                                                                                                                                                  aGGGCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTGGGTCACATTGATGACAACCACGCTGTTATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGaaca  <  1:63698/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    CCACAATGGGTCGATATCCCTACTTTAGTCCAGAACACTTGTCACTCTTTCGGTGCGATTGCTGCAAGCAAATCGATCGCATTAAGTTGCAGCAGTACGTTTCCTGAACATTACCTGGTTAAGATCGACCCTCAGGCGTTTAAGCAGGTCTTATCAAATTTACTGAGTAATGCTCTCAAATTTACCACCGAGGGGGCAGTAAAAATTACGACCTCCCTGGGTCACATTGATGACAACCACGCTGTTATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACA  >  NC_000913/2486720‑2487015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑