Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,554,262	T→G	Q307H (CAA→CAC)	eutR ←	eut operon transcriptional activator, AraC family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,554,262	0	T	G	100.0%	182.3 / NA	49	Q307H (CAA→CAC)	eutR	eut operon transcriptional activator, AraC family
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (21/28);  total (21/28)

GTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGACATGCAGTTGATTACACAAATCCAGCACC  >  NC_000913/2554119‑2554409                                                                                                                                                |                                                                                                                                                    gtgtAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGAcc                                                                                                                                              <  2:393291/151‑1 (MQ=255)   gtAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCAc                                                                                                                                            >  1:758415/1‑151 (MQ=255)            tCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATc                                                                                                                                   >  1:73719/1‑151 (MQ=255)            tCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATc                                                                                                                                   >  1:558062/1‑151 (MQ=255)                ccccACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGtt                                                                                                                               <  1:157349/151‑1 (MQ=255)                        ccGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGt                                                                                                                       >  1:223041/1‑151 (MQ=255)                        ccGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGt                                                                                                                       >  1:64074/1‑151 (MQ=255)                        ccGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCAAGGACTTATCAGTTCGCGGCGt                                                                                                                       <  2:524857/151‑1 (MQ=255)                         cGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGt                                                                                                                       >  2:434943/1‑150 (MQ=255)                          gCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTAc                                                                                                                     >  2:308183/1‑151 (MQ=255)                            aTCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACgg                                                                                                                   >  1:735027/1‑151 (MQ=255)                              ccGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCg                                                                                                                 >  1:918605/1‑151 (MQ=255)                              ccGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCg                                                                                                                 >  1:681418/1‑151 (MQ=255)                                    aTGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAg                                                                                                            <  1:167401/150‑1 (MQ=255)                                      gCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCg                                                                                                         <  1:725011/151‑1 (MQ=255)                                        aGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGaa                                                                                                       <  1:474457/151‑1 (MQ=255)                                         gCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAAt                                                                                                      >  2:5651/1‑151 (MQ=255)                                          cGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATg                                                                                                     <  2:77422/151‑1 (MQ=255)                                               gTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGttt                                                                                                <  2:320279/151‑1 (MQ=255)                                               gTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGttt                                                                                                >  2:323480/1‑151 (MQ=255)                                                   cGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAg                                                                                             <  2:754340/150‑1 (MQ=255)                                                   cGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGc                                                                                            <  2:236088/151‑1 (MQ=255)                                                    ggCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGcc                                                                                           <  2:85743/151‑1 (MQ=255)                                                     gCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCa                                                                                          <  1:332609/151‑1 (MQ=255)                                                        tctcGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCAc                                                                                         <  2:403799/149‑1 (MQ=255)                                                        tctcGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCAc                                                                                         <  2:55989/149‑1 (MQ=255)                                                         ctcGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCAcgcg                                                                                      >  1:124339/1‑151 (MQ=255)                                                           cGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCAcgcg                                                                                      <  1:512705/149‑1 (MQ=255)                                                            ggAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTc                                                                                   <  2:721321/151‑1 (MQ=255)                                                              aaaaCAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCgg                                                                                 >  1:872541/1‑151 (MQ=255)                                                              aaaaCAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCgg                                                                                 <  1:378258/151‑1 (MQ=255)                                                                  cAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGcc                                                                              >  2:452066/1‑150 (MQ=255)                                                                              aTCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATa                                                                 <  1:599754/151‑1 (MQ=255)                                                                                 cGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCg                                                              >  2:678478/1‑151 (MQ=255)                                                                                    ggCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTg                                                            <  1:16782/150‑1 (MQ=255)                                                                                       aaaTTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGaaa                                                         <  1:835675/150‑1 (MQ=255)                                                                                                 aGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTa                                              <  2:538002/151‑1 (MQ=255)                                                                                                 aGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTa                                              <  1:458857/151‑1 (MQ=255)                                                                                                    tGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTC‑GGCCAATGCCCAAAAAAGCGTGAAACGCGTTTTggaacgg                                          >  1:388240/1‑145 (MQ=255)                                                                                                          aaTCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTgcggc                                     <  2:296933/151‑1 (MQ=255)                                                                                                          aaTCCCCACGGCATGGCCGCGTCTTTTACCGTGATACTGTGCGACCAGGGACTAATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTATCAGCCACGCGTTCGGGGCAATGCATAAAATAGCGTGAAACGCGTTTTGTAGCGAgcggc                                     <  1:297721/151‑1 (MQ=255)                                                                                                             ccccACTGCATGGCGGCGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGcggcgg                                   <  1:387538/150‑1 (MQ=255)                                                                                                                        ggcggcGTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGACATGCAg                        <  1:809320/150‑1 (MQ=255)                                                                                                                           ggcgTCTTTTACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGACATGCAGTTg                     >  1:439060/1‑150 (MQ=255)                                                                                                                                 ttttACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGACATGCAGTTGATTacac              >  1:85152/1‑151 (MQ=255)                                                                                                                                   ttACCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGACATGCAGTTGATTAcaca             >  2:930780/1‑150 (MQ=255)                                                                                                                                     aCCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGACATGCAGTTGATTACACAAAt          <  2:810339/151‑1 (MQ=255)                                                                                                                                     aCCGTCATACTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGACATGCAGTTGATTACACAAAt          <  1:192364/151‑1 (MQ=255)                                                                                                                                             aCTGTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGACATGCAGTTGATTACACAAATCCAGCAcc  >  1:427479/1‑151 (MQ=255)                                                                                                                                                |                                                                                                                                                    GTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGACATGCAGTTGATTACACAAATCCAGCACC  >  NC_000913/2554119‑2554409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑