Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,578,624 A→C intergenic (‑247/‑42) maeB ← / → talA malic enzyme: putative oxidoreductase/phosphotransacetylase/transaldolase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,578,6240AC100.0% 210.6 / NA 59intergenic (‑247/‑42)maeB/talAmalic enzyme: putative oxidoreductase/phosphotransacetylase/transaldolase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (31/28);  total (31/28)

CTTTGAGCAAGTCCAAACTCTCACCATTAATATAATGTTTTGGTAATAATCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTAACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAACAGTTCACCACTGTCGTGGCAGACAGCGGCGATATTGAGTCCATTCGCCATTATCATCCCCAGGATGCCACCACCAATCC  >  NC_000913/2578477‑2578769
                                                                                                                                                   |                                                                                                                                                 
cTTTGAGCAAGTCCAAACTCTCACCATTAATATAATGTTTTGGTAATAATCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACg                                                                                                                                                >  2:774770/1‑151 (MQ=255)
    gAGCAAGTCCAAACTCTCACCATTAATATAATGTTTTGGTAATAATCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACt                                                                                                                                            <  2:914127/151‑1 (MQ=255)
       cAAGTCCAAACTCTCACCATTAATATAATGTTTTGGTAATAATCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCAt                                                                                                                                         <  1:143061/151‑1 (MQ=255)
       cAAGTCCAAACTCTCACCATTAATATAATGTTTTGGTAATAATCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCAt                                                                                                                                         >  2:543359/1‑151 (MQ=255)
        aaGTCCAAACTCTCACCATTAATATAATGTTTTGGTAATAATCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATc                                                                                                                                        >  1:725269/1‑151 (MQ=255)
        aaGTCCAAACTCTCACCATTAATATAAGGTTTTGGTAATAATCCTATAACGCTGATGTTACCTGCTTAATCCATCGATGCGATGTCTGTCTGCTATACTTATTTGAGCAGTTTAGCGAAGTTTCTCAGATGTGTGAATTCACACTCTCGTc                                                                                                                                        <  1:451872/151‑1 (MQ=255)
         aGTCCAAACTCTCACCATTAATATAATGTTTTGGTAATAATCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCt                                                                                                                                       <  1:222471/151‑1 (MQ=255)
               aaCTCTCACCATTAATATAATGTTTTGGTAATAATCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACact                                                                                                                                 >  2:148455/1‑151 (MQ=255)
                 ctctCACCATTAATATAATGTTTTGGTAATAATCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACacttt                                                                                                                               >  2:806070/1‑151 (MQ=255)
                 ctctCACCATTAATATAATGTTTTGGTAATAATCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACacttt                                                                                                                               >  1:80578/1‑151 (MQ=255)
                    tcACCATTAATATAATGTTTTGGTAATAATCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACactttact                                                                                                                            >  1:103417/1‑151 (MQ=255)
                     cACCATTAATATAATGTTTTGGTAATAATCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACacttta                                                                                                                              <  1:190002/148‑1 (MQ=255)
                      aCCATTAATATAATGTTTTGGTAATAATCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCAGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACactttacttt                                                                                                                          <  2:923300/151‑1 (MQ=255)
                       ccATTAATATAATGTTTTGGTAATAATCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACactttacttt                                                                                                                          <  1:558811/150‑1 (MQ=255)
                       ccATTAATATAATGTTTTGGTAATAATCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACactttacttt                                                                                                                          <  1:445172/150‑1 (MQ=255)
                       ccATTAATATAATGTTTTGGTAATAATCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACtttt                                                                                                                         <  2:909817/151‑1 (MQ=255)
                         aTTAATATAATGTTTTGGTAATAATCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCa                                                                                                                       <  1:803141/151‑1 (MQ=255)
                         aTTAATATAATGTTTTGGTAATAATCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCa                                                                                                                       <  2:323432/151‑1 (MQ=255)
                           tAATATAATGTTTTGGTAATAATCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCaa                                                                                                                      <  1:462331/150‑1 (MQ=255)
                               ataATGTTTTGGTAATAATCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGt                                                                                                                 <  2:820121/151‑1 (MQ=255)
                                    gTTTTGGTAATAATCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTAttt                                                                                                             <  1:816782/150‑1 (MQ=255)
                                    gTTTTGGTAATAATCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTc                                                                                                            >  1:86430/1‑151 (MQ=255)
                                        tGGTAATAATCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTAt                                                                                                        >  1:467015/1‑151 (MQ=255)
                                         ggTAATAATCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTAt                                                                                                        <  1:369678/150‑1 (MQ=255)
                                         ggTAATAATCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATg                                                                                                       >  2:736004/1‑151 (MQ=255)
                                           taataaTCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGa                                                                                                      >  2:199513/1‑150 (MQ=255)
                                           taataaTCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGa                                                                                                      >  1:80797/1‑150 (MQ=255)
                                           taataaTCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGa                                                                                                      >  1:729780/1‑150 (MQ=255)
                                               aatCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAg                                                                                                 <  2:352428/151‑1 (MQ=255)
                                                atCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGt                                                                                                <  1:229139/151‑1 (MQ=255)
                                                             gATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATc                                                                                     <  1:457098/149‑1 (MQ=255)
                                                                 ttACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAAc                                                                                 >  1:286825/1‑149 (MQ=255)
                                                                    ccTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAACAGTTc                                                                            <  2:168227/151‑1 (MQ=255)
                                                                     cTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAACAGTTc                                                                            >  1:263640/1‑150 (MQ=255)
                                                                      tGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAACAGTTcac                                                                          >  1:124971/1‑151 (MQ=255)
                                                                       gCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAACAGTTcacc                                                                         >  1:859160/1‑151 (MQ=255)
                                                                            aTCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAACAGTTCACCACTGt                                                                    >  1:887736/1‑151 (MQ=255)
                                                                              ccAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAACAGTTCACCACTGTc                                                                   <  2:613830/150‑1 (MQ=255)
                                                                                      aCCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAACAGTTCACCACTGTCGTGGCAGAc                                                          >  2:524427/1‑151 (MQ=255)
                                                                                        cctGCCCGTCTGCTATTGTTTTTTGGATCGTTTAGGGGAATTTCTTAAAAATGTTAAATCCCCGACTCCTCTAACCCCTTTCTTTTCAAAGAAGAATTCCTTTGGACCAGTTAGACGGCATCAAACAGTTCACCACTGTCGTGGCAGACAg                                                        <  2:322405/149‑1 (MQ=255)
                                                                                                tCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAACAGTTCACCACTGTCGTGGCAGACAGCGGCGat                                                 >  2:804493/1‑150 (MQ=255)
                                                                                                 cTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAACAGTTCACCACTGTCGTGGCAGACAGCGGCGatat                                               >  2:826635/1‑151 (MQ=255)
                                                                                                   gCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAACAGTTCACCACTGTCGTGGCAGACAGCGGCGATATTg                                             <  2:348389/151‑1 (MQ=255)
                                                                                                      aTGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAACAGTTCACCACTGTCGTGGCAGACAGCGGCGATATTGAGt                                          <  1:94481/151‑1 (MQ=255)
                                                                                                                   gCGTTTAGCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAACAGTTCACCACTGTCGTGGCAGACAGCGGCGATATTGAGTCCATTCGCCAtt                              <  2:834532/150‑1 (MQ=255)
                                                                                                                          gCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAACAGTTCACCACTGTCGTGGCAGACAGCGGCGATATTGAGTCCATTCGCCATTATCATccc                      <  2:223481/151‑1 (MQ=255)
                                                                                                                          gCGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAACAGTTCACCACTGTCGTGGCAGACAGCGGCGATATTGAGTCCATTCGCCATTATCATccc                      >  2:589647/1‑151 (MQ=255)
                                                                                                                           cGAAATTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAACAGTTCACCACTGTCGTGGCAGACAGCGGCGATATTGAGTCCATTCGCCATTATCATcccc                     <  1:316878/151‑1 (MQ=255)
                                                                                                                             aaaTTTCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAACAGTTCACCACTGTCGTGGCAGACAGCGGCGATATTGAGTCCATTCGCCATTATCATcccc                     >  1:382137/1‑149 (MQ=255)
                                                                                                                                tttCTCAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAACAGTTCACCACTGTCGTGGCAGACAGCGGCGATATTGAGTCCATTCGCCATTATCATCCCCAGGAt                >  1:107413/1‑151 (MQ=255)
                                                                                                                                   ctcAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAACAGTTCACCACTGTCGTGGCAGACAGCGGCGATATTGAGTCCATTCGCCATTATCATCCCCAGGATGcc             >  1:678604/1‑151 (MQ=255)
                                                                                                                                     cAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAACAGTTCACCACTGTCGTGGCAGACAGCGGCGATATTGAGTCCATTCGCCATTATCATCCCCAGGATGccac           >  1:275926/1‑151 (MQ=255)
                                                                                                                                     cAGAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAACAGTTCACCACTGTCGTGGCAGACAGCGGCGATATTGAGTCCATTCGCCATTATCATCCCCAGGATGc              <  1:588549/148‑1 (MQ=255)
                                                                                                                                       gAAGTGTGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAACAGTTCACCACTGTCGTGGCAGACAGCGGCGATATTGAGTCCATTCGCCATTATCATCCCCAGGATGccacc          >  2:232972/1‑150 (MQ=255)
                                                                                                                                          gtgtGAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAACAGTTCACCACTGTCGTGGCAGACAGCGGCGATATTGAGTCCATTCGCCATTATCATCCCCAGGATGccaccacca      <  1:424940/151‑1 (MQ=255)
                                                                                                                                            gtgAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAACAGTTCACCACTGTCGTGGCAGACAGCGGCGATATTGAGTCCATTCGCCATTATCATCCCCAGGATGCCACCACCAAt    >  2:27101/1‑151 (MQ=255)
                                                                                                                                            gtgAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAACAGTTCACCACTGTCGTGGCAGACAGCGGCGATATTGAGTCCATTCGCCATTATCATCCCCAGGATGCCACCACCAAt    >  1:620759/1‑151 (MQ=255)
                                                                                                                                             tgAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAACAGTTCACCACTGTCGTGGCAGACAGCGGCGATATTGAGTCCATTCGCCATTATCATCCCCAGGATGCCACCACCAATc   >  1:719553/1‑151 (MQ=255)
                                                                                                                                              gAATTCACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAACAGTTCACCACTGTCGTGGCAGACAGCGGCGATATTGAGTCCATTCGCCATTATCATCCCCAGGATGCCACCACCAATcc  >  1:429877/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CTTTGAGCAAGTCCAAACTCTCACCATTAATATAATGTTTTGGTAATAATCCTATAACACTGATGTTACCTGCTTAATCCAGCAATACCATGCCTGTCTGCTATGCTTTTTTGATGCGTTTAGCGAAATTTCTCAGAAGTGTGAATTAACGCACTCATCTAACACTTTACTTTTCAAGGAGTATTTCCTATGAACGAGTTAGACGGCATCAAACAGTTCACCACTGTCGTGGCAGACAGCGGCGATATTGAGTCCATTCGCCATTATCATCCCCAGGATGCCACCACCAATCC  >  NC_000913/2578477‑2578769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: