Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,594,162	A→C	D566E (GAT→GAG)	tmcA ←	elongator methionine tRNA (ac4C34) acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,594,162	0	A	C	100.0%	223.0 / NA	60	D566E (GAT→GAG)	tmcA	elongator methionine tRNA (ac4C34) acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (24/36);  total (24/36)

ACGCCCACGCAGCGCCGGTAATGCCAGTTCACTGGTTTGTAACAGACGCAATAAGCAACCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCC  >  NC_000913/2594015‑2594307                                                                                                                                                     |                                                                                                                                                  aCGCCCACGCAGCGCCGGTAATGCCAGTTCACTGGTTTGTAACAGACGCAATAAGCAACCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCa                                                                                                                                                <  1:448300/151‑1 (MQ=255)    cccACGCAGCGCCGGTAATGCCAGTTCACTGGTTTGTAACAGACGCAATAAGCAACCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACa                                                                                                                                             <  1:244005/151‑1 (MQ=255)        cGCAGCGCCGGTAATGCCAGTTCACTGGTTTGTAACAGACGCAATAAGCAACCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGa                                                                                                                                          >  2:615668/1‑150 (MQ=255)        cGCAGCGCCGGTAATGCCAGTTCACTGGTTTGTAACAGACGCAATAAGCAACCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGa                                                                                                                                          >  2:317762/1‑150 (MQ=255)            gcgcCGGTAATGCCAGTTCACTGGTTTGTAACAGACGCAATAAGCAACCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCg                                                                                                                                      <  1:444042/150‑1 (MQ=255)              gcCGGTAATGCCAGTTCACTGGTTTGTAACAGACGCAATAAGCAACCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGttt                                                                                                                                   <  1:409882/151‑1 (MQ=255)                cGGTAATGCCAGTTCACTGGTTTGTAACAGACGCAATAAGCAACCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCg                                                                                                                                 >  2:673044/1‑151 (MQ=255)                 ggTAATGCCAGTTCACTGGTTTGTAACAGACGCAATAAGCAACCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGc                                                                                                                                <  1:214936/151‑1 (MQ=255)                    aaTGCCAGTTCACTGGTTTGTAACAGACGCAATAAGCAACCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCAt                                                                                                                             <  2:375434/151‑1 (MQ=255)                    aaTGCCAGTTCACTGGTTTGTAACAGACGCAATAAGCAACCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCAt                                                                                                                             <  2:899556/151‑1 (MQ=255)                    aaTGCCAGTTCACTGGTTTGTAACAGACGCAATAAGCAACCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCAt                                                                                                                             <  2:78105/151‑1 (MQ=255)                          aGTTCACTGGTTTGTAACAGACGCAATAAGCAACCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACt                                                                                                                       <  1:653102/151‑1 (MQ=255)                              cACTGGATTGTAACAGACGCAATAAGCAACCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGGGGCTCAACAGGAAGCGTTTCGCCATTCCACTg                                                                                                                      >  2:63791/1‑148 (MQ=255)                               aCTGGTTTGTAACAGACGCAATAAGCAACCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGcgcg                                                                                                                  <  2:269346/151‑1 (MQ=255)                                  ggTTTGTAACAGACGCAATAAGCAACCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCga                                                                                                                 <  1:805485/149‑1 (MQ=255)                                    tttGTAACAGACGCAATAAGCAACCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGc                                                                                                             >  2:648372/1‑151 (MQ=255)                                       gTAACAGACGCAATAAGCAACCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCtt                                                                                                           >  1:157666/1‑150 (MQ=255)                                                gCAATAAGCAACCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATcgcg                                                                                                 >  2:12988/1‑151 (MQ=255)                                                    tAAGCAACCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACg                                                                                              >  1:52693/1‑150 (MQ=255)                                                      aGCAACCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTaa                                                                                           <  1:825129/151‑1 (MQ=255)                                                        cAACCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAAc                                                                                         <  2:690753/151‑1 (MQ=255)                                                         aaCCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACg                                                                                        <  1:829821/151‑1 (MQ=255)                                                           ccTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGt                                                                                      <  2:742953/151‑1 (MQ=255)                                                                  acgTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTc                                                                               >  2:352227/1‑151 (MQ=255)                                                                   cgNTAATAGNNNNCNNTGNNNGNAAGCAAAACCGGCCAGTTCAANCCAGTCGTNGTNAGAAAGGACGG‑CATCGTTTAGTGGCTCANCANGAAGCNTTTCGCCATNCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATNc                                                                                 <  1:342090/148‑1 (MQ=255)                                                                      tAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACg                                                                            <  1:391282/150‑1 (MQ=255)                                                                         tAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTc                                                                         >  2:816072/1‑150 (MQ=255)                                                                          aGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTca                                                                        <  2:127811/150‑1 (MQ=255)                                                                           gCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTcagc                                                                      <  1:921617/151‑1 (MQ=255)                                                                           gCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTcagc                                                                      >  1:909438/1‑151 (MQ=255)                                                                           gCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTcagc                                                                      <  1:841308/151‑1 (MQ=255)                                                                             ggACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTcagcca                                                                    <  2:811121/151‑1 (MQ=255)                                                                               aCGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTcagccagc                                                                  <  1:198400/151‑1 (MQ=255)                                                                                cGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCt                                                                 >  2:658351/1‑151 (MQ=255)                                                                                  aTGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGt                                                               >  2:109156/1‑151 (MQ=255)                                                                                   tGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGtt                                                              >  2:806262/1‑151 (MQ=255)                                                                                      gCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTa                                                            <  2:836434/150‑1 (MQ=255)                                                                                         aaaGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCg                                                        <  2:111889/151‑1 (MQ=255)                                                                                         aaaGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCg                                                        <  2:200674/151‑1 (MQ=255)                                                                                            gCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCAt                                                     <  1:56015/151‑1 (MQ=255)                                                                                              aaaaCCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATc                                                    >  2:94266/1‑150 (MQ=255)                                                                                                 aCCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTc                                                <  2:562261/151‑1 (MQ=255)                                                                                                     gCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCg                                            <  1:504445/151‑1 (MQ=255)                                                                                                        aGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGt                                          >  2:674350/1‑150 (MQ=255)                                                                                                            cAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAAc                                       >  2:521355/1‑149 (MQ=255)                                                                                                             aaGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAgc                                    >  1:164983/1‑151 (MQ=255)                                                                                                             aaGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAgc                                    <  2:32879/151‑1 (MQ=255)                                                                                                              aGCCCGTCGGCGTTCGAAAGGACGGCCACCGTTAAGGGCCTC‑ACCGGAAACGTTTCCGCCTTTCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACa                                      <  1:670731/148‑1 (MQ=255)                                                                                                                     cgtcgtcAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGc                            <  1:23596/151‑1 (MQ=255)                                                                                                                      atcgtcAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGcc                           >  1:66317/2‑151 (MQ=255)                                                                                                                           cAGAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGtat                       <  1:718763/150‑1 (MQ=255)                                                                                                                             gAAAGGACGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAg                     >  1:297111/1‑150 (MQ=255)                                                                                                                                   aCGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCgc              <  1:767407/151‑1 (MQ=255)                                                                                                                                    cGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCgct             <  1:652206/151‑1 (MQ=255)                                                                                                                                    cGG‑CATCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCgct             <  1:526329/151‑1 (MQ=255)                                                                                                                                         aTCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGctgctg         >  2:795471/1‑151 (MQ=255)                                                                                                                                          tCGTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTgg        >  2:809917/1‑151 (MQ=255)                                                                                                                                            gTTTAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCt      >  1:208078/1‑151 (MQ=255)                                                                                                                                               tAGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTcc   >  1:323034/1‑151 (MQ=255)                                                                                                                                                aGTGGCTCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTccc  <  2:897488/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  ACGCCCACGCAGCGCCGGTAATGCCAGTTCACTGGTTTGTAACAGACGCAATAAGCAACCTAACGACGTTAATAGCGGACGATGAGCGAAAGCAAAACCGGCCAGTTCAAGCCAGTCGTCGTCAGAAAGGACGG‑CATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCC  >  NC_000913/2594015‑2594307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑