Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,626,437	T→G	L441L (CTT→CTG)	ppx →	exopolyphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,626,437	0	T	G	100.0%	188.1 / NA	52	L441L (CTT→CTG)	ppx	exopolyphosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (24/28);  total (24/28)

ACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTTGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCGTTTCCCGCATGACTGGTTTAGTCAGAATGCGCTGGTACTGCTTGATCTGGAAAAGGAGCA  >  NC_000913/2626292‑2626583                                                                                                                                                  |                                                                                                                                                   aCTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGt                                                                                                                                               <  1:13932/151‑1 (MQ=255)  cTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGt                                                                                                                                               >  1:283488/1‑150 (MQ=255)    tGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTAtt                                                                                                                                            >  1:496670/1‑151 (MQ=255)      ccGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTAc                                                                                                                                          >  2:322185/1‑151 (MQ=255)          gTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTca                                                                                                                                       >  1:171711/1‑150 (MQ=255)           ttttAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTcaac                                                                                                                                     >  2:526905/1‑151 (MQ=255)             ttAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCCCCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTcaaca                                                                                                                                    <  1:839984/150‑1 (MQ=255)             ttAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTcaaca                                                                                                                                    <  2:245584/150‑1 (MQ=255)                      aaCAGCAGCTGATGATGGCGACACTGGTGCGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTc                                                                                                                           >  2:743810/1‑150 (MQ=255)                              cTGATGATGGCGACACTGGTGCGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAacc                                                                                                                   >  1:919711/1‑150 (MQ=255)                                   gatgGCGACACTGGTGCGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACcaca                                                                                                             <  2:54610/151‑1 (MQ=255)                                    atgGCGACACTGGTGCGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCacac                                                                                                            <  2:184554/151‑1 (MQ=255)                                     gggCGCCACTGGCGCGCTCTCACCGTAAAGCGATTAAGCCCGTCGATTTACCGCGCTTTACCTTGTTTAAAAAGAAACTGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACAcc                                                                                                           <  1:863739/150‑1 (MQ=255)                                               ggTGCGCTATCACCGTAAAGCGATTAAGCTCGCCGATCTCCCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTg                                                                                                 <  2:902552/151‑1 (MQ=255)                                               ggTGCGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACATTCCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACAtt                                                                                                  <  1:735151/150‑1 (MQ=255)                                                        tCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTCCCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTg                                                                                           <  1:493975/148‑1 (MQ=255)                                                               aaaGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACtgatg                                                                                 <  2:765419/151‑1 (MQ=255)                                                                aaGCGATTAAGCTCGACGATCTCCCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACtgatga                                                                                <  1:839118/151‑1 (MQ=255)                                                                aaGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACtgatga                                                                                <  2:194782/151‑1 (MQ=255)                                                                    gATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAg                                                                             <  2:513589/150‑1 (MQ=255)                                                                              gacgaTCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGacac                                                                  >  2:627147/1‑151 (MQ=255)                                                                               acgaTCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGacac                                                                  <  2:654871/150‑1 (MQ=255)                                                                               acgaTCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACt                                                                 >  2:613752/1‑151 (MQ=255)                                                                                cgaTCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACt                                                                 <  2:814483/150‑1 (MQ=255)                                                                                   tCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCGt                                                             >  1:426933/1‑151 (MQ=255)                                                                                   tCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCGt                                                             >  2:835993/1‑151 (MQ=255)                                                                                       ccGCGCTTTCCCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCg                                                              <  1:456791/146‑1 (MQ=255)                                                                                       ccGCGCTTTCCCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCGt                                                             <  1:936558/147‑1 (MQ=255)                                                                                        cgcgCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCGTTTccc                                                        >  1:137907/1‑151 (MQ=255)                                                                                        cgcgCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCGTTTccc                                                        <  1:298333/151‑1 (MQ=255)                                                                                        cgcgCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCGTTTccc                                                        >  1:732375/1‑151 (MQ=255)                                                                                          cgcTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCGTTTCCCg                                                       <  2:320998/150‑1 (MQ=255)                                                                                              ttACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCGTTTCCCGCATg                                                   <  2:56272/150‑1 (MQ=255)                                                                                                     gTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCGTTTCCCGCATGACTGGttt                                           >  2:717361/1‑151 (MQ=255)                                                                                                       ttAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCGTTTCCCGCATGACTGGTTTa                                          <  2:3899/150‑1 (MQ=255)                                                                                                        tAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCGTTTCCCGCATGACTGGTTTAGt                                        >  1:654045/1‑151 (MQ=255)                                                                                                              gaaACAGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCGTTTCCCGCATGACTGGTTTAGTCAGAAt                                  >  2:270280/1‑151 (MQ=255)                                                                                                                aaCAGTCCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCGTTTCCCGCATGACTGGTTTAGTCAGAATgc                                <  1:1092/151‑1 (MQ=255)                                                                                                                   aGTTCCTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCGTTTCCCGCATGACTGGTTTAGTCAGAATGCGCt                             >  2:915462/1‑151 (MQ=255)                                                                                                                       ccTGCCACTGATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCGTTTCCCGCATGACTGGTTTAGTCAGAATGCGCTgg                           <  1:847732/149‑1 (MQ=255)                                                                                                                                gATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCGTTTCCCGCATGACTGGTTTAGTCAGAATGCGCTGGTACTGCTTg                  <  2:711125/149‑1 (MQ=255)                                                                                                                                gATACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCGTTTCCCGCATGACTGGTTTAGTCAGAATGCGCTGGTACTGCTTGAt                >  2:708555/1‑151 (MQ=255)                                                                                                                                 aTACAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACATTCACTGGACACTGCGTTTCCCGCATGACTGGTTTAGTCAGAATGCGCTGGTACTGCTTGATc               >  1:221227/1‑151 (MQ=255)                                                                                                                                    cAGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCGTTTCCCGCATGACTGGTTTAGTCAGAATGCGCTGGTACTGCTTGATCTg             <  1:742987/150‑1 (MQ=255)                                                                                                                                     aGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCGTTTCCCGCATGACTGGTTTAGTCAGAATGCGCTGGTACTGCTTGATCTgg            >  1:322262/1‑150 (MQ=255)                                                                                                                                     aGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCGTTTCCCGCATGACTGGTTTAGTCAGAATGCGCTGGTACTGCTTGATCTGGa           <  1:508043/151‑1 (MQ=255)                                                                                                                                     aGCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCGTTTCCCGCATGACTGGTTTAGTCAGAATGCGCTGGTACTGCTTGATCTGGa           <  2:875916/151‑1 (MQ=255)                                                                                                                                      gCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCGTTTCCCGCATGACTGGTTTAGTCAGAATGCGCTGGTACTGCTTGATCTGGaa          <  2:701153/151‑1 (MQ=255)                                                                                                                                      gCTATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCGTTTCCCGCATGACTGGTTTAGTCAGAATGCGCTGGTACTGCTTGATCTGGa           >  1:249783/1‑150 (MQ=255)                                                                                                                                        tATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCGTTTCCCGCATGACTGGTTTAGTCAGAATGCGCTGGTACTGCTTGATCTGGaaa         >  2:786492/1‑150 (MQ=255)                                                                                                                                        tATTGCGCCTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCGTTTCCCGCATGACTGGTTTAGTCAGAATGCGCTGGTACTGCTTGATCTGGaaa         >  2:119271/1‑150 (MQ=255)                                                                                                                                               ccTGGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCGTTTCCCGCATGACTGGTTTAGTCAGAATGCGCTGGTACTGCTTGATCTGGAAAAGGAGCa  >  1:483039/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                   ACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTTGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACACCGCCAACATTGACGCTGATTACTGATGACAGTCACTGGACACTGCGTTTCCCGCATGACTGGTTTAGTCAGAATGCGCTGGTACTGCTTGATCTGGAAAAGGAGCA  >  NC_000913/2626292‑2626583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑