Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,683,710	A→C	Q283P (CAA→CCA)	yphH →	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,683,710	0	A	C	100.0%	187.0 / NA	50	Q283P (CAA→CCA)	yphH	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (27/23);  total (27/23)

GTAGCGGACAGATTGGTCACACCATCGTTAATCCGGATGGCGTCGTCTGCGACTGTGGACGTTATGGCTGCCTGGAAACTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCAACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTCAATATT  >  NC_000913/2683563‑2683849                                                                                                                                                    |                                                                                                                                            gTAGCGGACAGATTGGTCACACCATCGTTAATCCGGATGGCGTCGTCTGCGACTGTGGACGTTATGGCTGCCTGGAAACTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACt                                                                                                                                          >  1:873332/1‑151 (MQ=255)      ggACAGATTGGTCACACCATCGTTAATCCGGATGGCGTCGTCTGCGACTGTGGACGTTATGGCTGCCTGGAAACTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATc                                                                                                                                     >  1:893872/1‑151 (MQ=255)      ggACAGATTGGTCACACCATCGTTAATCCGGATGGCGTCGTCTGCGACTGTGGACGTTATGGCTGCCTGGAAACTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATc                                                                                                                                     >  1:819087/1‑151 (MQ=255)      ggACAGATTGGTCACACCATCGTTAATCCGGATGGCGTCGTCTGCGACTGTGGACGTTATGGCTGCCTGGAAACTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATc                                                                                                                                     >  1:517781/1‑151 (MQ=255)         cAGATTGGTCACACCATCGTTAATCCGGATGGCGTCGTCTGCGACTGTGGACGTTATGGCTGCCTGGAAACTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTg                                                                                                                                  <  2:431629/151‑1 (MQ=255)                          cGTTAATCCGGATGGCGTCGTCTGCGACTGTGGACGTTATGGCTGCCTGGAAACTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAgc                                                                                                                  <  2:11048/150‑1 (MQ=255)                             tAATCCGGATGGCGTCGTCTGCGACTGTGGACGTTATGGCTGCCTGGAAACTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCa                                                                                                               <  2:543643/150‑1 (MQ=255)                              aaTCCGGATGGCGTCGTCTGCGACTGTGGACGTTATGGCTGCCTGGAAACTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGt                                                                                                             >  1:173969/1‑151 (MQ=255)                               aTCCGGATGGCGTCGTCTGCGACTGTGGACGTTATGGCTGCCTGGAAACTATCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGtt                                                                                                            <  2:155255/151‑1 (MQ=255)                                 ccGGATGGCGTCGTCTGCGACTGTGGACGTTATGGCTGCCTGGAAACTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTa                                                                                                           <  1:314537/150‑1 (MQ=255)                                    gATGGCGTCGTCTGCGACTGTGGACGTTATGGCTGCCTGGAAACTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCg                                                                                                       <  1:380103/151‑1 (MQ=255)                                            cgtcTGCGACTGTGGACGTTATGGCTGCCTGGAAACTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTgg                                                                                               >  1:688190/1‑151 (MQ=255)                                            cgtcTGCGACTGTGGACGTTATGGCTGCCTGGAAACTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGGATGGCTAAAATCACAACCGGTTAGATATCCACCTGACACTGAAAAACTGACTACAGCGCAGTTAATAGGTGCCttg                                                                                               >  1:97710/1‑149 (MQ=255)                                             gtcTGCGACTGTGGACGTTATGGCTGCCTGGAAACTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGc                                                                                              >  2:85122/1‑151 (MQ=255)                                                 gCGACTGTGGACGTTATGGCTGCCTGGAAACTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCaa                                                                                            <  2:434298/149‑1 (MQ=255)                                                    aCTGTGGACGTTATGGCTGCCTGGAAACTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTgg                                                                                       >  2:875859/1‑151 (MQ=255)                                                                  ggCTGCCTGGAAACTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATc                                                                          <  2:628053/150‑1 (MQ=255)                                                                  ggCTGCCTGGAAACTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATc                                                                          <  1:835838/150‑1 (MQ=255)                                                                      gCCTGGAAACTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCAcc                                                                       >  1:273948/1‑149 (MQ=255)                                                                      gCCTGGAAACTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAg                                                                     <  2:271330/151‑1 (MQ=255)                                                                      gCCTGGAAACTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAg                                                                     <  1:317335/151‑1 (MQ=255)                                                                       ccTGGAAACTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGc                                                                    >  1:832750/1‑151 (MQ=255)                                                                         tGGAAACTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTg                                                                  >  2:279316/1‑151 (MQ=255)                                                                           gAAACTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGAATACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTggg                                                                >  1:810100/1‑151 (MQ=255)                                                                           gAAACTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGGATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTggg                                                                >  1:832230/1‑151 (MQ=255)                                                                            aaaaTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAACTCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGAACACCAGCTGGGt                                                               >  1:448543/1‑151 (MQ=255)                                                                            aaaCTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTTGAGAACCGTGGATCACCAGCTGGGt                                                               <  1:185201/151‑1 (MQ=255)                                                                             aaCTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGtt                                                              >  2:680843/1‑151 (MQ=255)                                                                              aCTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTg                                                             >  2:46499/1‑151 (MQ=255)                                                                                 gTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATc                                                          <  2:819107/151‑1 (MQ=255)                                                                                     ccTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGctc                                                      >  2:64683/1‑151 (MQ=255)                                                                                         gTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGcc                                                  <  2:819096/151‑1 (MQ=255)                                                                                              gcgcATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGc                                             <  1:641110/151‑1 (MQ=255)                                                                                              gcgcATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGc                                             <  2:807857/151‑1 (MQ=255)                                                                                                       aaaaaCAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGttt                                    <  1:214210/151‑1 (MQ=255)                                                                                                          aaCAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTg                                   >  2:165288/1‑149 (MQ=255)                                                                                                          aaCAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTg                                   >  2:78399/1‑149 (MQ=255)                                                                                                            cAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTc                               <  1:465063/151‑1 (MQ=255)                                                                                                             aGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCt                              <  2:45506/151‑1 (MQ=255)                                                                                                             aGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCt                              <  2:7461/151‑1 (MQ=255)                                                                                                                cgcgGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGt                            >  1:417609/1‑150 (MQ=255)                                                                                                                  cgGGTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGtat                          >  1:673884/1‑150 (MQ=255)                                                                                                                   gggTATGGCTAAAATCACAACCGGTTAGTACTCCACTTGGTCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGTATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGtata                         <  1:731136/150‑1 (MQ=255)                                                                                                                        tGGCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTcc                   >  2:925390/1‑151 (MQ=255)                                                                                                                          gCTAAAATCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTc                 <  1:548138/151‑1 (MQ=255)                                                                                                                             aaaaTCACAACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTCAAc              >  1:6136/1‑151 (MQ=255)                                                                                                                                  cacaACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCt         >  2:806222/1‑151 (MQ=255)                                                                                                                                  cacaACCGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCt         >  2:401661/1‑151 (MQ=255)                                                                                                                                       ccGGTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTCAata    <  2:917898/151‑1 (MQ=255)                                                                                                                                         ggTTAGTACTCCACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTCAATAtt  >  2:447276/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                            GTAGCGGACAGATTGGTCACACCATCGTTAATCCGGATGGCGTCGTCTGCGACTGTGGACGTTATGGCTGCCTGGAAACTGTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTTAGTACTCAACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTCAATATT  >  NC_000913/2683563‑2683849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑