Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,690,978	T→G	S41R (AGC→CGC)	glrK ←	sensor protein kinase regulating glmY sRNA in two‑component system with response regulator GlrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,690,978	0	T	G	100.0%	253.9 / NA	70	S41R (AGC→CGC)	glrK	sensor protein kinase regulating glmY sRNA in two‑component system with response regulator GlrR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (38/32);  total (38/32)

GGCTTTGATAAACCTTCGCCAGGGTTGGGTCGTCCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAAT  >  NC_000913/2690831‑2691123                                                                                                                                                    |                                                                                                                                                  ggCTTTGATAAACCTTCGCCAGGGTTGGGTCGTCCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGaa                                                                                                                                                 <  1:550348/150‑1 (MQ=255)     ttGATAAACCTTCGCCAGGGTTGGGTCGTCCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAgcgc                                                                                                                                             >  1:450233/1‑150 (MQ=255)     ttGATAAACCTTCGCCAGGGTTGGGTCGTCCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAgcg                                                                                                                                              <  2:163273/149‑1 (MQ=255)            aCCTTCGCCAGGGTTGGGTCGTCCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGc                                                                                                                                     >  2:262206/1‑151 (MQ=255)             ccTTCGCCAGGGTTGGGTCGTCCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCt                                                                                                                                    <  1:315564/151‑1 (MQ=255)                tCGCCAGGGTTGGGTCGTCCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCtt                                                                                                                                   <  1:678384/149‑1 (MQ=255)                 cGCCAGGGTTGGGTCGTCCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGc                                                                                                                                >  1:808407/1‑151 (MQ=255)                  gCCAGGGTTGGGTCGTCCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTg                                                                                                                                 <  1:263285/149‑1 (MQ=255)                  gCCAGGGTTGGGTCGTCCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGcc                                                                                                                               <  2:809744/151‑1 (MQ=255)                   ccAGGGTTGGGTCGTCCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGcc                                                                                                                               >  1:186028/1‑150 (MQ=255)                   ccAGGGTTGGGTCGTCCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGcc                                                                                                                               >  1:454647/1‑150 (MQ=255)                      gggTTGGGTCGTCCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGc                                                                                                                           >  2:284348/1‑151 (MQ=255)                           gggTCGTCCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTtgcc                                                                                                                      >  2:273434/1‑151 (MQ=255)                           gggTCGTCCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTtgcc                                                                                                                      >  2:255897/1‑151 (MQ=255)                            ggTCGTCCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTtgcca                                                                                                                     <  1:841640/151‑1 (MQ=255)                                       aCGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCa                                                                                                          <  2:45939/151‑1 (MQ=255)                                            aTACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAgg                                                                                                     <  2:453828/151‑1 (MQ=255)                                             tACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAggg                                                                                                    >  1:164814/1‑151 (MQ=255)                                             tACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAggg                                                                                                    >  2:684032/1‑151 (MQ=255)                                                tGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGgc                                                                                                  >  1:484394/1‑150 (MQ=255)                                                tGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGgc                                                                                                  >  1:47026/1‑150 (MQ=255)                                                      tAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGa                                                                                            >  2:79687/1‑150 (MQ=255)                                                               tCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCaaa                                                                                   >  2:30149/1‑150 (MQ=255)                                                                ccATCGCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCaaaa                                                                                  <  1:812291/150‑1 (MQ=255)                                                                 cATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAAt                                                                                <  2:527980/151‑1 (MQ=255)                                                                     tcCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGcc                                                                             >  2:453408/1‑150 (MQ=255)                                                                       cAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGc                                                                          >  1:29797/1‑151 (MQ=255)                                                                          cgccgcGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCAtt                                                                       <  2:934634/151‑1 (MQ=255)                                                                              gcgTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTAcc                                                                    >  2:430325/1‑150 (MQ=255)                                                                              gcgTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTAcc                                                                    >  1:315220/1‑150 (MQ=255)                                                                                  tGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGtt                                                                <  2:332209/150‑1 (MQ=255)                                                                                   ggTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGt                                                              >  2:219200/1‑151 (MQ=255)                                                                                       aTCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTa                                                          <  2:689092/151‑1 (MQ=255)                                                                                       aTCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTa                                                          <  2:61529/151‑1 (MQ=255)                                                                                         cGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAAt                                                        >  2:587752/1‑151 (MQ=255)                                                                                          gCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATg                                                       >  2:811350/1‑151 (MQ=255)                                                                                          gCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATg                                                       <  2:78924/151‑1 (MQ=255)                                                                                          gCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATg                                                       >  2:607630/1‑151 (MQ=255)                                                                                             tCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGc                                                    >  1:438892/1‑151 (MQ=255)                                                                                               aCTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCgg                                                  <  2:454651/151‑1 (MQ=255)                                                                                                  gcgcCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCgggg                                                <  2:193544/150‑1 (MQ=255)                                                                                                  gcgcCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGAAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCgggg                                                <  2:188544/150‑1 (MQ=255)                                                                                                         gCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACgg                                        <  1:511019/151‑1 (MQ=255)                                                                                                          cATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACggg                                       >  1:817800/1‑151 (MQ=255)                                                                                                          cATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACggg                                       >  2:64365/1‑151 (MQ=255)                                                                                                          cATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACggg                                       >  2:115670/1‑151 (MQ=255)                                                                                                             cAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGcc                                     <  1:284340/150‑1 (MQ=255)                                                                                                             cAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGcc                                     <  1:189307/150‑1 (MQ=255)                                                                                                             cAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGcc                                     <  1:397271/150‑1 (MQ=255)                                                                                                             cAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCa                                    <  1:161269/151‑1 (MQ=255)                                                                                                                tAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCg                                 >  1:99581/1‑151 (MQ=255)                                                                                                                 aaGCGTAGTGCGGTTATCCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCg                                 <  1:280603/150‑1 (MQ=255)                                                                                                                 aaGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGt                                >  2:426862/1‑151 (MQ=255)                                                                                                                         tGCGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGgt                        >  2:379978/1‑151 (MQ=255)                                                                                                                           cGGTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGgtgt                      >  1:403049/1‑151 (MQ=255)                                                                                                                            ggTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGtt                     <  2:132346/151‑1 (MQ=255)                                                                                                                             gTTAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTa                    <  1:651326/151‑1 (MQ=255)                                                                                                                              ttAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTa                    <  1:408602/150‑1 (MQ=255)                                                                                                                              ttAACCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTAc                   >  1:219174/1‑151 (MQ=255)                                                                                                                                aaCCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTActc                 <  2:483159/151‑1 (MQ=255)                                                                                                                                 aCCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTActct                >  1:523707/1‑151 (MQ=255)                                                                                                                                 aCCAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTActct                >  2:240904/1‑151 (MQ=255)                                                                                                                                   cAGCGCCGCCTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCg              >  2:908316/1‑151 (MQ=255)                                                                                                                                       gccgccTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTc            >  2:650022/1‑149 (MQ=255)                                                                                                                                       gccgccTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTc            >  2:652912/1‑149 (MQ=255)                                                                                                                                          gccTGATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGAcg       <  2:667662/151‑1 (MQ=255)                                                                                                                                              gATCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGAcgcg     <  1:577756/149‑1 (MQ=255)                                                                                                                                               aTCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAAt  >  2:649262/1‑151 (MQ=255)                                                                                                                                               aTCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAAt  <  2:301392/151‑1 (MQ=255)                                                                                                                                                tCGCGAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAAt  >  1:609618/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                  GGCTTTGATAAACCTTCGCCAGGGTTGGGTCGTCCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAAT  >  NC_000913/2690831‑2691123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑