Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,731,287	A→C	intergenic (‑130/+313)	rrsG ← / ← clpB	16S ribosomal RNA of rrnG operon/protein disaggregation chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,731,287	0	A	C	100.0%	74.1 / NA	23	intergenic (‑130/+313)	rrsG/clpB	16S ribosomal RNA of rrnG operon/protein disaggregation chaperone
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (15/8);  total (15/8)

GATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTGAGACTTGGTATTCATTTTTCGTCTTGCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGC  >  NC_000913/2731166‑2731431                                                                                                                          |                                                                                                                                                 gATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTGAGACTTGGTATTCATTTTTCGTCTTGCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATACATTGTTAAAGAGCAGTGCCGCTTCGCttt                                                                                                                     >  2:620736/1‑151 (MQ=14)  aTGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTGAGACTTGGTATTCATTTTTCGTCTTGCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATACATTGTTAAAGAGCAGTGCCGCTTCGCtttt                                                                                                                    >  1:732620/1‑151 (MQ=14)  aTGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTGAGACTTGGTATTCATTTTTCGTCTTGCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATACATTGTTAAAGAGCAGTGCCGCTTCGCtttt                                                                                                                    <  2:35429/151‑1 (MQ=14)                                                             aTTTTTCGTCTTGCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATACATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCttt                                                         >  2:515945/1‑151 (MQ=14)                                                                        tGCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATACATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTctct                                               <  1:308875/150‑1 (MQ=255)                                                                         gCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATACATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGt                                             <  2:285996/151‑1 (MQ=255)                                                                          cGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATACATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGtt                                            >  2:287797/1‑151 (MQ=255)                                                                           gACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATACATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg                                           >  2:701817/1‑151 (MQ=255)                                                                              gTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATACATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg                                           >  2:717645/1‑148 (MQ=255)                                                                                tAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATACATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGAtt                                      >  2:610110/1‑151 (MQ=255)                                                                                        cGTATCTTCGAGTGCCCACACAGATTGTCTGATACATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAgg                                >  1:560063/1‑149 (MQ=255)                                                                                             cTTCGAGTGCCCACACAGATTGTCTGATACATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAccc                         >  2:305159/1‑151 (MQ=255)                                                                                               tCGAGTGCCCACACAGATTGTCTGATACATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAcc                          <  1:731586/148‑1 (MQ=255)                                                                                                cGAGTGCCCACACAGATTGTCTGATACATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCccgc                      <  1:296694/151‑1 (MQ=255)                                                                                                 gAGTGCCCACACAGATTGTCTGATACATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCccgcc                     >  2:151095/1‑151 (MQ=255)                                                                                                     gCCCACACAGATTGTCTGATACATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGAcc                 >  2:244190/1‑151 (MQ=255)                                                                                                      cccACACAGATTGTCTGATACATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTCCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTAAGGAGAACCCCGCCGAc                  <  1:435709/149‑1 (MQ=255)                                                                                                       ccACACAGATTGTCTGATACATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCcg               <  1:603055/151‑1 (MQ=255)                                                                                                         acacaGATTGTCTGATACATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCcggc             >  1:388859/1‑151 (MQ=255)                                                                                                         acacaGATTGTCTGATACATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCcggc             >  2:710204/1‑151 (MQ=255)                                                                                                            caGATTGTCTGATACATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCggcggc          <  2:638737/151‑1 (MQ=255)                                                                                                               aTTGTCTGATACATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCgtg       >  1:928717/1‑151 (MQ=255)                                                                                                                    cTGATACATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGc  >  1:26804/1‑151 (MQ=255)                                                                                                                          |                                                                                                                                                 GATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTGAGACTTGGTATTCATTTTTCGTCTTGCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGC  >  NC_000913/2731166‑2731431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑