Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,814,754	T→G	intergenic (‑21/+129)	luxS ← / ← gshA	S‑ribosylhomocysteine lyase/glutamate‑cysteine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,814,754	0	T	G	100.0%	171.4 / NA	46	intergenic (‑21/+129)	luxS/gshA	S‑ribosylhomocysteine lyase/glutamate‑cysteine ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (23/23);  total (23/23)

GGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCC  >  NC_000913/2814606‑2814898                                                                                                                                                     |                                                                                                                                                 ggCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAAtttgtt                                                                                                                                                <  1:48251/151‑1 (MQ=255) ggCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAAtttgtt                                                                                                                                                <  1:752630/151‑1 (MQ=255) ggCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAAtttgtt                                                                                                                                                >  2:421759/1‑151 (MQ=255)  gCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAAtttgttt                                                                                                                                               <  1:822931/151‑1 (MQ=255)   cACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAAtttgttt                                                                                                                                               >  2:675800/1‑150 (MQ=255)          aaGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAGAAAttt                                                                                                                                       <  1:179957/151‑1 (MQ=255)               cAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTg                                                                                                                                   >  2:112137/1‑150 (MQ=255)                aGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGaa                                                                                                                                 <  2:217485/151‑1 (MQ=255)                  aTCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAAct                                                                                                                               <  2:294135/151‑1 (MQ=255)                      aaCACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACtcttt                                                                                                                           >  1:469385/1‑151 (MQ=255)                               aTTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAg                                                                                                                   >  2:229692/1‑150 (MQ=255)                                 tGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGc                                                                                                                 <  1:456466/150‑1 (MQ=255)                                 tGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGc                                                                                                                 <  1:155248/150‑1 (MQ=255)                                            gCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGta                                                                                                      <  2:912595/150‑1 (MQ=255)                                              ggggTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTatat                                                                                                   <  1:603080/151‑1 (MQ=255)                                              ggggTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTatat                                                                                                   >  2:303256/1‑151 (MQ=255)                                                   gtTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGaa                                                                                                >  1:475797/1‑149 (MQ=255)                                                       aTTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGAcgc                                                                                          >  1:196688/1‑151 (MQ=255)                                                          gTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACgcgc                                                                                        <  1:207168/150‑1 (MQ=255)                                                           ttttCGCCACCCGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCa                                                                                       <  1:292124/150‑1 (MQ=255)                                                            tttCGCCACCCGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCAt                                                                                      <  2:663538/150‑1 (MQ=255)                                                             ttCGCCACCCGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCAtt                                                                                     <  1:816549/150‑1 (MQ=255)                                                                gCCACCCGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGtt                                                                                 <  1:78857/151‑1 (MQ=255)                                                                      cGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTatcatc                                                                           >  2:614340/1‑151 (MQ=255)                                                                        aaCTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTatcatcat                                                                         >  2:259057/1‑151 (MQ=255)                                                                        aaCTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTatcatcat                                                                         <  1:88990/151‑1 (MQ=255)                                                                         aCTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTatcatcatc                                                                        >  1:151403/1‑151 (MQ=255)                                                                            gCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCt                                                                     >  1:152267/1‑151 (MQ=255)                                                                            gCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCt                                                                     >  1:642714/1‑151 (MQ=255)                                                                              aGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTg                                                                    >  1:252182/1‑150 (MQ=255)                                                                                 cgcTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAAtt                                                                <  2:351417/151‑1 (MQ=255)                                                                                 cgcTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAAt                                                                 >  2:476486/1‑150 (MQ=255)                                                                                      ccATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCag                                                             >  2:428085/1‑149 (MQ=255)                                                                                      ccATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAAGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCaga                                                            <  1:905668/150‑1 (MQ=255)                                                                                            gggTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGaa                                                      >  2:609118/1‑150 (MQ=255)                                                                                                aTGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAAtttt                                                 <  2:805530/151‑1 (MQ=255)                                                                                                  gATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTg                                                <  2:560851/150‑1 (MQ=255)                                                                                                          gtgAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCa                                        <  2:15787/150‑1 (MQ=255)                                                                                                           tgAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCAc                                       >  1:545690/1‑150 (MQ=255)                                                                                                           tgAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGCCGAGTCTGAGTATATGAAAGCCGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAGATTTTGGccacccac                                       >  2:658965/1‑150 (MQ=255)                                                                                                              aGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGt                                   <  1:879850/151‑1 (MQ=255)                                                                                                               gCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTg                                  <  2:674365/151‑1 (MQ=255)                                                                                                                    cTAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCtt                             >  1:54281/1‑151 (MQ=255)                                                                                                                     tAACAACGGCATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCttt                            >  1:896738/1‑151 (MQ=255)                                                                                                                              cATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTtttcttttct                   >  2:439040/1‑151 (MQ=255)                                                                                                                              cATTTAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTtttcttttct                   <  2:528946/151‑1 (MQ=255)                                                                                                                                 ttAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGt                 <  1:144422/150‑1 (MQ=255)                                                                                                                                 ttAGCCACCTCCGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTc                >  1:31706/1‑151 (MQ=255)                                                                                                                                           ccGGTAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGttt      <  1:866999/151‑1 (MQ=255)                                                                                                                                               tAATTTGTTTAAAAATTTTCTGAACTCTTTCTTTCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGTAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTcc  >  1:752641/1‑151 (MQ=255)                                                                                                                                               tAATTTGTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTcc  >  1:44873/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                 GGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCC  >  NC_000913/2814606‑2814898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑