Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,964,868	T→G	K96N (AAA→AAC)	thyA ←	thymidylate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,964,868	0	T	G	100.0%	204.2 / NA	54	K96N (AAA→AAC)	thyA	thymidylate synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (29/25);  total (29/25)

ACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTT  >  NC_000913/2964720‑2965014                                                                                                                                                     |                                                                                                                                                   aCGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGgtt                                                                                                                                                  >  2:586895/1‑151 (MQ=255)       ccAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCa                                                                                                                                              <  1:84637/149‑1 (MQ=255)        cAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATac                                                                                                                                           >  2:90546/1‑151 (MQ=255)           cgacATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATac                                                                                                                                           <  1:182906/145‑1 (MQ=255)            gcCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTg                                                                                                                                       <  1:888249/151‑1 (MQ=255)               aTTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGcc                                                                                                                                    >  2:172386/1‑151 (MQ=255)                  ttATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCg                                                                                                                                  >  1:349316/1‑150 (MQ=255)                      ccAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTc                                                                                                                             <  2:185145/151‑1 (MQ=255)                       cAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCGCTGGTTACCATACACTGGCCCGAGGTCg                                                                                                                            >  1:432245/1‑151 (MQ=255)                              ccTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGtttt                                                                                                                     <  1:239240/151‑1 (MQ=255)                              ccTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGtttt                                                                                                                     >  2:677011/1‑151 (MQ=255)                              ccTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGtttt                                                                                                                     >  1:456183/1‑151 (MQ=255)                                    ttCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCg                                                                                                                <  2:196614/150‑1 (MQ=255)                                     tCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCgg                                                                                                               <  1:641605/150‑1 (MQ=255)                                          gCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCAtt                                                                                                         >  2:872499/1‑151 (MQ=255)                                           cTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTc                                                                                                        >  1:570735/1‑151 (MQ=255)                                            tGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCg                                                                                                       >  2:727989/1‑151 (MQ=255)                                            tGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCg                                                                                                       >  1:691794/1‑151 (MQ=255)                                             gAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTc                                                                                                        <  1:267834/149‑1 (MQ=255)                                               aaCAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTcc                                                                                                    >  1:655117/1‑151 (MQ=255)                                                     aatGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGAt                                                                                               <  2:776212/150‑1 (MQ=255)                                                     aatGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGAt                                                                                               <  1:95385/150‑1 (MQ=255)                                                         cggcgCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTTGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGa                                                                                          <  1:533593/151‑1 (MQ=255)                                                         cggcgCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGa                                                                                          <  2:34871/151‑1 (MQ=255)                                                            cgcgAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACAt                                                                                       >  2:283871/1‑151 (MQ=255)                                                             gcgAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACAtt                                                                                      <  2:76098/151‑1 (MQ=255)                                                                   ccGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTc                                                                                >  2:21917/1‑151 (MQ=255)                                                                        tCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTAGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCgtg                                                                             >  1:111478/1‑149 (MQ=255)                                                                         cGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCgtgt                                                                            >  2:721042/1‑149 (MQ=255)                                                                         cGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTa                                                                           <  2:618769/150‑1 (MQ=255)                                                                          gTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGa                                                                         <  1:336374/151‑1 (MQ=255)                                                                            ttttCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGAt                                                                        <  2:78371/150‑1 (MQ=255)                                                                              ttCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATa                                                                       >  2:520335/1‑149 (MQ=255)                                                                                cAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCa                                                                   <  2:708481/151‑1 (MQ=255)                                                                                 aGCTGGCGCAGTACCGTAGTGTTATGGTCAACATGACGACCATCTGGCGTTGGCCAGGGGCGCCACTGGTTACCCTACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGCGACATTGTTTTCGTGTAGATCAg                                                                     >  1:700822/1‑148 (MQ=255)                                                                                     ggTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATg                                                                <  1:675483/149‑1 (MQ=255)                                                                                     ggTTCAGTACCGTAGTGATCAGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATg                                                                <  1:487140/149‑1 (MQ=255)                                                                                      gTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGtt                                                              >  2:287167/1‑150 (MQ=255)                                                                                      gTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGtt                                                              >  2:879885/1‑150 (MQ=255)                                                                                             aCCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGc                                                      >  1:153037/1‑151 (MQ=255)                                                                                             aCCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGc                                                      <  2:147545/151‑1 (MQ=255)                                                                                             aCCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGc                                                      >  1:660231/1‑151 (MQ=255)                                                                                              ccGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGCCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGc                                                      >  2:154909/1‑150 (MQ=255)                                                                                                      aTCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGa                                              >  1:887410/1‑150 (MQ=255)                                                                                                       tCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGaa                                             >  2:308327/1‑150 (MQ=255)                                                                                                          ggTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACca                                         <  1:581273/151‑1 (MQ=255)                                                                                                             cAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCGGAAACcaca                                       <  1:460108/150‑1 (MQ=255)                                                                                                                       acCATCTGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATgg                            >  1:641836/1‑151 (MQ=255)                                                                                                                             tGGCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGatgatg                      >  2:579169/1‑151 (MQ=255)                                                                                                                              ggCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATgg                     <  2:701479/151‑1 (MQ=255)                                                                                                                              ggCGTTGGCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATgg                     <  2:98035/151‑1 (MQ=255)                                                                                                                                     gCCAGGCGCGCCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGc                >  2:822650/1‑149 (MQ=255)                                                                                                                                             cgcCACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAAc      >  1:324337/1‑151 (MQ=255)                                                                                                                                                cACTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGtt   >  2:269676/1‑151 (MQ=255)                                                                                                                                                  cTGGTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGttt  <  1:82621/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   ACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTGCAGAAACCACAGCAGTTCATGGATGATGGAACGCAGGTGGCAACGTTT  >  NC_000913/2964720‑2965014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑