Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	184,410	A→C	V220V (GTT→GTG)	yaeI ←	phosphodiesterase with model substrate bis‑pNPP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	184,410	0	A	C	100.0%	205.3 / NA	61	V220V (GTT→GTG)	yaeI	phosphodiesterase with model substrate bis‑pNPP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (29/32);  total (29/32)

CAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCAACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGT  >  NC_000913/184263‑184560                                                                                                                                                    |                                                                                                                                                       cAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTcacccacc                                                                                                                                                     >  1:807444/1‑151 (MQ=255)   gTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCacccacca                                                                                                                                                    >  2:62780/1‑150 (MQ=255)     tCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACccaccaac                                                                                                                                                  <  2:115367/150‑1 (MQ=255)          cATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTAcg                                                                                                                                            <  1:788850/151‑1 (MQ=255)                  cTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTg                                                                                                                                    >  1:911967/1‑151 (MQ=255)                     ccGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGccc                                                                                                                                 >  2:853792/1‑151 (MQ=255)                         gcggcAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCccgccg                                                                                                                             <  2:753185/151‑1 (MQ=255)                             cAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTg                                                                                                                           >  2:689/1‑149 (MQ=255)                                ttCAGACGCAAACCATACAACCTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTg                                                                                                                           <  1:809564/146‑1 (MQ=255)                                ttCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTa                                                                                                                       <  2:600805/150‑1 (MQ=255)                                ttCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTAt                                                                                                                      >  1:147608/1‑151 (MQ=255)                                   aGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATgg                                                                                                                    <  1:497584/150‑1 (MQ=255)                                       gCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGca                                                                                                               <  2:569366/151‑1 (MQ=255)                                       gCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGc                                                                                                                >  1:453530/1‑150 (MQ=255)                                         aacccAAACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGcaca                                                                                                             <  1:584238/148‑1 (MQ=255)                                           aCCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGc                                                                                                           <  2:566319/151‑1 (MQ=255)                                            ccATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGc                                                                                                           >  1:329440/1‑150 (MQ=255)                                                aCAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCa                                                                                                       <  2:309585/150‑1 (MQ=255)                                                  aaaCTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGAt                                                                                                    <  2:812931/151‑1 (MQ=255)                                                   aaCTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCCCCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGAt                                                                                                    <  2:245590/150‑1 (MQ=255)                                                     cTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATcc                                                                                                  >  2:264974/1‑150 (MQ=255)                                                       gCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATccc                                                                                                 >  2:367837/1‑149 (MQ=255)                                                       gCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCa                                                                                                <  2:234952/150‑1 (MQ=255)                                                           acgccacgGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGtt                                                                                           >  2:60099/1‑151 (MQ=255)                                                            cgccacgGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTtc                                                                                          <  2:41147/151‑1 (MQ=255)                                                              ccacgGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTtcat                                                                                        >  1:600420/1‑151 (MQ=255)                                                              ccacgGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTtca                                                                                         <  1:756834/150‑1 (MQ=255)                                                                acgGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTtcatc                                                                                       >  2:786175/1‑150 (MQ=255)                                                                  gggTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCAc                                                                                     <  1:101400/150‑1 (MQ=255)                                                                      tgtgTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCAt                                                                                 <  1:321013/150‑1 (MQ=255)                                                                         gtAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGAct                                                                             <  1:407696/151‑1 (MQ=255)                                                                               gtgtCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCttt                                                                        <  1:730157/150‑1 (MQ=255)                                                                                tgtCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGc                                                                      >  1:511907/1‑151 (MQ=255)                                                                                tgtCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGc                                                                      >  1:729976/1‑151 (MQ=255)                                                                                   cTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGt                                                                   >  1:85274/1‑151 (MQ=255)                                                                                      tCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCg                                                                 <  1:852928/150‑1 (MQ=255)                                                                                      tCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCg                                                                 <  1:806751/150‑1 (MQ=255)                                                                                       cGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCggg                                                               <  1:583885/151‑1 (MQ=255)                                                                                         ccAAAGGCATTTAACCCGGCGACGTACCGTTTATCTTCGACGGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGAt                                                             <  1:503016/151‑1 (MQ=255)                                                                                         caaaaGGCATTTAATCCGGCGACGTAACGTTTATCTCCGACAGGGGCAAAAGGTTCACCCCCCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGAt                                                             <  1:541229/149‑1 (MQ=255)                                                                                          cAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGAtt                                                            >  2:501502/1‑151 (MQ=255)                                                                                          cAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGAtt                                                            >  1:86303/1‑151 (MQ=255)                                                                                            aaGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTAt                                                          <  2:353068/151‑1 (MQ=255)                                                                                              ggCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCCCCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATgc                                                        <  2:61426/151‑1 (MQ=255)                                                                                              ggCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATgc                                                        <  2:733133/151‑1 (MQ=255)                                                                                                 aTTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGcc                                                     >  1:432291/1‑151 (MQ=255)                                                                                                    tAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGc                                                  >  1:650107/1‑151 (MQ=255)                                                                                                      aTCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCAc                                                >  2:213836/1‑151 (MQ=255)                                                                                                         cGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAg                                             <  1:640566/151‑1 (MQ=255)                                                                                                           gCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTc                                           >  2:454418/1‑151 (MQ=255)                                                                                                            cGACGTAACGTTTATCTTCGACAGGGGCAAAAGTTTCACCCACCAACGGTACGCGCAGTTGCCCGGCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTc                                           >  1:875080/1‑150 (MQ=255)                                                                                                            cGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCt                                          <  2:269408/151‑1 (MQ=255)                                                                                                                     gTTTATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGAt                                  >  1:342264/1‑150 (MQ=255)                                                                                                                        tATCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGc                              >  1:479444/1‑151 (MQ=255)                                                                                                                          tCTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCtt                            >  2:90474/1‑151 (MQ=255)                                                                                                                           cTTCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTc                           >  2:547834/1‑151 (MQ=255)                                                                                                                            ttCGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCg                          >  2:120481/1‑151 (MQ=255)                                                                                                                              cGACAGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCt                        >  1:558549/1‑151 (MQ=255)                                                                                                                                  aGGGGCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCg                    >  2:266779/1‑151 (MQ=255)                                                                                                                                   ggggCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCgg                   <  1:30531/151‑1 (MQ=255)                                                                                                                                      gCAAAAGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAg                <  2:95414/151‑1 (MQ=255)                                                                                                                                         aaaGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCg             >  1:253237/1‑151 (MQ=255)                                                                                                                                          aaGGTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCgg            <  2:637480/151‑1 (MQ=255)                                                                                                                                            ggTTCACCCACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGtt          <  1:108782/151‑1 (MQ=255)                                                                                                                                                    aaccaacGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCAtttt  >  2:404263/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                       CAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAGGTTCACCAACCAACGGTACGCGCAGTTGCCCGCCGTGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGT  >  NC_000913/184263‑184560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑