Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,993,609	T→G	intergenic (+139/‑29)	ygeH → / → ygeI	putative transcriptional regulator/uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,993,609	0	T	G	100.0%	221.3 / NA	59	intergenic (+139/‑29)	ygeH/ygeI	putative transcriptional regulator/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (30/29);  total (30/29)

CGATAAATTTTTATTGACGCAATTTTATTATTTAGATGTATGCAACTTGTTATTTAAACAACAGAGATAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATTGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGAATGCAACGGGTGATGAAGTCGTATCATTAGATAAACACATCAACACCAGTGCTACTGATACAGA  >  NC_000913/2993465‑2993753                                                                                                                                                 |                                                                                                                                                 cGATAAATTTTTATTGACGCAATTTTATTATTTAGATGTATGCAACTTGTTATTTAAACAACAGAGATAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTg                                                                                                                                            <  2:121155/151‑1 (MQ=255)    tAAATTTTTATTGACGCAATTTTATTATTTAGATGTATGCAACTTGTTATTTAAACAACAGAGATAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGAc                                                                                                                                         >  2:222790/1‑151 (MQ=255)     aaaTTTTTATTGACGCAATTTTATTATTTAGATGTATGCAACTTGTTATTTAAACAACAGAGATAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGAc                                                                                                                                         <  2:372818/150‑1 (MQ=255)      aaTTTTTATTGACGCAATTTTATTATTTAGATGTATGCAACTTGTTATTTAAACAACAGAGATAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACa                                                                                                                                        >  2:458752/1‑150 (MQ=255)      aaTTTTTATTGACGCAATTTTATTATTTAGATGTATGCAACTTGTTATTTAAACAACAGAGATAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACa                                                                                                                                        >  1:116453/1‑150 (MQ=255)         ttttATTGACGCAATTTTATTATTTAGATGTATGCAACTTGTTATTTAAACAACAGAGATAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAAtt                                                                                                                                     >  2:203722/1‑150 (MQ=255)           ttATTGACGCAATTTTATTATTTAGATGTATGCAACTTGTTATTTAAACAACAGAGATAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTaa                                                                                                                                   <  2:456756/150‑1 (MQ=255)               tGACGCAATTTTATTATTTAGATGTATGCAACTTGTTATTTAAACAACAGAGATAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTaaaaa                                                                                                                                >  1:115798/1‑149 (MQ=255)                gACGCAATTTTATTATTTAGATGTATGCAACTTGTTATTTAAACAACAGAGATAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAg                                                                                                                              <  1:158720/150‑1 (MQ=255)                 aCGCAATTTTATTATTTAGATGTATGCAACTTGTTATTTAAACAACAGAGATAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAgg                                                                                                                             >  1:620258/1‑150 (MQ=255)                  cGCAATTTTATTATTTAGATGTATGCAACTTGTTATTTAAACAACAGAGATAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAggg                                                                                                                            >  1:382920/1‑150 (MQ=255)                    cAATTTTATTATTTAGATGTATGCAACTTGTTATTTAAACAACAGAGATAAAAATATCTAAGATATTCGCCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAg                                                                                                                          <  2:853363/150‑1 (MQ=255)                     aaTTTTATTATTTAGATGTATGCAACTTGTTATTTAAACAACAGAGATAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCt                                                                                                                        >  1:509770/1‑151 (MQ=255)                        tttATTATTTAGATGTATGCAACTTGTTATTTAAACAACAGAGATAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCttt                                                                                                                      >  1:333950/1‑150 (MQ=255)                          tattatTTAGATGTATGCAACTTGTTATTTAAACAACAGAGATAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATg                                                                                                                   >  1:590042/1‑151 (MQ=255)                             tattTAGATGTATGCAACTTGTTATTTAAACAACAGAGATAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACt                                                                                                                >  1:508518/1‑151 (MQ=255)                                  aGATGTATGCAACTTGTTATTTAAACAACAGAGATAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATcc                                                                                                           >  2:255206/1‑151 (MQ=255)                                  aGATGTATGCAACTTGTTATTTAAACAACAGAGATAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATc                                                                                                            <  2:147450/150‑1 (MQ=255)                                       tATGCAACTTGTTATTTAAACAACAGAGATAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCg                                                                                                      >  1:524572/1‑151 (MQ=255)                                          gCAACTTGTTATTTAAACAACAGAGATAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTa                                                                                                   <  1:356509/151‑1 (MQ=255)                                            aaCTTGTTATTTAAACAACAGAGATAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATc                                                                                                 >  1:876581/1‑151 (MQ=255)                                             aCTTGTTATTTAAACAACAGAGATAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATc                                                                                                 >  1:128063/1‑150 (MQ=255)                                                tGTTATTTAAACAACAGAGATAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCaat                                                                                              >  2:802609/1‑150 (MQ=255)                                                 gTTATTTAAACAACAGAGATAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCaataa                                                                                            <  1:494244/151‑1 (MQ=255)                                                   tATTTAAACAACAGAGATAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCaataat                                                                                           <  2:21320/150‑1 (MQ=255)                                                            aacaGAGATAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCaa                                                                                  >  1:548257/1‑150 (MQ=255)                                                                gagaTAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGtt                                                                              >  2:485944/1‑150 (MQ=255)                                                                   aTAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCaa                                                                           >  1:909626/1‑150 (MQ=255)                                                                       aaaTATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAAtata                                                                      <  2:268977/151‑1 (MQ=255)                                                                        aaTATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAg                                                                     <  2:642562/151‑1 (MQ=255)                                                                            tCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGa                                                                  >  1:911640/1‑150 (MQ=255)                                                                               aaGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGa                                                                  <  2:586460/147‑1 (MQ=255)                                                                               aaGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGAATGc                                                              >  2:865265/1‑151 (MQ=255)                                                                                aGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATAGAGCGAATGCa                                                             <  2:591070/151‑1 (MQ=255)                                                                                 gATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGAATGCa                                                             >  2:819773/1‑150 (MQ=255)                                                                                    atTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGAATGCAACgg                                                         >  1:520456/1‑151 (MQ=255)                                                                                      tCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGAATGCAACGGGt                                                       >  2:445973/1‑151 (MQ=255)                                                                                            tATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGAATGCAACGGGtgatga                                                  >  2:139302/1‑150 (MQ=255)                                                                                            tATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGAATGCAACGGGtgatga                                                  >  1:54102/1‑150 (MQ=255)                                                                                            tATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGAATGCAACGGGtgatg                                                   <  1:296529/149‑1 (MQ=255)                                                                                             aTTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGAATGCAACGGGTGATGAAg                                                <  2:394947/151‑1 (MQ=255)                                                                                               tGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGAATGCAACGGGTGATGAAGt                                               <  1:270115/150‑1 (MQ=255)                                                                                                gCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGAATGCAACGGGTGATGAAGTc                                              <  2:885630/150‑1 (MQ=255)                                                                                                  aaGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGAATGCAACGGGTGATGAAGTCGt                                            >  1:388792/1‑150 (MQ=255)                                                                                                    gATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGAATGCAACGGGTGATGAAGTCGTAta                                         <  2:892311/151‑2 (MQ=255)                                                                                                     atatTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGAATGCAACGGGTGATGAAGTCGTATCa                                        <  1:358875/151‑1 (MQ=255)                                                                                                       atTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGAATGCAACGGGTGATGAAGTCGTATCAt                                       <  1:212242/150‑1 (MQ=255)                                                                                                                   ctAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGAATGCAACGGGTGATGAAGTCGTATCATTAGATAAacac                            <  2:486128/149‑1 (MQ=255)                                                                                                                   ctAGAGTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGAATGCAACGGGTGATGAAGTCGTATCATTAGATAAacac                            <  1:107263/149‑1 (MQ=255)                                                                                                                     agagTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGAATGCAACGGGTGATGAAGTCGTATCATTAGATAAACACAt                          <  2:442369/149‑1 (MQ=255)                                                                                                                      gagTTAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGAATGCAACGGGTGATGAAGTCGTATCATTAGATAAACACATCa                        >  2:414987/1‑150 (MQ=255)                                                                                                                          tAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGAATGCAACGGGTGATGAAGTCGTATCATTAGATAAACACATCAacac                    <  2:272046/150‑1 (MQ=255)                                                                                                                          tAAAAGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGAATGCAACGGGTGATGAAGTCGTATCATTAGATAAACACATCAacac                    <  2:454920/150‑1 (MQ=255)                                                                                                                             aaGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGAATGCAACGGGTGATGAAGTCGTATCATTAGATAAACACATCAACACCAGt                <  1:17927/151‑1 (MQ=255)                                                                                                                              aGTATGATGTAAAGACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGAATGCAACGGGTGATGAAGTCGTATCATTAGATAAACACATCAACACCAGTg               <  1:227462/151‑1 (MQ=255)                                                                                                                                           gACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGAATGCAACGGGTGATGGAGTCGTATCATTAGATAAACACATCAACACCAGTGCTACTGATAc     >  2:850674/1‑148 (MQ=255)                                                                                                                                           gACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGAATGCAACGGGTGATGAAGTCGTATCATTAGATAAACACATCAACACCAGTGCTACTGATAc     >  2:544226/1‑148 (MQ=255)                                                                                                                                           gACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGAATGCAACGGGTGATGAAGTCGTATCATTAGATAAACACATCAACACCAGTGCTACTGATACAGa  <  1:841879/151‑1 (MQ=255)                                                                                                                                           gACCATGGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGAATGCAACGGGTGATGAAGTCGTATCATTAGATAAACACATCAACACCAGTGCTACTGATACAGa  <  1:257680/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                 CGATAAATTTTTATTGACGCAATTTTATTATTTAGATGTATGCAACTTGTTATTTAAACAACAGAGATAAAAATATCTAAGATATTCACCTTATTGCAAGATATTTAAAATGCTCTAGAGTTAAAAGTATGATGTAAAGACCATTGGCCTGGACAATTAAAAAAGGGAGCTTTATGACTAATCCAATCGGTATCAATAATTTATCTCAAAGTTCAAATATAGCGAATGCAACGGGTGATGAAGTCGTATCATTAGATAAACACATCAACACCAGTGCTACTGATACAGA  >  NC_000913/2993465‑2993753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑