Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,105,735	A→C	K8Q (AAA→CAA)	nupG →	nucleoside transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,105,735	0	A	C	100.0%	198.3 / NA	52	K8Q (AAA→CAA)	nupG	nucleoside transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (31/21);  total (31/21)

ATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCC  >  NC_000913/3105588‑3105877                                                                                                                                                    |                                                                                                                                               aTGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGcaa                                                                                                                                              >  1:205956/1‑150 (MQ=255) aTGTTTGCAAATAGTAGCCGCAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGcaaa                                                                                                                                             <  1:727199/151‑1 (MQ=255)   gTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATc                                                                                                                                           >  2:123826/1‑151 (MQ=255)          attattTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCtt                                                                                                                                    >  1:95040/1‑151 (MQ=255)          attattTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCtt                                                                                                                                    >  1:753561/1‑151 (MQ=255)           ttattTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCttt                                                                                                                                   >  2:834826/1‑151 (MQ=255)           ttattTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCttt                                                                                                                                   >  2:593293/1‑151 (MQ=255)            tattTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCtttt                                                                                                                                  >  2:929087/1‑151 (MQ=255)                  ccACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGc                                                                                                                              >  2:255452/1‑149 (MQ=255)                   cacaGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGt                                                                                                                           >  1:545815/1‑151 (MQ=255)                      aGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTtct                                                                                                                        <  1:562814/151‑1 (MQ=255)                        gTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTtctgt                                                                                                                      >  1:283179/1‑151 (MQ=255)                            cAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTctgtctgt                                                                                                                  <  1:734649/151‑1 (MQ=255)                             aaaaaaCCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCtgtg                                                                                                                 >  2:530625/1‑151 (MQ=255)                             aaaaaaCCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCtgtg                                                                                                                 >  1:639905/1‑151 (MQ=255)                              aaaaaCCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTgg                                                                                                                >  2:737639/1‑151 (MQ=255)                                 aaCCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTgggg                                                                                                              >  1:796833/1‑150 (MQ=255)                                      gTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTg                                                                                                        >  1:705467/1‑151 (MQ=255)                                       tCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTgg                                                                                                       >  1:226701/1‑151 (MQ=255)                                         cgcgAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCt                                                                                                     >  1:495857/1‑151 (MQ=255)                                           cgAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTga                                                                                                   <  1:634320/151‑1 (MQ=255)                                            gAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTgac                                                                                                  >  1:624403/1‑151 (MQ=255)                                                   aTAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTc                                                                                           <  1:521783/151‑1 (MQ=255)                                                     aGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCg                                                                                          <  1:884093/150‑1 (MQ=255)                                                       aaTCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGc                                                                                        >  2:673062/1‑150 (MQ=255)                                                       aaTCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCt                                                                                       <  1:421119/151‑1 (MQ=255)                                                         tCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTcc                                                                                     >  2:758438/1‑151 (MQ=255)                                                           ccATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCta                                                                                   <  1:184380/151‑1 (MQ=255)                                                             atcatcTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCtata                                                                                 <  2:689361/151‑1 (MQ=255)                                                             atcatcTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCtata                                                                                 <  2:235015/151‑1 (MQ=255)                                                                      cACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAtgtttgtt                                                                         >  1:471151/1‑150 (MQ=255)                                                                               aTGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGt                                                               <  2:120950/151‑1 (MQ=255)                                                                                  tgCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTg                                                            >  1:291046/1‑151 (MQ=255)                                                                                  tgCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTg                                                            >  2:654948/1‑151 (MQ=255)                                                                                  tgCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTg                                                            >  2:212312/1‑151 (MQ=255)                                                                                       tttCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGt                                                       >  1:276397/1‑151 (MQ=255)                                                                                            aaCACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTc                                                  <  1:6482/151‑1 (MQ=255)                                                                                             acacTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCt                                                 <  2:435285/151‑1 (MQ=255)                                                                                              cacTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTa                                                <  1:290985/151‑1 (MQ=255)                                                                                               acTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTAt                                               >  1:330612/1‑151 (MQ=255)                                                                                               acTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTAt                                               >  1:186451/1‑151 (MQ=255)                                                                                                        cATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGt                                      >  1:895407/1‑151 (MQ=255)                                                                                                           cACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGttt                                    <  1:736968/150‑1 (MQ=255)                                                                                                            aCGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTtat                                  <  2:554452/151‑1 (MQ=255)                                                                                                              gATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTtat                                  >  2:539085/1‑149 (MQ=255)                                                                                                                 gtgAGGAAATTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTc                             <  1:391024/151‑1 (MQ=255)                                                                                                                        aaTTAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGt                      <  2:911303/151‑1 (MQ=255)                                                                                                                           tAACATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTAt                    <  1:319542/150‑1 (MQ=255)                                                                                                                            aaCATGAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATc                   <  1:24142/150‑1 (MQ=255)                                                                                                                                 gAATCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCg             <  1:253637/151‑1 (MQ=255)                                                                                                                                  aaTCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCgg            >  2:858478/1‑151 (MQ=255)                                                                                                                                   aTCTTAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGt           >  1:415856/1‑151 (MQ=255)                                                                                                                                       tAAGCTGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCt         <  2:413294/149‑1 (MQ=255)                                                                                                                                            tGCAGCTGCAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGcc  >  1:621908/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                               ATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCC  >  NC_000913/3105588‑3105877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑