Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,107,858	A→C	L433R (CTG→CGG)	speC ←	ornithine decarboxylase, constitutive

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,107,858	0	A	C	100.0%	186.0 / NA	52	L433R (CTG→CGG)	speC	ornithine decarboxylase, constitutive
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (31/21);  total (31/21)

ATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGG  >  NC_000913/3107719‑3107996                                                                                                                                            |                                                                                                                                           aTCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAgcgc                                                                                                                                 >  1:623095/1‑151 (MQ=255) aTCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAgcgc                                                                                                                                 >  1:387732/1‑151 (MQ=255)    cgcgGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAgcgcg                                                                                                                                >  1:325853/1‑149 (MQ=255)      cgGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCa                                                                                                                              >  1:275296/1‑149 (MQ=255)       ggCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCa                                                                                                                              >  1:194321/1‑148 (MQ=255)       ggCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAg                                                                                                                            <  1:833422/150‑1 (MQ=255)       ggCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGa                                                                                                                           <  2:550830/151‑1 (MQ=255)       ggCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGa                                                                                                                           <  1:419580/151‑1 (MQ=255)       ggCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGa                                                                                                                           <  1:928637/151‑1 (MQ=255)             tCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGGTGTCGGATAATCCTGCCACAATTTGCCATCCACAACGGGCGGGATAAACGGGCGGAAACCCTTCCAGCGCGCAAGAAAAGcc                                                                                                                     >  1:282972/1‑151 (MQ=255)              ccTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGcc                                                                                                                     >  1:583845/1‑150 (MQ=255)                 tCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTg                                                                                                                  >  1:79806/1‑150 (MQ=255)                   aaaGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTgcgc                                                                                                               >  1:178049/1‑151 (MQ=255)                     aGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTgcgcgc                                                                                                             <  1:925000/151‑1 (MQ=255)                         gTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTc                                                                                                          >  1:200184/1‑150 (MQ=255)                               cTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATccc                                                                                                    <  2:736810/150‑1 (MQ=255)                               cTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATccc                                                                                                    <  2:10573/150‑1 (MQ=255)                                ttCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCt                                                                                                   >  2:924044/1‑150 (MQ=255)                                   gCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTAtct                                                                                               >  2:120449/1‑151 (MQ=255)                                      cccGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCa                                                                                             >  1:736364/1‑150 (MQ=255)                                                       aaaaCGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGc                                                                           >  2:906771/1‑151 (MQ=255)                                                         aaCGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCg                                                                         <  1:126384/151‑1 (MQ=255)                                                               ggTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGccc                                                                   >  2:897484/1‑151 (MQ=255)                                                                 tCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCAc                                                                 >  1:252155/1‑151 (MQ=255)                                                                     tGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACtctc                                                             >  1:542300/1‑151 (MQ=255)                                                                      ggCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACtctc                                                             <  2:691593/150‑1 (MQ=255)                                                                       gCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTccc                                                           <  2:141401/151‑1 (MQ=255)                                                                          aaCACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTcccc                                                          <  2:736382/149‑1 (MQ=255)                                                                          aaCACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCt                                                         <  2:427795/150‑1 (MQ=255)                                                                                gATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGa                                                   <  1:247914/150‑1 (MQ=255)                                                                                     cGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAAtttt                                              <  1:8650/150‑1 (MQ=255)                                                                                     cGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTg                                             >  1:428757/1‑151 (MQ=255)                                                                                       gATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGc                                           >  1:375807/1‑151 (MQ=255)                                                                                        aTAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCg                                          >  2:9897/1‑151 (MQ=255)                                                                                         tAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGt                                         >  1:113378/1‑151 (MQ=255)                                                                                               tGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACAt                                   >  1:855607/1‑151 (MQ=255)                                                                                                        ttGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgca                           <  2:639571/150‑1 (MQ=255)                                                                                                        ttGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgc                            >  2:846507/1‑149 (MQ=255)                                                                                                         tGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgcagc                         >  1:134744/1‑151 (MQ=255)                                                                                                          gCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgcagc                         >  2:495851/1‑150 (MQ=255)                                                                                                            cATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCaa                       >  2:658132/1‑150 (MQ=255)                                                                                                              tCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCaaa                      >  1:805563/1‑149 (MQ=255)                                                                                                                aacaacGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGc                  <  1:817647/151‑1 (MQ=255)                                                                                                                  caacGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCgg                <  2:623115/151‑1 (MQ=255)                                                                                                                   aacGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCgg                >  2:818496/1‑150 (MQ=255)                                                                                                                   aacGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGa               <  1:744392/151‑1 (MQ=255)                                                                                                                    acGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGAt              <  2:124208/151‑1 (MQ=255)                                                                                                                    acGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGAt              <  2:482324/151‑1 (MQ=255)                                                                                                                      gggcgggATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAg            <  2:470677/151‑1 (MQ=255)                                                                                                                              tAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCt    >  2:840658/1‑151 (MQ=255)                                                                                                                              tAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCt    >  2:95548/1‑151 (MQ=255)                                                                                                                                aaCGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTgg  >  2:266957/1‑151 (MQ=255)                                                                                                                                            |                                                                                                                                           ATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGG  >  NC_000913/3107719‑3107996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑