Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,140,222	T→G	I33L (ATT→CTT)	hybF ←	protein involved with the maturation of hydrogenases 1 and 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,140,222	0	T	G	100.0%	226.1 / NA	61	I33L (ATT→CTT)	hybF	protein involved with the maturation of hydrogenases 1 and 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (33/28);  total (33/28)

CCACCACCTGGCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGT  >  NC_000913/3140078‑3140365                                                                                                                                                 |                                                                                                                                                ccaccaCCTGGCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTc                                                                                                                                              <  2:411270/148‑1 (MQ=255)    ccaccTGGCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGcc                                                                                                                                         <  1:673130/150‑1 (MQ=255)    ccaccTGGCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCca                                                                                                                                        <  2:711133/151‑1 (MQ=255)     caccTGGCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCcac                                                                                                                                       >  1:432947/1‑151 (MQ=255)         tGGCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACAcgg                                                                                                                                   >  2:407157/1‑151 (MQ=255)           gCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACAcggcg                                                                                                                                 <  1:324370/151‑1 (MQ=255)              gCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTg                                                                                                                              <  1:827292/151‑1 (MQ=255)                   cccAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGAcgcg                                                                                                                         <  2:517563/151‑1 (MQ=255)                        cACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTa                                                                                                                     >  1:371594/1‑150 (MQ=255)                         aCCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTaa                                                                                                                    >  2:534349/1‑150 (MQ=255)                          ccAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAAc                                                                                                                   >  2:615893/1‑150 (MQ=255)                          ccAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACa                                                                                                                  <  2:454123/151‑1 (MQ=255)                              gCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCg                                                                                                               >  1:433792/1‑150 (MQ=255)                               ccTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGtg                                                                                                             <  1:703803/151‑1 (MQ=255)                                 tGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCg                                                                                                               >  1:353721/1‑147 (MQ=255)                                 tGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGtgc                                                                                                            >  2:421541/1‑150 (MQ=255)                                          ttATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCt                                                                                                  >  2:665169/1‑151 (MQ=255)                                            ataGACGATATGTAAATCGCACCCTTGCGCCCCCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCGCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTg                                                                                                 <  1:269914/150‑1 (MQ=255)                                            ataGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGc                                                                                                >  2:119808/1‑151 (MQ=255)                                             taGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGcc                                                                                               >  2:367461/1‑151 (MQ=255)                                               gACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGt                                                                                             >  1:232929/1‑151 (MQ=255)                                                     atGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGAt                                                                                        >  1:750872/1‑150 (MQ=255)                                                     atGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGAt                                                                                        >  1:436361/1‑150 (MQ=255)                                                     atGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATa                                                                                       <  1:275768/151‑1 (MQ=255)                                                       gTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAAt                                                                                     >  1:718946/1‑151 (MQ=255)                                                         aaaTCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAAt                                                                                     <  2:62222/149‑1 (MQ=255)                                                            tCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATACTTTCa                                                                                 >  2:68711/1‑150 (MQ=255)                                                             cGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCaa                                                                                <  2:291185/150‑1 (MQ=255)                                                                  ccTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGgcgc                                                                          <  2:803342/151‑1 (MQ=255)                                                                    ttGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGct                                                                         >  2:122236/1‑150 (MQ=255)                                                                         ccACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGc                                                                    >  2:599397/1‑150 (MQ=255)                                                                            ccGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCaaa                                                                 <  1:407142/150‑1 (MQ=255)                                                                                 ccGTGGCAGACAATTTCAAAATTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGAc                                                            >  2:850327/1‑150 (MQ=255)                                                                                 ccGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGAc                                                            >  1:134554/1‑150 (MQ=255)                                                                                  cGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACaa                                                          <  1:465172/151‑1 (MQ=255)                                                                                  cGTGGCAGACAATTTAAAAACTAAAACGGGCGGCGCTCGCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACaa                                                          <  1:57218/151‑1 (MQ=255)                                                                                   gTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACAACGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACaa                                                          >  1:651645/1‑150 (MQ=255)                                                                                     ggCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTc                                                       >  1:629931/1‑151 (MQ=255)                                                                                      gCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTc                                                       >  2:173266/1‑150 (MQ=255)                                                                                         gACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCCCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGCGACAACTCATg                                                    >  1:485160/1‑150 (MQ=255)                                                                                            aaTTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCAtt                                                >  2:849831/1‑151 (MQ=255)                                                                                               ttCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTc                                             >  1:522501/1‑151 (MQ=255)                                                                                                 cAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCg                                            <  2:529592/150‑1 (MQ=255)                                                                                                  aaaaCTAAAGCGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCg                                            <  1:538370/149‑1 (MQ=255)                                                                                                      cTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTc                                      >  2:815209/1‑151 (MQ=255)                                                                                                       tAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTcc                                     >  2:143712/1‑151 (MQ=255)                                                                                                        aaaaCGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTc                                      <  1:272641/149‑1 (MQ=255)                                                                                                        aaaaCGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCt                                    <  1:408478/151‑1 (MQ=255)                                                                                                          agcggacggCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGc                                  <  2:276627/149‑1 (MQ=255)                                                                                                          aaCGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTcc                                     >  1:814499/1‑148 (MQ=255)                                                                                                              gacggCGCTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGAcc                              <  2:789359/151‑1 (MQ=255)                                                                                                                cgccgcTCTCCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCaa                            <  2:369129/148‑1 (MQ=255)                                                                                                                      tctcCTCAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAAcagc                       >  2:184149/1‑150 (MQ=255)                                                                                                                         cctcAACGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAgg                    <  2:163105/150‑1 (MQ=255)                                                                                                                             aaCGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGgcgc                <  2:444986/150‑1 (MQ=255)                                                                                                                               cGCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGAc             <  2:43422/151‑1 (MQ=255)                                                                                                                                gCAGGAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACg            >  1:36034/1‑151 (MQ=255)                                                                                                                                      gagCGCGCCAAGTTCCAGCCTCACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGc      >  1:762053/1‑151 (MQ=255)                                                                                                                                      gagCGCGCCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTg       <  2:555447/150‑1 (MQ=255)                                                                                                                                          gcgcGAAGTTCCAGCCACGCGGGGGTGACGCGCTTAACGTTGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCgt    <  1:585722/149‑1 (MQ=255)                                                                                                                                          gcgcCAAGTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCgtgt  >  1:582191/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                CCACCACCTGGCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGT  >  NC_000913/3140078‑3140365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑