Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,204,192 T→G intergenic (‑61/+29) bacA ← / ← folB undecaprenyl pyrophosphate phosphatase/dihydroneopterin aldolase and dihydroneopterin triphosphate 2'‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,204,1920TG98.6% 264.7 / ‑3.4 72intergenic (‑61/+29)bacA/folBundecaprenyl pyrophosphate phosphatase/dihydroneopterin aldolase and dihydroneopterin triphosphate 2'‑epimerase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (32/39);  total (33/39)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.02e-01

GGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTG  >  NC_000913/3204044‑3204336
                                                                                                                                                    |                                                                                                                                                
ggCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTgat                                                                                                                                                <  1:614240/151‑1 (MQ=255)
 gCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTgat                                                                                                                                                >  2:348104/1‑150 (MQ=255)
       gCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAACCCCCGAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGGCCAGGTGTTAACCGt                                                                                                                                          <  1:637049/150‑1 (MQ=255)
        cTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGttt                                                                                                                                        >  1:601431/1‑151 (MQ=255)
          ggATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGttt                                                                                                                                        <  2:855798/149‑1 (MQ=255)
          ggATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTgg                                                                                                                                      <  1:449474/151‑1 (MQ=255)
          ggATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTgg                                                                                                                                      >  2:93484/1‑151 (MQ=255)
          ggATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTg                                                                                                                                       <  2:117525/150‑1 (MQ=255)
           gATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGt                                                                                                                                     >  2:636086/1‑151 (MQ=255)
             tACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGttt                                                                                                                                   >  1:183130/1‑151 (MQ=255)
                 ggCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACa                                                                                                                               <  1:728267/151‑1 (MQ=255)
                    aGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTTATAACCGTTTGGTTTAACAGCt                                                                                                                            >  1:859819/1‑151 (MQ=255)
                    aGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCt                                                                                                                            >  1:34941/1‑151 (MQ=255)
                    aGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCt                                                                                                                            <  1:386966/151‑1 (MQ=255)
                    aGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCt                                                                                                                            >  1:425280/1‑151 (MQ=255)
                      aaaTTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGt                                                                                                                          <  2:767944/151‑1 (MQ=255)
                      aaaTTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGt                                                                                                                          <  2:697272/151‑1 (MQ=255)
                       aaTTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCTGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTa                                                                                                                         <  2:667574/151‑1 (MQ=255)
                        aTTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTaa                                                                                                                        <  2:279702/151‑1 (MQ=255)
                          tCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTaaa                                                                                                                       >  1:857538/1‑150 (MQ=255)
                             gTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAaatta                                                                                                                   <  2:836974/151‑1 (MQ=255)
                             gTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAaatt                                                                                                                    <  1:315891/150‑1 (MQ=255)
                                  tCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGAGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAattat                                                                                                              >  2:818463/1‑151 (MQ=255)
                                  tCCTTCGACCACACCCAATACTGCCGCTATCAGCAGCAAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTGCCGTCTGTCCTAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAaattaatt                                                                                                                <  1:154920/149‑1 (MQ=255)
                                   ccTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAattat                                                                                                              <  2:274738/150‑1 (MQ=255)
                                         aCCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATtttcttt                                                                                                       >  2:788126/1‑151 (MQ=255)
                                         aCCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATtttcttt                                                                                                       <  1:400898/151‑1 (MQ=255)
                                         aCCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATtttcttt                                                                                                       <  1:916712/151‑1 (MQ=255)
                                          ccACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATtttctttc                                                                                                      <  2:50212/151‑1 (MQ=255)
                                          ccACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATtttctttc                                                                                                      >  1:428700/1‑151 (MQ=255)
                                           cacaCCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATtttctttc                                                                                                      >  2:319210/1‑150 (MQ=255)
                                             cacCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATtttctttc                                                                                                      >  2:558501/1‑148 (MQ=255)
                                                 cAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGattat                                                                                               <  2:566126/151‑1 (MQ=255)
                                                  aaTATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGattatt                                                                                              <  2:876425/151‑1 (MQ=255)
                                                     atTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGcc                                                                                           >  1:520486/1‑151 (MQ=255)
                                                       tGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCa                                                                                          <  2:753746/150‑1 (MQ=255)
                                                           gCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTc                                                                                     >  1:361649/1‑151 (MQ=255)
                                                              aTCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCa                                                                                    <  1:208309/149‑1 (MQ=255)
                                                              aTCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAAt                                                                                  <  1:182336/151‑1 (MQ=255)
                                                                         gTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCaa                                                                       <  1:35965/151‑1 (MQ=255)
                                                                          tGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAAc                                                                      >  1:474625/1‑151 (MQ=255)
                                                                               atCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTc                                                                  <  1:919156/150‑1 (MQ=255)
                                                                                 cGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTcgcc                                                               <  2:567904/151‑1 (MQ=255)
                                                                                    tcatcaATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCgccgcc                                                            >  2:838609/1‑151 (MQ=255)
                                                                                     catcaATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGccgccc                                                           <  1:221570/151‑1 (MQ=255)
                                                                                     catcaATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGccgccc                                                           >  2:44255/1‑151 (MQ=255)
                                                                                      atcaATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGccgcccg                                                          >  2:509347/1‑151 (MQ=255)
                                                                                        caATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCcgcg                                                        <  1:394001/151‑1 (MQ=255)
                                                                                         aaTAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGccgcccg                                                          >  2:694800/1‑148 (MQ=255)
                                                                                          aTAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGcc                                                      >  1:316689/1‑151 (MQ=255)
                                                                                              aCCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTg                                                  >  2:364858/1‑151 (MQ=255)
                                                                                                    aaaTTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTg                                            <  1:517103/151‑1 (MQ=255)
                                                                                                     aaTTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTgg                                           <  2:799069/151‑1 (MQ=255)
                                                                                                      attattAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGc                                          >  2:737056/1‑151 (MQ=255)
                                                                                                       ttattaAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCt                                         >  1:624729/1‑151 (MQ=255)
                                                                                                           taAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGc                                      >  2:325051/1‑150 (MQ=255)
                                                                                                            aaaaTGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTg                                    >  2:622891/1‑151 (MQ=255)
                                                                                                            aaaaTGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTg                                    >  1:494692/1‑151 (MQ=255)
                                                                                                             aaaTGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTg                                    >  2:136770/1‑150 (MQ=255)
                                                                                                              aaTGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTg                                    >  1:700234/1‑149 (MQ=255)
                                                                                                                 gTACCGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGttt                               <  2:288069/151‑1 (MQ=255)
                                                                                                                    ccGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGAt                            <  2:440073/151‑1 (MQ=255)
                                                                                                                     cGCTTGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATa                           <  1:519371/151‑1 (MQ=255)
                                                                                                                        ttGTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGc                        >  1:323376/1‑151 (MQ=255)
                                                                                                                          gTCCGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCa                       <  1:713594/150‑1 (MQ=255)
                                                                                                                             cGAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCa                   <  1:429682/151‑1 (MQ=255)
                                                                                                                              gAACTACTGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCa                   >  1:886882/1‑150 (MQ=255)
                                                                                                                                   actGCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGc               <  2:269805/149‑1 (MQ=255)
                                                                                                                                      gCGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAg            <  1:418590/149‑1 (MQ=255)
                                                                                                                                       cGTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTg         <  1:636066/151‑1 (MQ=255)
                                                                                                                                        gTATGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTg         >  2:114119/1‑150 (MQ=255)
                                                                                                                                          aTGACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGaa       <  2:766022/150‑1 (MQ=255)
                                                                                                                                            gACCAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGc     <  1:551079/150‑1 (MQ=255)
                                                                                                                                              ccAGGTGATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTg  >  2:741771/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTG  >  NC_000913/3204044‑3204336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: