Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,263,574	T→G	V29V (GTA→GTC)	tdcD ←	propionate kinase/acetate kinase C, anaerobic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,263,574	0	T	G	100.0%	256.5 / NA	69	V29V (GTA→GTC)	tdcD	propionate kinase/acetate kinase C, anaerobic
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (41/28);  total (41/28)

TGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTC  >  NC_000913/3263426‑3263719                                                                                                                                                     |                                                                                                                                                  tGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGAc                                                                                                                                                 >  2:844928/1‑151 (MQ=255)    cATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTc                                                                                                                                              <  1:98061/151‑1 (MQ=255)     aTTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTca                                                                                                                                             <  2:511845/151‑1 (MQ=255)     aTTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTc                                                                                                                                              >  2:155610/1‑150 (MQ=255)         aaaTTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAg                                                                                                                                          <  1:572953/150‑1 (MQ=255)          aaTTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTc                                                                                                                                        >  1:323992/1‑151 (MQ=255)            ttCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTc                                                                                                                                        <  2:24183/149‑1 (MQ=255)              ccGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTg                                                                                                                                    <  1:244334/151‑1 (MQ=255)                gTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGc                                                                                                                                  <  1:367002/151‑1 (MQ=255)                 tttttCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGc                                                                                                                                  >  2:337725/1‑150 (MQ=255)                         gTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCAc                                                                                                                         <  2:22683/151‑1 (MQ=255)                            cAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGGTAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACgg                                                                                                                       <  2:500362/150‑1 (MQ=255)                            cAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACgg                                                                                                                       >  1:388344/1‑150 (MQ=255)                               aTGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGaaa                                                                                                                    >  1:327732/1‑150 (MQ=255)                                  cAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACtt                                                                                                                <  1:208559/151‑1 (MQ=255)                                   aaTTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTa                                                                                                               <  1:377/151‑1 (MQ=255)                                     ttGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAAt                                                                                                             >  2:312604/1‑151 (MQ=255)                                          ttCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGa                                                                                                          >  2:355392/1‑149 (MQ=255)                                           tCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGa                                                                                                          >  2:128853/1‑148 (MQ=255)                                            cAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGAc                                                                                                      >  1:284930/1‑151 (MQ=255)                                                gCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAAc                                                                                                  <  1:343374/151‑1 (MQ=255)                                                   ccTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACATGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACcac                                                                                               >  2:554802/1‑151 (MQ=255)                                                        gTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGtt                                                                                           >  1:268049/1‑150 (MQ=255)                                                        gTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTta                                                                                          <  1:217687/151‑1 (MQ=255)                                                        gTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTta                                                                                          <  1:563753/151‑1 (MQ=255)                                                             tgtgGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAAc                                                                                     >  1:295917/1‑151 (MQ=255)                                                                   gAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAaac                                                                               <  2:395228/151‑1 (MQ=255)                                                                        aGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCg                                                                          >  1:699616/1‑151 (MQ=255)                                                                        aGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCg                                                                          >  2:733783/1‑151 (MQ=255)                                                                              gCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAat                                                                     <  2:778489/150‑1 (MQ=255)                                                                                tGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATCACGTCGGCAATACCTGACATTAAGCCTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAat                                                                     <  1:480508/148‑1 (MQ=255)                                                                                 ggCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAattca                                                                  <  2:21682/150‑1 (MQ=255)                                                                                        ccccATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCAtctc                                                          >  1:553305/1‑151 (MQ=255)                                                                                          ccATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCtt                                                        >  2:401950/1‑151 (MQ=255)                                                                                               tACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTtctc                                                   >  1:280227/1‑151 (MQ=255)                                                                                                aCGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTtctc                                                   <  2:845049/150‑1 (MQ=255)                                                                                                 cGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCAt                                                 >  2:553588/1‑151 (MQ=255)                                                                                                  ggATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATc                                                >  2:347381/1‑151 (MQ=255)                                                                                                  ggATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATc                                                >  2:483703/1‑151 (MQ=255)                                                                                                  ggATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATc                                                >  1:630828/1‑151 (MQ=255)                                                                                                    aTAAGAATGCCTTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCt                                              >  1:790917/1‑151 (MQ=255)                                                                                                    aTAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCt                                              <  1:438130/151‑1 (MQ=255)                                                                                                          aTGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTa                                        <  2:515464/151‑1 (MQ=255)                                                                                                           tGCATTTTCCGAGTTAATACAGTCGGCAATACCTGACATTAAGACTTCACAGTAGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACGGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTAc                                       >  1:482298/1‑151 (MQ=255)                                                                                                            gCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTAc                                       <  2:284938/150‑1 (MQ=255)                                                                                                            gCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACg                                      >  1:517658/1‑151 (MQ=255)                                                                                                              aTTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACgg                                     >  2:58912/1‑150 (MQ=255)                                                                                                              aTTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACgg                                     >  1:454848/1‑150 (MQ=255)                                                                                                              aTTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGa                                    <  2:295926/151‑1 (MQ=255)                                                                                                                tttCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGAtt                                  >  1:884561/1‑151 (MQ=255)                                                                                                                tttCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGAtt                                  >  1:512539/1‑151 (MQ=255)                                                                                                                 ttCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGAtt                                  >  2:582615/1‑150 (MQ=255)                                                                                                                 ttCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGAtt                                  <  1:210392/150‑1 (MQ=255)                                                                                                                  tCCGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTa                                 >  2:467989/1‑150 (MQ=255)                                                                                                                   ccGAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTaa                                <  2:527395/150‑1 (MQ=255)                                                                                                                     gAGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAAc                             >  2:226890/1‑151 (MQ=255)                                                                                                                      aGTTAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACa                            <  1:59519/151‑1 (MQ=255)                                                                                                                         tAATACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGtt                         >  1:313311/1‑151 (MQ=255)                                                                                                                            tACCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGt                      >  2:331532/1‑151 (MQ=255)                                                                                                                             aCCGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGta                     <  2:686222/151‑1 (MQ=255)                                                                                                                               cGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGtat                    >  2:526702/1‑150 (MQ=255)                                                                                                                               cGTCGGCAATACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGtat                    >  1:722010/1‑150 (MQ=255)                                                                                                                                      aaTACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGtt            <  2:621389/151‑1 (MQ=255)                                                                                                                                        tACCTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTc           >  2:445429/1‑150 (MQ=255)                                                                                                                                           cTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAgg        >  2:537445/1‑150 (MQ=255)                                                                                                                                           cTGACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAgg        >  2:221461/1‑150 (MQ=255)                                                                                                                                             gACATTAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGAt      <  2:149989/150‑1 (MQ=255)                                                                                                                                                 ttAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTc  >  1:903709/1‑150 (MQ=255)                                                                                                                                                  tAAGACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTc  >  1:624459/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                  TGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTC  >  NC_000913/3263426‑3263719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑