Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,334,208	A→C	I167L (ATC→CTC)	ispB →	octaprenyl diphosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,334,208	0	A	C	100.0%	185.2 / NA	49	I167L (ATC→CTC)	ispB	octaprenyl diphosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (20/29);  total (20/29)

CGCTTTCCAGATGATGACCAGCCTCGGTTCGCTCAAAGTGCTGGAAGTCATGTCAGAAGCCGTAAACGTCATCGCAGAAGGTGAAGTTCTGCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCG  >  NC_000913/3334063‑3334352                                                                                                                                                  |                                                                                                                                                 cgcTTTCCAGATGATGACCAGCCTCGGTTCGCTCAAAGTGCTGGAAGTCATGTCAGAAGCCGTAAACGTCATCGCAGAAGGTGAAGTTCTGCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCtat                                                                                                                                             <  1:916500/151‑1 (MQ=255) cgcTTTCCAGATGATGACCAGCCTCGGTTCGCTCAAAGTGCTGGAAGTCATGTCAGAAGCCGTAAACGTCATCGCAGAAGGTGAAGTTCTGCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCtat                                                                                                                                             >  1:451862/1‑151 (MQ=255)  gcTTTCCAGATGATGACCAGCCTCGGTTCGCTCAAAGTGCTGGAAGTCATGTCAGAAGCCGTAAACGTCATCGCAGAAGGTGAAGTTCTGCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCtata                                                                                                                                            <  2:242277/151‑1 (MQ=255)       ccAGATGATGACCAGCCTCGGTTCGCTCAAAGTGCTGGAAGTCATGTCAGAAGCCGTAAACGTCATCGCAGAAGGTGAAGTTCTGCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCaa                                                                                                                                        <  1:53168/150‑1 (MQ=255)         aGATGATGACCAGCCTCGGTTCGCTCAAAGTGCTGGAAGTCATGTCAGAAGCCGTAAACGTCATCGCAGAAGGTGAAGTTCTGCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAAc                                                                                                                                     <  1:640971/151‑1 (MQ=255)           atgatgACCAGCCTCGGTTCGCTCAAAGTGCTGGAAGTCATGTCAGAAGCCGTAAACGTCATCGCAGAAGGTGAAGTTCTGCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACcg                                                                                                                                   <  1:504682/151‑1 (MQ=255)           atgatgACCAGCCTCGGTTCGCTCAAAGTGCTGGAAGTCATGTCAGAAGCCGTAAACGTCATCGCAGAAGGTGAAGTTCTGCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGAAAAACcg                                                                                                                                   <  2:340462/151‑1 (MQ=255)                      ccTCGGTTCGCTCAAAGTGCTGGAAGTCATGTCAGAAGCCGTAAACGTCATCGCAGAAGGTGAAGTTCTGCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGttt                                                                                                                        <  2:625917/151‑1 (MQ=255)                       cTCGGTTCGCTCAAAGTGCTGGAAGTCATGTCAGAAGCCGTAAACGTCATCGCAGAAGGTGAAGTTCTGCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTg                                                                                                                       >  1:453320/1‑151 (MQ=255)                       cTCGGTTCGCTCAAAGTGCTGGAAGTCATGTCAGAAGCCGTAAACGTCATCGCAGAAGGTGAAGTTCTGCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTg                                                                                                                       >  1:505403/1‑151 (MQ=255)                                cTCAAAGTGCTGGAAGTCATGTCAGAAGCCGTAAACGTCATCGCAGAAGGTGAAGTTCTGCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGc                                                                                                                >  1:199702/1‑149 (MQ=255)                                           ggAAGTCATGTCAGAAGCCGTAAACGTCATCGCAGAAGGTGAAGTTCTGCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTc                                                                                                    <  2:526861/150‑1 (MQ=255)                                              aGTCATGTCAGAAGCCGTAAACGTCATCGCAGAAGGTGAAGTTCTGCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCggg                                                                                                >  1:200890/1‑151 (MQ=255)                                                       aGAAGCCGTAAACGTCATCGCAGAAGGTGAAGTTCTGCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTctggct                                                                                       <  1:212015/151‑1 (MQ=255)                                                         aaGCCGTAAACGTCATCGCAGAAGGTGAAGTTCTGCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTctggctgg                                                                                     >  1:779026/1‑151 (MQ=255)                                                          aGCCGTAAACGTCATCGCAGAAGGTGAAGTTCTGCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCtggctggc                                                                                    <  1:58445/151‑1 (MQ=255)                                                          aGCCGTAAACGTCATCGCAGAAGGTGAAGTTCTGCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCtggctggc                                                                                    >  2:764820/1‑151 (MQ=255)                                                              gTAAACGTCATCGCAGAAGGTGAAGTTCTGCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTa                                                                                <  2:896099/151‑1 (MQ=255)                                                               tAAACGTCATCGCAGAAGGTGAAGTTCTGCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTa                                                                                <  2:219676/150‑1 (MQ=255)                                                                  aCGTCATCGCAGAAGGTGAAGTTCTGCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGcc                                                                            <  1:227631/151‑1 (MQ=255)                                                                    gTCATCGCAGAAGGTGAAGTTCTGCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCgg                                                                          <  2:618649/151‑1 (MQ=255)                                                                         cGCAGAAGGTGAAGTTCTGCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCggagg                                                                       >  2:520431/1‑149 (MQ=255)                                                                           cAGAAGGTGAAGTTCTGCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCggaggagga                                                                   <  1:683407/151‑1 (MQ=255)                                                                                     aGTTCTGCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTg                                                         <  2:657454/151‑1 (MQ=255)                                                                                       ttCTGCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGc                                                        >  2:452554/1‑150 (MQ=255)                                                                                           gCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAg                                                      <  2:810898/148‑1 (MQ=255)                                                                                           gCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGAt                                                   >  2:180443/1‑151 (MQ=255)                                                                                              aCTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTAt                                                <  1:583615/151‑1 (MQ=255)                                                                                                     aaCGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCt                                         <  2:451877/151‑1 (MQ=255)                                                                                                      aCGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTa                                        <  1:412856/151‑1 (MQ=255)                                                                                                      aCGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTa                                        <  2:420486/151‑1 (MQ=255)                                                                                                          tAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTAtctc                                    >  2:449535/1‑151 (MQ=255)                                                                                                           aaCGATCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTAtctc                                    <  1:796048/150‑1 (MQ=255)                                                                                                               aTCCGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCAc                               >  2:49233/1‑151 (MQ=255)                                                                                                                 ccGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTg                             <  1:576085/151‑1 (MQ=255)                                                                                                                 ccGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTg                             <  2:412141/151‑1 (MQ=255)                                                                                                                  cGGACATCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGc                            >  2:787831/1‑151 (MQ=255)                                                                                                                       aTCACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTcc                       >  2:635077/1‑151 (MQ=255)                                                                                                                         cACTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTcc                       >  2:162798/1‑149 (MQ=255)                                                                                                                          aCTGAAGAAAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGt                    >  1:860590/1‑151 (MQ=255)                                                                                                                              aagaaAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGAt                >  1:763040/1‑151 (MQ=255)                                                                                                                               agaaAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATc               <  2:49442/151‑1 (MQ=255)                                                                                                                               agaaAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATc               <  1:211329/151‑1 (MQ=255)                                                                                                                                gaaAACTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATcg              >  2:709831/1‑151 (MQ=255)                                                                                                                                   aaCTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATcgacg           <  2:579612/151‑1 (MQ=255)                                                                                                                                    aCTACATGCGCGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATcgacga          <  1:628111/151‑1 (MQ=255)                                                                                                                                           gcgcGTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACt    <  2:609366/150‑1 (MQ=255)                                                                                                                                            cgcgTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCg  >  2:655025/1‑151 (MQ=255)                                                                                                                                             gcgTTCTCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCg  >  2:243144/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                 CGCTTTCCAGATGATGACCAGCCTCGGTTCGCTCAAAGTGCTGGAAGTCATGTCAGAAGCCGTAAACGTCATCGCAGAAGGTGAAGTTCTGCAACTGATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCG  >  NC_000913/3334063‑3334352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑