Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,400,678 T→G E137A (GAA→GCA)  mreB ← cell wall structural complex MreBCD, actin‑like component MreB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,400,6780TG98.2% 200.1 / NA 57E137A (GAA→GCA) mreBcell wall structural complex MreBCD, actin‑like component MreB
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (30/26);  total (30/27)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

AGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTT  >  NC_000913/3400530‑3400825
                                                                                                                                                    |                                                                                                                                                   
agaagaGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCa                                                                                                                                                   <  2:78312/151‑1 (MQ=255)
 gaagaGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCAc                                                                                                                                                  >  2:597309/1‑151 (MQ=255)
  aagaGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCAc                                                                                                                                                  >  1:154932/1‑150 (MQ=255)
  aagaGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACg                                                                                                                                                 <  1:848219/151‑1 (MQ=255)
   agagGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACgg                                                                                                                                                <  1:837043/151‑1 (MQ=255)
               cacaCCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGccc                                                                                                                                    <  2:222349/151‑1 (MQ=255)
                  acCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTgc                                                                                                                                 >  1:361843/1‑151 (MQ=255)
                     gTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGcgcg                                                                                                                              >  1:614285/1‑151 (MQ=255)
                      ttCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCgg                                                                                                                             >  1:484758/1‑151 (MQ=255)
                       tCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCgg                                                                                                                             >  2:251057/1‑150 (MQ=255)
                                   cAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTgc                                                                                                                >  2:196144/1‑151 (MQ=255)
                                    aGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTgcg                                                                                                               <  2:676538/151‑1 (MQ=255)
                                       aaCTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCgcgg                                                                                                            <  2:326953/151‑1 (MQ=255)
                                       aaCTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCgcgg                                                                                                            <  2:481666/151‑1 (MQ=255)
                                       aaCTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCgcgg                                                                                                            >  1:5878/1‑151 (MQ=255)
                                           tCAGTGGTACCACCACCGATATCATCCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGTAAGACTGCCCGGGCACCAGCGCCCTGCGCGAATTCACGAATTGCgcggcg                                                                                                          <  2:773882/149‑1 (MQ=255)
                                           tCAGTGGTACCACCACCGATATCAACCCCCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCGCCAGCGCCCTGCGCGGATTCACGAATTGCgcggcg                                                                                                          <  2:407229/149‑1 (MQ=255)
                                           tCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGt                                                                                                         <  2:552751/150‑1 (MQ=255)
                                            cAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTc                                                                                                       >  2:619590/1‑151 (MQ=255)
                                                   accaccaccGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTgg                                                                                                >  2:295773/1‑151 (MQ=255)
                                                    ccaccaccGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTggg                                                                                               >  1:643935/1‑151 (MQ=255)
                                                          ccGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCgcgc                                                                                         >  1:321314/1‑151 (MQ=255)
                                                            gATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGcc                                                                                        >  1:438834/1‑150 (MQ=255)
                                                            gATATCAACCACCACAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTCCGGCAGCCATCGGTTCTGCAATCAGGAAGACTGCACGGGCTCCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTTGGTCGCGCCa                                                                                       <  1:458179/151‑1 (MQ=255)
                                                               atCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAAcc                                                                                    <  2:614183/151‑1 (MQ=255)
                                                               atCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAAc                                                                                     <  2:402747/150‑1 (MQ=255)
                                                                tCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCg                                                                                   >  1:157871/1‑151 (MQ=255)
                                                                   accaccATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGc                                                                                 >  1:45160/1‑150 (MQ=255)
                                                                    ccaccaTAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGcac                                                                               >  1:224005/1‑151 (MQ=255)
                                                                       ccaTAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGcacaca                                                                            <  2:309228/151‑1 (MQ=255)
                                                                                 ggTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAAcg                                                                  >  1:422284/1‑151 (MQ=255)
                                                                                 ggTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAAcg                                                                  <  1:276564/151‑1 (MQ=255)
                                                                                    cGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAAcgcgc                                                               <  1:865359/151‑1 (MQ=255)
                                                                                       ttCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCggg                                                            >  1:217830/1‑151 (MQ=255)
                                                                                               ccGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGAc                                                     >  1:892474/1‑150 (MQ=255)
                                                                                                cGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGc                                                   <  2:819988/151‑1 (MQ=255)
                                                                                                cGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGc                                                   <  2:856091/151‑1 (MQ=255)
                                                                                                   cAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCAt                                                 >  2:433519/1‑150 (MQ=255)
                                                                                                    aGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCAAACAAACCAGAACGCGCGGGCTTGGACGCAt                                                 >  1:929364/1‑149 (MQ=255)
                                                                                                        cAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCt                                           <  1:178467/151‑1 (MQ=255)
                                                                                                            aCCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTg                                       <  2:460094/151‑1 (MQ=255)
                                                                                                            aCCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTg                                       >  2:640680/1‑151 (MQ=255)
                                                                                                             ccAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGc                                      >  1:158909/1‑151 (MQ=255)
                                                                                                              cAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGc                                      <  1:206067/150‑1 (MQ=255)
                                                                                                              cAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCt                                     <  1:214477/151‑1 (MQ=255)
                                                                                                                   gCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGc                                 >  2:39103/1‑150 (MQ=255)
                                                                                                                   gCGGCAGCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCa                                <  1:752631/151‑1 (MQ=255)
                                                                                                                         gCCATCGGTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGtt                          >  1:25745/1‑151 (MQ=255)
                                                                                                                               ggTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTtgatg                     >  2:165376/1‑150 (MQ=255)
                                                                                                                                gTTCTTCAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTtgatga                    >  2:507339/1‑150 (MQ=255)
                                                                                                                                  tcttcAATCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAg                  <  1:640240/150‑1 (MQ=255)
                                                                                                                                        aTCAGGAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTgg            >  2:387570/1‑150 (MQ=255)
                                                                                                                                            ggAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCAt       <  2:224010/151‑1 (MQ=255)
                                                                                                                                            ggAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGCGCACTTGTTTGATGAAGTGCTGGAGCAt       <  2:836530/151‑1 (MQ=255)
                                                                                                                                            ggAAGACCGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCAt       >  1:136046/1‑151 (MQ=255)
                                                                                                                                             gAAGACTGCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCAtt      >  1:140923/1‑151 (MQ=255)
                                                                                                                                                 aCTGCACGGGCACCAGCGCCCTGCGGGGATTCACGAATTGCGCGGCGTTGAACCTGGGTCAAGACAACCGAAACATAAACCAAACCGAGCGGGCTTGGACGCATAAAGCTGTTACTGTACACTTGTTTGTTGAAGTGCTGGAGCAtttttt  <  1:497511/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTT  >  NC_000913/3400530‑3400825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑