Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,476,689	T→G	E244A (GAG→GCG)	fkpA ←	FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,476,689	0	T	G	100.0%	212.2 / NA	59	E244A (GAG→GCG)	fkpA	FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (35/24);  total (35/24)

ATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCC  >  NC_000913/3476552‑3476835                                                                                                                                          |                                                                                                                                                   atatCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAacac                                                                                                                                       <  2:913865/151‑1 (MQ=255) atatCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAacac                                                                                                                                       >  1:669853/1‑151 (MQ=255)   atCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACAcc                                                                                                                                      >  2:831758/1‑150 (MQ=255)        gCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTa                                                                                                                                <  1:201617/151‑1 (MQ=255)              aataaAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTc                                                                                                                          <  1:317889/151‑1 (MQ=255)                 aaaaaaaCCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGt                                                                                                                       <  1:870259/151‑1 (MQ=255)                  aaaaaaCCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTg                                                                                                                      <  1:411400/151‑1 (MQ=255)                     aaaCCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGa                                                                                                                   <  1:791950/151‑1 (MQ=255)                      aaCCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGAt                                                                                                                  >  2:763517/1‑151 (MQ=255)                      aaCCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGAt                                                                                                                  <  2:869752/151‑1 (MQ=255)                          gccgccTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATcccc                                                                                                              >  1:668988/1‑151 (MQ=255)                           ccgccTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCg                                                                                                             >  1:693801/1‑151 (MQ=255)                                tGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAac                                                                                                         >  1:342160/1‑150 (MQ=255)                                  gTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACAcc                                                                                                      <  1:611791/151‑1 (MQ=255)                                          gcggTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGc                                                                                              >  2:331469/1‑151 (MQ=255)                                          gcggTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGc                                                                                              >  1:223506/1‑151 (MQ=255)                                            ggTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCg                                                                                            >  1:329063/1‑151 (MQ=255)                                            ggTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCg                                                                                            >  1:270113/1‑151 (MQ=255)                                             gTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTACCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGt                                                                                           <  1:763897/151‑1 (MQ=255)                                              ttCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGt                                                                                           >  1:3598/1‑150 (MQ=255)                                              ttCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGt                                                                                           >  1:216357/1‑150 (MQ=255)                                                  tAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTaa                                                                                         <  1:922040/148‑1 (MQ=255)                                                  tAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTaa                                                                                         <  2:726307/148‑1 (MQ=255)                                                  tAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTaa                                                                                         <  2:108435/148‑1 (MQ=255)                                                  tAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAg                                                                                        <  2:878132/149‑1 (MQ=255)                                                  tAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGcc                                                                                      >  2:723999/1‑151 (MQ=255)                                                      gCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGt                                                                                   <  1:904598/150‑1 (MQ=255)                                                         tATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCtg                                                                               >  2:680116/1‑151 (MQ=255)                                                               ttAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGa                                                                          >  2:805835/1‑150 (MQ=255)                                                                     gAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCa                                                                   <  2:366376/151‑1 (MQ=255)                                                                      aaTCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAg                                                                  <  2:296960/151‑1 (MQ=255)                                                                       aTCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGt                                                                 >  1:267429/1‑151 (MQ=255)                                                                           gCGGCTTTCGCATCAGCTTCAGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTg                                                              >  1:477175/1‑150 (MQ=255)                                                                                 ttCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTAc                                                       >  1:819793/1‑151 (MQ=255)                                                                                 ttCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTAc                                                       >  2:154484/1‑151 (MQ=255)                                                                                    gCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGc                                                    >  2:263414/1‑151 (MQ=255)                                                                                         aGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTc                                               >  2:12018/1‑151 (MQ=255)                                                                                           cTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCt                                              >  2:518933/1‑150 (MQ=255)                                                                                            ttCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCTTg                                            >  2:485508/1‑151 (MQ=255)                                                                                             tCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCTTg                                            >  2:76131/1‑150 (MQ=255)                                                                                              ccGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCTTGAt                                          <  1:612414/151‑1 (MQ=255)                                                                                                   tttGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCTTGATGttct                                     >  1:702503/1‑151 (MQ=255)                                                                                                   tttGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCTTGATGttct                                     >  1:764227/1‑151 (MQ=255)                                                                                                     tGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCTTGATGttcttc                                   >  2:17082/1‑151 (MQ=255)                                                                                                      gCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCa                                  <  2:213985/151‑1 (MQ=255)                                                                                                        aTCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCTTGATGttcttc                                   >  2:908632/1‑148 (MQ=255)                                                                                                        aTCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGa                                <  2:669872/151‑1 (MQ=255)                                                                                                        aTCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGGAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCTTGATGctc                                      >  1:486852/1‑145 (MQ=255)                                                                                                         tCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGAc                               >  1:565150/1‑151 (MQ=255)                                                                                                          cAGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGAc                               <  2:563468/150‑1 (MQ=255)                                                                                                           aGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGACCt                             <  2:608571/151‑1 (MQ=255)                                                                                                           aGCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGACCt                             <  1:235458/151‑1 (MQ=255)                                                                                                            gCCTTCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGACCtt                            <  1:455584/151‑1 (MQ=255)                                                                                                                tCGGCGCTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTg                         <  1:210099/150‑1 (MQ=255)                                                                                                                     gcTGGTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAAc                   >  2:200863/1‑151 (MQ=255)                                                                                                                        ggTTTCACATCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCg                >  1:560746/1‑151 (MQ=255)                                                                                                                              acaTCCAGCAGCGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATa           >  2:641551/1‑150 (MQ=255)                                                                                                                                    agcagcGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGt    >  2:615471/1‑151 (MQ=255)                                                                                                                                       agcGCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTcc  >  2:369578/1‑150 (MQ=255)                                                                                                                                          |                                                                                                                                                   ATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCC  >  NC_000913/3476552‑3476835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑