Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,554,067	T→G	L328R (CTG→CGG)	malT →	mal regulon transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,554,067	0	T	G	100.0%	246.2 / NA	67	L328R (CTG→CGG)	malT	mal regulon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (34/33);  total (34/33)

TCAATGAACGATGCCCTCATCACCCGTGTGACCGGCGAAGAAAACGGGCAAATGCGCCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTC  >  NC_000913/3553919‑3554213                                                                                                                                                     |                                                                                                                                                   tCAATGAACGATGCCCTCATCACCCGTGTGACCGGCGAAGAAAACGGGCAAATGCGCCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGgc                                                                                                                                                  >  2:817009/1‑151 (MQ=255)  cAATGAACGATGCCCTCATCACCCGTGTGACCGGCGAAGAAAACGGGCAAATGCGCCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGgcg                                                                                                                                                 >  1:23105/1‑151 (MQ=255)    aTGAACGATGCCCTCATCACCCGTGTGACCGGCGAAGAAAACGGGCAAATGCGCCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGcc                                                                                                                                               >  1:481410/1‑151 (MQ=255)        aCGATGCCCTCATCACCCGTGTGACCGGCGAAGAAAACGGGCAAATGCGCCTCGAAGAGATTGAGCGTCAGGAGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAgcg                                                                                                                                           <  2:82234/151‑1 (MQ=255)           aTGCCCTCATCACCCGTGTGACCGGCGAAGAAAACGGGCAAATGCGCCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAgcgc                                                                                                                                          >  1:299691/1‑149 (MQ=255)              cccTCATCACCCGTGTGACCGGCGAAGAAAACGGGCAAATGCGCCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGcc                                                                                                                                      >  1:168196/1‑150 (MQ=255)              cccTCATCACCCGTGTGACCGGCGAAGAAAACGGGCAAATGCGCCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCa                                                                                                                                     <  1:312798/151‑1 (MQ=255)                 tcatcaCCCGTGTGACCGGCGAAGAAAACGGGCAAATGCGCCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCGCCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTg                                                                                                                                  >  1:589379/1‑151 (MQ=255)                   atcaCCCGTGTGACCGGCGAAGAAAACGGGCAAATGCGCCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTggg                                                                                                                                >  1:861318/1‑151 (MQ=255)                    tcaCCCGTGTGACCGGCGAAGAAAACGGGCAAATGCGCCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTggg                                                                                                                                >  1:400113/1‑150 (MQ=255)                     caCCCGTGTGACCGGCGAAGAAAACGGGCAAATGCGCCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGaa                                                                                                                              <  2:574369/151‑1 (MQ=255)                     caCCCGTGTGACCGGCGAAGAAAACGGGCAAATGCGCCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGa                                                                                                                               >  1:139696/1‑150 (MQ=255)                     caCCCGTGTGACCGGCGAAGAAAACGGGCAAATGCGCCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGa                                                                                                                               >  1:400440/1‑150 (MQ=255)                      aCCCGTGTGACCGGCGAAGAAAACGGGCAAATGCGCCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAAc                                                                                                                             >  1:378310/1‑151 (MQ=255)                          gtgtGACCGGCGAAGAAAACGGGCAAATGCGCCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTggc                                                                                                                         <  2:797125/151‑1 (MQ=255)                            gtgACCGGCGAAGAAAACGGGCAAATGCGCCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTggcg                                                                                                                        <  2:111583/150‑1 (MQ=255)                              gACCGGCGAAGAAAACGGGCAAATGCGCCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTggcggc                                                                                                                      <  1:383522/150‑1 (MQ=255)                                ccGGCGAAGAAAACGGGCAAATGCGCCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGa                                                                                                                   <  1:93266/151‑1 (MQ=255)                                 cGGCGAAGAAAACGGGCAAATGCGCCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAg                                                                                                                  <  1:533366/151‑1 (MQ=255)                                  ggCGAAGAAAACGGGCAAATGCGCCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGc                                                                                                                 <  2:470990/151‑1 (MQ=255)                                   gCGAAGAAAACGGGCAAATGCGCCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCt                                                                                                                <  1:424870/151‑1 (MQ=255)                                      aagaaAACGGGCAAATGCGCCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGcc                                                                                                             >  1:352693/1‑151 (MQ=255)                                       agaaAACGGGCAAATGCGCCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGc                                                                                                              <  1:19349/149‑1 (MQ=255)                                          aaaCGGGCAAATGCGCCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCgg                                                                                                           >  1:528786/1‑149 (MQ=255)                                          aaaCGGGCAAATGCGCCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGaa                                                                                                         <  2:845525/151‑1 (MQ=255)                                             cGGGCAAATGCGCCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATc                                                                                                      <  2:852588/151‑1 (MQ=255)                                                    aTGCGCCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCg                                                                                                 <  2:395343/149‑1 (MQ=255)                                                        gcCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTgccgc                                                                                           >  2:303672/1‑151 (MQ=255)                                                           tCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCcgccgc                                                                                        >  1:157532/1‑151 (MQ=255)                                                            cGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGccgccg                                                                                         <  2:6419/149‑1 (MQ=255)                                                               agagATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGa                                                                                     >  2:379528/1‑150 (MQ=255)                                                                  gaTTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGc                                                                                 >  2:493963/1‑151 (MQ=255)                                                                   aTTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCt                                                                                <  2:162899/151‑1 (MQ=255)                                                                      gAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTgg                                                                              >  1:492735/1‑150 (MQ=255)                                                                              ggggCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGccc                                                                       >  2:492597/1‑149 (MQ=255)                                                                                ggCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAggg                                                                   >  2:82330/1‑151 (MQ=255)                                                                                ggCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAgg                                                                    >  2:404486/1‑150 (MQ=255)                                                                                    gTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGAtt                                                                <  1:376695/150‑1 (MQ=255)                                                                                     tttttACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTc                                                              >  2:133044/1‑151 (MQ=255)                                                                                      ttttACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTcc                                                             >  2:848952/1‑151 (MQ=255)                                                                                       tttACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTccc                                                            >  2:201444/1‑151 (MQ=255)                                                                                       tttACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTcc                                                             <  2:190080/150‑1 (MQ=255)                                                                                         tACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCa                                                           <  2:201374/150‑1 (MQ=255)                                                                                          aCAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAg                                                          <  1:17351/150‑1 (MQ=255)                                                                                          aCAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGc                                                         >  2:388402/1‑151 (MQ=255)                                                                                             gCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGa                                                       >  2:367815/1‑150 (MQ=255)                                                                                              cGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGa                                                       >  2:546460/1‑149 (MQ=255)                                                                                               ggatggatGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGc                                                    <  1:701045/151‑1 (MQ=255)                                                                                                 atggatgATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCa                                                   >  1:133953/1‑150 (MQ=255)                                                                                                   ggatgATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAAtt                                                <  1:344398/151‑1 (MQ=255)                                                                                                          aCCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATg                                         <  2:444377/151‑1 (MQ=255)                                                                                                           ccGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATg                                         <  2:826668/150‑1 (MQ=255)                                                                                                            cGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATgcg                                       <  2:427094/151‑1 (MQ=255)                                                                                                                gAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTg                                    <  1:607615/150‑1 (MQ=255)                                                                                                                 aGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTggc                                  <  2:136000/151‑1 (MQ=255)                                                                                                                    ggTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTggcgg                                <  1:841088/150‑1 (MQ=255)                                                                                                                     gTTCTGCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTggcggc                               >  1:306271/1‑150 (MQ=255)                                                                                                                          gCTATCACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCg                          <  2:823002/150‑1 (MQ=255)                                                                                                                               cACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATgcgc                    <  1:559756/151‑1 (MQ=255)                                                                                                                               cACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATgcgc                    >  2:253876/1‑151 (MQ=255)                                                                                                                               cACCCGCTGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATgcgc                    <  2:258999/151‑1 (MQ=255)                                                                                                                                      tGTTTGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGAtgct             >  1:586816/1‑151 (MQ=255)                                                                                                                                        tttGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGAtgctgc           >  2:897235/1‑151 (MQ=255)                                                                                                                                          tGGTAACTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGTCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTgcg          <  1:433100/150‑1 (MQ=255)                                                                                                                                              aaCTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGat      >  2:372840/1‑150 (MQ=255)                                                                                                                                                cTTCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGatat    <  1:682518/150‑1 (MQ=255)                                                                                                                                                 ttCCGGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTc  >  2:510252/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                   TCAATGAACGATGCCCTCATCACCCGTGTGACCGGCGAAGAAAACGGGCAAATGCGCCTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTC  >  NC_000913/3553919‑3554213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑