Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,657,161	T→G	L56* (TTA→TGA)	hdeD →	acid‑resistance membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,657,161	0	T	G	100.0%	269.6 / NA	72	L56* (TTA→TGA)	hdeD	acid‑resistance membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (31/41);  total (31/41)

GCAACAATTTTGAAGTTTGATCTGGAGATGCTTAAAAAACATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATT  >  NC_000913/3657013‑3657306                                                                                                                                                     |                                                                                                                                                  gCAACAATTTTGAAGTTTGATCTGGAGATGCTTAAAAAACATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCatgat                                                                                                                                                 >  1:279731/1‑151 (MQ=255)    acaaTTTTGAAGTTTGATCTGGAGATGCTTAAAAAACATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCAtgatta                                                                                                                                               >  1:916750/1‑150 (MQ=255)     caaTTTTGAAGTTTGATCTGGAGATGCTTAAAAAACATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAAt                                                                                                                                             <  1:580493/151‑1 (MQ=255)       aTTTTGAAGTTTGATCTGGAGATGCTTAAAAAACATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCt                                                                                                                                           <  2:235298/151‑1 (MQ=255)       aTTTTGAAGTTTGATCTGGAGATGCTTAAAAAACATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGGTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGTTATTTTAAGCACAGTAGTGGGTGCATGATTAATCt                                                                                                                                           >  2:284093/1‑151 (MQ=255)          ttGAAGTTTGATCTGGAGATGCTTAAAAAACATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGc                                                                                                                                         >  2:932705/1‑150 (MQ=255)            gAAGTTTGATCTGGAGATGCTTAAAAAACATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTc                                                                                                                                       <  2:546291/150‑1 (MQ=255)            gAAGTTTGATCTGGAGATGCTTAAAAAACATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCg                                                                                                                                      >  2:674492/1‑151 (MQ=255)                 ttGATCTGGAGATGCTTAAAAAACATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTa                                                                                                                                  <  1:162466/150‑1 (MQ=255)                 ttGATCTGGAGATGCTTAAAAAACATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTAt                                                                                                                                 >  2:583706/1‑151 (MQ=255)                  tGATCTGGAGATGCTTAAAAAACATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGGGCATGATTAATCTGCTCGGGTAtt                                                                                                                                >  2:918818/1‑151 (MQ=255)                   gATCTGGAGATGCTTAAAAAACATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTAtt                                                                                                                                <  1:181647/150‑1 (MQ=255)                       tGGAGATGCTTAAAAAACATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCt                                                                                                                           >  2:759267/1‑151 (MQ=255)                        ggAGATGCTTAAAAAACATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTgc                                                                                                                              <  2:929708/147‑1 (MQ=255)                        ggAGATGCTTAAAAAACATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTgc                                                                                                                              <  1:307054/147‑1 (MQ=255)                        ggAGATGCTTAAAAAACATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTgc                                                                                                                              <  2:334837/147‑1 (MQ=255)                        ggAGATGCTTAAAAAACATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTgc                                                                                                                              <  2:353178/147‑1 (MQ=255)                        ggAGATGCTTAAAAAACATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTg                                                                                                                               <  1:418392/146‑1 (MQ=255)                        ggAGATGCTTAAAAAACATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCtt                                                                                                                          >  1:840532/1‑151 (MQ=255)                        ggAGATGCTTAAAAAACATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCt                                                                                                                           <  2:571065/150‑1 (MQ=255)                           gaTGCTTAAAAAACATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTAtt                                                                                                                       <  2:529513/151‑1 (MQ=255)                              gCTTAAAAAACATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTc                                                                                                                    >  1:365866/1‑151 (MQ=255)                                 tAAAAAACATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCggg                                                                                                                 >  2:428146/1‑151 (MQ=255)                                 tAAAAAACATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCgg                                                                                                                  <  2:585877/150‑1 (MQ=255)                                   aaaaaCATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGtt                                                                                                               <  2:90674/151‑1 (MQ=255)                                    aaaaCATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTa                                                                                                              <  2:912803/151‑1 (MQ=255)                                       aCATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTc                                                                                                           <  1:812662/151‑1 (MQ=255)                                           cGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGc                                                                                                        >  1:35151/1‑150 (MQ=255)                                            gCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATGGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCAAGCTTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCa                                                                                                          >  1:702837/1‑147 (MQ=255)                                              agagCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAAcc                                                                                                    >  1:224013/1‑151 (MQ=255)                                                  cAACCCAGTTTATTGCCGTGCTGCTGTTTATCGTTGGGTTGCTGTGTATCAGTTTCCCGTGCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCgg                                                                                                  <  1:438338/149‑2 (MQ=255)                                                   aaTCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAg                                                                                                >  1:813346/1‑150 (MQ=255)                                                        aGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATaa                                                                                          <  1:788821/151‑1 (MQ=255)                                                        aGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATaa                                                                                          <  1:664788/151‑1 (MQ=255)                                                        aGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATa                                                                                           >  2:50169/1‑150 (MQ=255)                                                         gTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAAt                                                                                         >  2:782408/1‑151 (MQ=255)                                                            tATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAAttt                                                                                       >  1:118005/1‑150 (MQ=255)                                                             aTTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTc                                                                                      <  1:382870/150‑1 (MQ=255)                                                                        gctgTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGtatta                                                                          <  1:695524/151‑1 (MQ=255)                                                                        gctgTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGtatt                                                                           <  1:427640/150‑1 (MQ=255)                                                                        gctgTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGtat                                                                            <  2:437845/149‑1 (MQ=255)                                                                           gTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGtattat                                                                         <  1:923268/149‑1 (MQ=255)                                                                                 cgtcgGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTc                                                                 >  1:809201/1‑151 (MQ=255)                                                                                 cgtcgGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTc                                                                 >  2:531974/1‑151 (MQ=255)                                                                                  gtcgGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTcc                                                                >  1:197538/1‑151 (MQ=255)                                                                                     gggTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCtcg                                                             >  1:35863/1‑151 (MQ=255)                                                                                      ggTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCtcgt                                                            <  1:153292/151‑1 (MQ=255)                                                                                      ggTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCtcgt                                                            >  2:465044/1‑151 (MQ=255)                                                                                       gTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCtcgtc                                                           <  2:330638/151‑1 (MQ=255)                                                                                                   cAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCta                                                <  1:571917/150‑1 (MQ=255)                                                                                                        tCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCtatttatt                                          >  2:858528/1‑151 (MQ=255)                                                                                                        tCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCtatttat                                           <  2:841115/150‑1 (MQ=255)                                                                                                            gTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTAATAATTTATGGCCGGTATTCTCCGGTTTCCTCGTCGCAGCAGCCTATTTATTGAt                                       >  1:27597/1‑150 (MQ=255)                                                                                                                   tctGGCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTAtt                               >  1:66119/1‑151 (MQ=255)                                                                                                                      ggCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTAttt                              <  2:46598/149‑1 (MQ=255)                                                                                                                      ggCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATttct                            <  1:141551/151‑1 (MQ=255)                                                                                                                      ggCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATttct                            <  1:699611/151‑1 (MQ=255)                                                                                                                      ggCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATttc                             <  1:348429/150‑1 (MQ=255)                                                                                                                       gCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATttctt                           <  2:118733/151‑1 (MQ=255)                                                                                                                       gCGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATttctt                           >  1:122262/1‑151 (MQ=255)                                                                                                                        cGATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATttcttc                          <  1:676451/151‑1 (MQ=255)                                                                                                                         gATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATttcttc                          >  1:258147/1‑150 (MQ=255)                                                                                                                         gATATTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATttctt                           <  1:759248/149‑1 (MQ=255)                                                                                                                          atatTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCAt                        >  2:23001/1‑151 (MQ=255)                                                                                                                           tatTTTAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATc                       >  2:50190/1‑151 (MQ=255)                                                                                                                             ttttAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATcc                      >  2:874289/1‑150 (MQ=255)                                                                                                                              tttAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCcg                     <  2:369166/150‑1 (MQ=255)                                                                                                                                tAAGCACAGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCgcgc                  >  1:429/1‑151 (MQ=255)                                                                                                                                    cacaGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCg              <  1:391404/151‑1 (MQ=255)                                                                                                                                    cacaGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCg              <  1:932684/151‑1 (MQ=255)                                                                                                                                      caGTAGTGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGa            <  2:64866/151‑1 (MQ=255)                                                                                                                                          attGGGTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTg        >  2:635394/3‑151 (MQ=255)                                                                                                                                               gTGCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGAAt   <  2:677673/151‑1 (MQ=255)                                                                                                                                                 gCATGATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCAtt  <  2:23253/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  GCAACAATTTTGAAGTTTGATCTGGAGATGCTTAAAAAACATCGCAGAGCAATCCAGTTTATTGCCGTGCTGCTGTTTATCGTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATT  >  NC_000913/3657013‑3657306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑