Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,659,521	T→G	L97* (TTA→TGA)	mdtE →	anaerobic multidrug efflux transporter, ArcA‑regulated

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,659,521	0	T	G	100.0%	246.0 / NA	66	L97* (TTA→TGA)	mdtE	anaerobic multidrug efflux transporter, ArcA‑regulated
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (36/30);  total (36/30)

GTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTGAAGACCAACTACGTTAGCCGTCAGGATTACGACACCGCGCGCACCCAGT  >  NC_000913/3659375‑3659667                                                                                                                                                   |                                                                                                                                                   gtTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACa                                                                                                                                                 <  2:491588/150‑1 (MQ=255)   tGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAgg                                                                                                                                               <  1:418758/150‑1 (MQ=255)      gCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGa                                                                                                                                           <  1:719585/151‑1 (MQ=255)         aaTTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCt                                                                                                                                        <  2:922390/151‑1 (MQ=255)                 ggTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCg                                                                                                                                >  2:757813/1‑151 (MQ=255)                          gTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCt                                                                                                                       <  2:806663/151‑1 (MQ=255)                            tCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCg                                                                                                                     >  1:634101/1‑151 (MQ=255)                                tATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTg                                                                                                                  <  1:910434/150‑1 (MQ=255)                                 aTGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTgg                                                                                                                 <  2:130035/150‑1 (MQ=255)                                   gAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCg                                                                                                               <  1:42473/150‑1 (MQ=255)                                     aGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGaa                                                                                                             >  2:77703/1‑150 (MQ=255)                                          ccGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAgcgc                                                                                                        >  1:243994/1‑150 (MQ=255)                                          ccGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGct                                                                                                       >  2:568881/1‑151 (MQ=255)                                           cGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGctc                                                                                                      <  2:773921/151‑1 (MQ=255)                                            gagaTACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGctct                                                                                                     >  2:908794/1‑151 (MQ=255)                                            gagaTACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGctct                                                                                                     >  1:303888/1‑151 (MQ=255)                                                 aCGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTAcc                                                                                                >  1:447010/1‑151 (MQ=255)                                                  cGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCg                                                                                               >  1:751512/1‑151 (MQ=255)                                                     ccccAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGcc                                                                                             >  2:193875/1‑150 (MQ=255)                                                     ccccAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCa                                                                                            <  2:597658/151‑1 (MQ=255)                                                           gTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATg                                                                                      >  2:216983/1‑151 (MQ=255)                                                             gggCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGcc                                                                                    <  1:544957/151‑1 (MQ=255)                                                                cGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGc                                                                                 >  1:163213/1‑151 (MQ=255)                                                                   tattatCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATc                                                                              >  1:299392/1‑151 (MQ=255)                                                                    attatCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAACAATGCCCGCATc                                                                              <  2:413551/150‑1 (MQ=255)                                                                      tatCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCAcc                                                                           >  1:276894/1‑151 (MQ=255)                                                                      tatCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCAcc                                                                           >  2:148984/1‑151 (MQ=255)                                                                         cATTAAACGCATCTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCtt                                                                         <  1:411973/150‑1 (MQ=255)                                                                         cATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCtt                                                                         <  2:243571/150‑1 (MQ=255)                                                                          aTTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTa                                                                       <  2:728721/151‑1 (MQ=255)                                                                            tAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAAc                                                                     >  1:213126/1‑151 (MQ=255)                                                                              aaCGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAAcc                                                                    >  2:700298/1‑150 (MQ=255)                                                                                 gCATCTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCCCCTTTAACCGCCa                                                                <  2:451850/151‑1 (MQ=255)                                                                                 gCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGcc                                                                 >  2:648918/1‑150 (MQ=255)                                                                                  cAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGcc                                                                 >  2:442350/1‑149 (MQ=255)                                                                                   acttttATCGAAGGCGATAAAGTGAACAAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTGGACAGGCCGAGCTAAACCCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCa                                                                <  1:4485/146‑1 (MQ=255)                                                                                    aCTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGc                                                             <  2:329653/151‑1 (MQ=255)                                                                                      tttATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGTTCCTGCCCCTTGACAGGCCGAGCTAAACCCCCCCAAAGGCTCGCTGGCGAAAGCGCTCTCTCCCGCCAGCAATGCCCGCATCCCCTTTAACCGCCAgg                                                              <  2:152939/148‑1 (MQ=255)                                                                                      tttATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCAt                                                           >  2:609488/1‑151 (MQ=255)                                                                                        tATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCg                                                         >  1:461621/1‑151 (MQ=255)                                                                                           cGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGtt                                                       <  1:272341/150‑1 (MQ=255)                                                                                            gAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGc                                                     >  1:543600/1‑151 (MQ=255)                                                                                             aaGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTg                                                      <  1:556063/149‑1 (MQ=255)                                                                                              aGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTg                                                   >  2:455821/1‑151 (MQ=255)                                                                                              aGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTg                                                   <  2:818343/151‑1 (MQ=255)                                                                                                 cGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTGAAg                                                <  2:343724/151‑1 (MQ=255)                                                                                                  gATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTGa                                                  >  1:210391/1‑148 (MQ=255)                                                                                                         tGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTGAAGACCAACt                                         >  1:207768/1‑150 (MQ=255)                                                                                                          gAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTGAAGACCAACTAc                                       >  1:340922/1‑151 (MQ=255)                                                                                                              cAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTGAAGACCAACTACGtt                                    >  1:523152/1‑150 (MQ=255)                                                                                                               aGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTGAAGACCAACTACGtt                                    >  2:464365/1‑149 (MQ=255)                                                                                                               aGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTGAAGACCAACTACGtt                                    >  2:649461/1‑149 (MQ=255)                                                                                                                 ggCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTGAAGACCAACTACGTTAGcc                                <  2:413441/151‑1 (MQ=255)                                                                                                                  gCGATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTGAAGACCAACTACGTTAGCCg                               >  1:60717/1‑151 (MQ=255)                                                                                                                    gATTCGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGTATCGTTGCTGAAGACCAACTACGTTAGCCGTc                             >  1:421862/1‑151 (MQ=255)                                                                                                                        cGCTGTATCAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTGAAGACCAACTACGTTAGCCGTCAGGa                         <  2:642833/151‑1 (MQ=255)                                                                                                                         gCTGTATCAGATTGATCCTGCCCCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTGAAGACCAACTACGTTAGCCGTCAGGAt                        <  2:790422/151‑1 (MQ=255)                                                                                                                                cAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTGAAGACCAACTACGTTAGCCGTCAGGATTAc                     >  1:296235/1‑147 (MQ=255)                                                                                                                                cAGATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTGAAGACCAACTACGTTAGCCGTCAGGATTACGac                  <  2:127808/150‑1 (MQ=255)                                                                                                                                  gATTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTGAAGACCAACTACGTTAGCCGTCAGGATTACGACAcc               >  1:823813/1‑151 (MQ=255)                                                                                                                                   aTTGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTGAAGACCAACTACGTTAGCCGTCAGGATTACGACAcc               >  1:131730/1‑150 (MQ=255)                                                                                                                                     tGATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTGAAGACCAACTACGTTAGCCGTCAGGATTACGACACcgcg            >  1:511225/1‑151 (MQ=255)                                                                                                                                      gATCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTGAAGACCAACTACGTTAGCCGTCAGGATTACGACACcgcg            <  2:43525/150‑1 (MQ=255)                                                                                                                                        tCCTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTGAAGACCAACTACGTTAGCCGTCAGGATTACGACACCgcgcgc         >  2:61067/1‑151 (MQ=255)                                                                                                                                         ccTGCACCTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTGAAGACCAACTACGTTAGCCGTCAGGATTACGACACCgcgcgc         <  2:297743/150‑1 (MQ=255)                                                                                                                                               ccTTGACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTGAAGACCAACTACGTTAGCCGTCAGGATTACGACACCGCGCGCACCCAGt  <  2:548685/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   GTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTGAAGACCAACTACGTTAGCCGTCAGGATTACGACACCGCGCGCACCCAGT  >  NC_000913/3659375‑3659667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑