Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,681,475	T→G	I156L (ATC→CTC)	yhjJ ←	putative periplasmic M16 family chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,681,475	0	T	G	100.0%	184.2 / NA	53	I156L (ATC→CTC)	yhjJ	putative periplasmic M16 family chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (23/30);  total (23/30)

TCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGATGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTCAGATTAAACAGCGTGGTGTCATAAGAGACAATTACCGGCGGCATCGGGCGTTT  >  NC_000913/3681333‑3681619                                                                                                                                               |                                                                                                                                                 tCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCt                                                                                                                                           <  2:243903/150‑1 (MQ=255)   cGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCCGCGCGGGGTTGAGGGGTTTTgg                                                                                                                                         >  2:575465/1‑150 (MQ=255)   cGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTgg                                                                                                                                         >  2:832715/1‑150 (MQ=255)            gggTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGCTGCCACCATGTCCCGACTTTGCAGCGCGTGGGTGAGGGGTTCTTGTGTGATGGTc                                                                                                                               >  1:34709/1‑151 (MQ=255)             ggTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCa                                                                                                                              <  2:779052/151‑1 (MQ=255)              gttgttTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGGTGAGGGGTTCTGGTGTGATGGTCa                                                                                                                              >  1:519247/1‑150 (MQ=255)                 gttTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAAttt                                                                                                                          <  2:890045/151‑1 (MQ=255)                  tttCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCCGCGCGTGGTTGAGGGTTTCTTGTGTGATGGGCAAttt                                                                                                                          >  1:585694/1‑150 (MQ=255)                    tCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGcc                                                                                                                       >  1:818213/1‑151 (MQ=255)                     cAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGcc                                                                                                                       >  2:810542/1‑150 (MQ=255)                      aGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAg                                                                                                                     <  1:578246/151‑1 (MQ=255)                                  ggATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCa                                                                                                         <  1:174730/151‑1 (MQ=255)                                      cGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGAt                                                                                                      <  1:367079/150‑1 (MQ=255)                                       gTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGGCACCATGTCCTGCCTTTGCAGCGCGTGGTTGGGGGTTTCTGGCGTGATGGTCCATTTGCCCGTGGTATTTGCCAGTTa                                                                                                     >  1:185463/1‑150 (MQ=255)                                               aCAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAgcgc                                                                                            >  2:132395/1‑151 (MQ=255)                                                 aaGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGctt                                                                                          >  2:79783/1‑151 (MQ=255)                                                      tGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCttt                                                                                      <  1:380213/150‑1 (MQ=255)                                                       gACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTc                                                                                     >  2:536101/1‑150 (MQ=255)                                                        aCCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTc                                                                                     >  2:630508/1‑149 (MQ=255)                                                         cccTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGc                                                                                  <  2:325487/151‑1 (MQ=255)                                                              tCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCCGATAAGAGAGCGCTTCTTTCAGCAAATc                                                                             >  1:614268/1‑151 (MQ=255)                                                                aGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGt                                                                           >  1:708077/1‑151 (MQ=255)                                                                aGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGt                                                                           <  1:567137/151‑1 (MQ=255)                                                                       aGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGtt                                                                    <  2:896433/151‑1 (MQ=255)                                                                                   ccTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGGTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAAc                                                         >  1:373600/1‑150 (MQ=255)                                                                                      ctTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTc                                                       >  2:839821/1‑149 (MQ=255)                                                                                          aGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTCAGAtt                                                  <  1:594373/150‑1 (MQ=255)                                                                                          aGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTCAGATTa                                                 <  2:45153/151‑1 (MQ=255)                                                                                          aGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTCAGATTa                                                 <  2:346372/151‑1 (MQ=255)                                                                                            tATCGGCAGGCCAGGGTGCCACCATGTCCTGACTTTGCAGCGCGTGGGTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTCAGATTaa                                                >  1:702969/1‑150 (MQ=255)                                                                                             aTCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTCAGATTaaa                                               <  2:480074/150‑1 (MQ=255)                                                                                               cGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTCAGATTAAACa                                             <  1:390340/150‑1 (MQ=255)                                                                                                 gCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTCAGATTAAACAGCg                                          <  2:26231/151‑1 (MQ=255)                                                                                                       cAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTCAGATTAAACAGCGTGgtgt                                    <  2:645783/151‑1 (MQ=255)                                                                                                         ggTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTCAGATTAAACAGCGTGGTGTCa                                  <  2:288581/151‑1 (MQ=255)                                                                                                           ttGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTCAGATTAAACAGCGTGGTGTCAt                                 >  2:899976/1‑150 (MQ=255)                                                                                                           ttGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTCAGATTAAACAGCGTGGTGTCAt                                 >  2:244167/1‑150 (MQ=255)                                                                                                              ccaccaTGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTCAGATTAAACAGCGTGGTGTCATAaga                             <  1:524034/151‑1 (MQ=255)                                                                                                                  caTGTCCTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTCAGATTAAACAGCGTGGTGTCATAAGAGACa                         <  1:561941/151‑1 (MQ=255)                                                                                                                       ccTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTGCGGTTATTGGGCAAACTCAGATTAAACAGCGTGGTGTCATAAGAGACAATTAc                    <  1:832695/151‑1 (MQ=255)                                                                                                                       ccTGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTCAGATTAAACAGCGTGGTGTCATAAGAGACAATTAc                    <  1:414402/151‑1 (MQ=255)                                                                                                                         tGACTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTCAGATTAAACAGCGTGGTGTCATAAGAGACAATTACcg                  >  1:63269/1‑151 (MQ=255)                                                                                                                           aCTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTCAGATTAAACAGCGTGGTGTCATAAGAGACAATTACcggc                <  2:291643/151‑1 (MQ=255)                                                                                                                           aCTTTGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTCAGATTAAACAGCGTGGTGTCATAAGAGACAATTACcggc                <  2:595332/151‑1 (MQ=255)                                                                                                                              ttGCAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTCAGATTAAACAGCGTGGTGTCATAAGAGACAATTACcggcgg              <  1:716733/150‑1 (MQ=255)                                                                                                                                 cAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTCAGATTAAACAGCGTGGTGTCATAAGAGACAATTACCGGCGGCATc          <  1:442265/151‑1 (MQ=255)                                                                                                                                 cAGCGCGTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTCAGATTAAACAGCGTGGTGTCATAAGAGACAATTACCGGCGGCATc          >  1:355953/1‑151 (MQ=255)                                                                                                                                    cgcgTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTCAGATTAAACAGCGTGGTGTCATAAGAGACAATTACCGGCGGCATCggg       <  1:124615/151‑1 (MQ=255)                                                                                                                                     gcgTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTCAGATTAAACAGCGTGGTGTCATAAGAGACAATTACCGGCGGCATCGGGc      >  2:188487/1‑151 (MQ=255)                                                                                                                                       gTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTCAGATTAAACAGCGTGGTGTCATAAGAGACAATTACCGGCGGCATCGGGCGt    <  1:536084/151‑1 (MQ=255)                                                                                                                                       gTGGTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTCAGATTAAACAGCGTGGTGTCATAAGAGACAATTACCGGCGGCATCGGGCGt    <  2:229831/151‑1 (MQ=255)                                                                                                                                         ggTTGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTCAGATTAAACAGCGTGGTGTCATAAGAGACAATTACCGGCGGCATCGGGCGttt  <  1:479937/151‑1 (MQ=255)                                                                                                                                           ttGAGGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTCAGATTAAACAGCGTGGTGTCATAAGAGACAATTACCGGCGGCATCGGGCGttt  >  2:622264/1‑149 (MQ=255)                                                                                                                                               |                                                                                                                                                 TCCGCTTCAACGGGTTGTTTCAGCGGATCGGCAGGATCGTGACCTAACAAGGTTGACCCTTTCAGACGATAGCGCCACCAGCCCTCTTTAGTATCGGCAGGCCAGGTTGCCACCATGTCCTGACTTTGCAGCGCGTGGTTGATGGTTTCTGGTGTGATGGTCAATTTGCCAGTGGCATTTGCCAGATAAGAGAGCGCTTCTTTCAGCAAATCGTTACGGTTATTGGGCAAACTCAGATTAAACAGCGTGGTGTCATAAGAGACAATTACCGGCGGCATCGGGCGTTT  >  NC_000913/3681333‑3681619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑