Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,791,001	A→C	V115V (GTT→GTG)	tdh ←	L‑threonine 3‑dehydrogenase, NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,791,001	0	A	C	100.0%	141.6 / NA	40	V115V (GTT→GTG)	tdh	L‑threonine 3‑dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (19/21);  total (19/21)

GCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACC  >  NC_000913/3790854‑3791147                                                                                                                                                    |                                                                                                                                                   gCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCac                                                                                                                                                  >  2:79487/1‑150 (MQ=255)   ccaccaGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACAcc                                                                                                                                               <  2:24011/151‑1 (MQ=255)      ccaGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAAc                                                                                                                                            >  1:117728/1‑151 (MQ=255)      ccaGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAAc                                                                                                                                            <  1:108800/151‑1 (MQ=255)           tCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCt                                                                                                                                        >  2:647116/1‑150 (MQ=255)               aCGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCgt                                                                                                                                   <  1:252232/151‑1 (MQ=255)                         ggTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGcac                                                                                                                         <  2:915125/151‑1 (MQ=255)                         ggTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGcac                                                                                                                         <  2:212660/151‑1 (MQ=255)                          gTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGcaca                                                                                                                        <  1:931885/151‑1 (MQ=255)                                        aCCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGacca                                                                                                          <  2:598981/151‑1 (MQ=255)                                        aCCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGacc                                                                                                           >  1:216215/1‑150 (MQ=255)                                         ccGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGaccac                                                                                                         <  2:168780/151‑1 (MQ=255)                                                   cAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGtt                                                                                               >  2:626527/1‑151 (MQ=255)                                                        aTTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGc                                                                                          <  2:107218/151‑1 (MQ=255)                                                          tGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCa                                                                                         <  1:243391/150‑1 (MQ=255)                                                            cGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATg                                                                                      <  2:459003/151‑1 (MQ=255)                                                             ggCTAAGTCATCGGAAATATTGTCGCGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCACAAATTGGTACGACCACCACGACAGTTGCCGCAATGa                                                                                     <  1:675791/151‑1 (MQ=255)                                                                    tCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAATCAGGCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCCCGAATGTGTACGATCACCACGACAGTTGCGGCAAGGACCACAg                                                                               <  1:671013/150‑1 (MQ=255)                                                                        cGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGat                                                                          >  1:739703/1‑151 (MQ=255)                                                                         ggAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGat                                                                          >  2:661205/1‑150 (MQ=255)                                                                           aaaTATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATg                                                                       >  2:660829/1‑151 (MQ=255)                                                                                 tGTCGGGGATTTTGAAAGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCtt                                                                  <  1:768162/150‑1 (MQ=255)                                                                                     ggggATTTTGAAGGCGTTGAATGCCGGGATCGCCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGc                                                               <  1:400777/149‑1 (MQ=255)                                                                                       ggATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGcc                                                              >  2:260833/1‑148 (MQ=255)                                                                                        gATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGaa                                                          <  1:413679/151‑1 (MQ=255)                                                                                         aTTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGaa                                                          >  2:370085/1‑150 (MQ=255)                                                                                              gAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGa                                                    <  1:612186/151‑1 (MQ=255)                                                                                                    gTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCg                                              >  2:702788/1‑151 (MQ=255)                                                                                                        aaTGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCt                                          <  2:347434/151‑1 (MQ=255)                                                                                                         aTGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCtt                                         >  1:532624/1‑151 (MQ=255)                                                                                                            ccGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGaa                                      <  2:350634/151‑1 (MQ=255)                                                                                                                 aTCACCAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCtt                                 >  2:909934/1‑151 (MQ=255)                                                                                                                     ccAGATATTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCAc                             >  2:3243/1‑151 (MQ=255)                                                                                                                          tatTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCt                        >  2:324307/1‑151 (MQ=255)                                                                                                                           atTCGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCt                        <  2:698117/150‑1 (MQ=255)                                                                                                                              cGGCAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGAcc                    >  2:518355/1‑151 (MQ=255)                                                                                                                                 cAAAGCAGCCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAAt                 >  2:765493/1‑151 (MQ=255)                                                                                                                                     gcagcCCGGGCGATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAAtacct             <  1:510859/151‑1 (MQ=255)                                                                                                                                                gATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCAcc  >  1:482590/1‑151 (MQ=255)                                                                                                                                                gATTCACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCAcc  >  1:539382/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACC  >  NC_000913/3790854‑3791147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑