Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,814,738	A→C	K82T (AAA→ACA)	slmA →	nucleoid occlusion factor, anti‑FtsZ division inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,814,738	0	A	C	100.0%	198.3 / NA	52	K82T (AAA→ACA)	slmA	nucleoid occlusion factor, anti‑FtsZ division inhibitor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (26/26);  total (26/26)

GGCAAAACTGGCCGCCTCTGTCGGCGTTTCCGAAGCGGCACTGTATCGCCACTTCCCCAGTAAGACCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTTCGAGC  >  NC_000913/3814595‑3814884                                                                                                                                                |                                                                                                                                                   ggCAAACCTGGCCGCCTCTGTCGGCGTTTCCGAAGCGGCACTGTATCGCCACTTCCCCAGTAAGACCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGAcacc                                                                                                                                             <  1:135410/151‑1 (MQ=255)     aaaCTGGCCGCCTCTGTCGGCGTTTCCGAAGCGGCACTGTATCGCCACTTCCCCAGTAAGACCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGAcaccac                                                                                                                                           >  2:857925/1‑149 (MQ=255)     aaaCTGGCCGCCTCTGTCGGCGTTTCCGAAGCGGCACTGTATCGCCACTTCCCCAGTAAGACCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGAcaccac                                                                                                                                           >  2:761133/1‑149 (MQ=255)     aaaCTGGCCGCCTCTGTCGGCGTTTCCGAAGCGGCACTGTATCGCCACTTCCCCAGTAAGACCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAg                                                                                                                                         <  2:113299/151‑1 (MQ=255)       aCTGGCCGCCTCTGTCGGCGTTTCCGAAGCGGCACTGTATCGCCACTTCCCCAGTAAGACCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAgcg                                                                                                                                       <  1:806742/151‑1 (MQ=255)             cgccTCTGTCGGCGTTTCCGAAGCGGCACTGTATCGCCACTTCCCCAGTAAGACCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTg                                                                                                                                 <  1:261733/151‑1 (MQ=255)               ccTCTGTCGGCGTTTCCGAAGCGGCACTGTATCGCCACTTCCCCAGTAAGACCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCg                                                                                                                               <  1:430348/151‑1 (MQ=255)                       ggCGTTTCCGAAGCGGCACTGTATCGCCACTTCCCCAGTAAGACCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAGCCTGATTCTGACAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATtg                                                                                                                       <  1:396197/151‑1 (MQ=255)                       ggCGTTTCCGAAGCGGCACTGTATCGCCACTTCCCCAGTAAGACCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATtg                                                                                                                       <  1:754880/151‑1 (MQ=255)                           tttCCGAAGCGGCACTGTATCGCCACTTCCCCAGTAAGACCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGtt                                                                                                                   >  2:16772/1‑151 (MQ=255)                            ttCCGAAGCGGCACTGTATCGCCACTTCCCCAGTAAGACCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGtt                                                                                                                   <  2:69485/150‑1 (MQ=255)                               cGAAGCGGCACTGTATCGCCACTTCCCCAGTAAGACCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTtgctg                                                                                                               <  2:60864/151‑1 (MQ=255)                                gAAGCGGCACTGTATCGCCACTTCCCCAGTAAGACCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTtgctgc                                                                                                              >  2:875319/1‑151 (MQ=255)                                 aaGCGGCACTGTATCGCCACTTCCCCAGTAAGACCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTgctgct                                                                                                             >  1:436737/1‑151 (MQ=255)                                  aGCGGCACTGTATCGCCACTTCCCCAGTAAGACCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCtt                                                                                                            <  1:446168/151‑1 (MQ=255)                                    cGGCACTGTATCGCCACTTCCCCAGTAAGACCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTtct                                                                                                          >  2:494828/1‑151 (MQ=255)                                    cGGCACTGTATCGCCACTTCCCCAGTAAGACCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTtct                                                                                                          <  2:364874/151‑1 (MQ=255)                                    cGGCACTGTATCGCCACTTCCCCAGTAAGACCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTtct                                                                                                          >  1:226891/1‑151 (MQ=255)                                       cACTGTATCGCCACTTCCCCAGTAAGACCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCgg                                                                                                       <  2:191390/151‑1 (MQ=255)                                        aCTGTATCGCCACTTCCCCAGTAAGACCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGt                                                                                                      >  1:667/1‑151 (MQ=255)                                             aTCGCCACTTCCCCAGTAAGACCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTgg                                                                                                 >  2:789323/1‑151 (MQ=255)                                                     ttCCCCAGTAAGACCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGt                                                                                          >  1:488171/1‑150 (MQ=255)                                                      tCCCCAGTAAGACCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTa                                                                                         >  1:737390/1‑150 (MQ=255)                                                        cccAGTAAGACCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATc                                                                                      <  1:21893/151‑1 (MQ=255)                                                            gTAAGACCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTgg                                                                                  >  2:253309/1‑151 (MQ=255)                                                                 aCCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGa                                                                             <  1:106779/151‑1 (MQ=255)                                                                  cccGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGAc                                                                            >  2:166413/1‑151 (MQ=255)                                                                    cGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGAccc                                                                          <  1:803798/151‑1 (MQ=255)                                                                    cGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGAccc                                                                          <  2:628185/151‑1 (MQ=255)                                                                     gCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCg                                                                         >  2:556760/1‑151 (MQ=255)                                                                       aTGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCGc                                                                        >  1:444664/1‑150 (MQ=255)                                                                            cGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCGCATCCTc                                                                  >  1:119158/1‑151 (MQ=255)                                                                                 gCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCGCATCCTCACTgg                                                             >  1:51487/1‑151 (MQ=255)                                                                                 gCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCGCATCCTCACTgg                                                             >  1:534198/1‑151 (MQ=255)                                                                                     gATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCGCATCCTCACTGGTc                                                           <  1:108657/149‑1 (MQ=255)                                                                                     gATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGCGAGCGTAATCCTGGCCTGACCCGCATCCTCACTGGTCAt                                                         >  2:155748/1‑151 (MQ=255)                                                                                               aTCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATg                                                <  2:737950/150‑1 (MQ=255)                                                                                               aTCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGt                                               >  2:597936/1‑151 (MQ=255)                                                                                                  gAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTg                                            >  1:834583/1‑151 (MQ=255)                                                                                                  gAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTg                                            >  2:596420/1‑151 (MQ=255)                                                                                                   aaGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGa                                           <  2:314069/151‑1 (MQ=255)                                                                                                      aTAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACa                                        <  1:901363/151‑1 (MQ=255)                                                                                                        agcgagATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCTTGGCTTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAg                                       <  1:430780/145‑1 (MQ=255)                                                                                                        aGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAg                                       <  1:926441/150‑1 (MQ=255)                                                                                                         gCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGa                                     <  2:391715/151‑1 (MQ=255)                                                                                                            tGATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCg                                  >  2:38055/1‑151 (MQ=255)                                                                                                             gATTACTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGc                                 <  2:394259/151‑1 (MQ=255)                                                                                                                  cTCGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTg                              <  1:606584/149‑1 (MQ=255)                                                                                                                    cGCATCAACCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAg                          <  1:199371/151‑1 (MQ=255)                                                                                                                           aCCTGATTCTGAAAGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCTTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCAt                   >  2:118967/1‑151 (MQ=255)                                                                                                                                       aaGATGAGACAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCCTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGtt       >  2:149847/1‑151 (MQ=255)                                                                                                                                            gagaCAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTTCGAGc  >  2:124194/1‑151 (MQ=255)                                                                                                                                                |                                                                                                                                                   GGCAAAACTGGCCGCCTCTGTCGGCGTTTCCGAAGCGGCACTGTATCGCCACTTCCCCAGTAAGACCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACTCGCATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTTCGAGC  >  NC_000913/3814595‑3814884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑