Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	216,474	A→C	F177C (TTC→TGC)	yaeF ←	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	216,474	0	A	C	100.0%	259.2 / NA	71	F177C (TTC→TGC)	yaeF	putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (19/52);  total (19/52)

CCAGTGTAAGTGGATGCCCGGCTTTGGCAAAGGCATCCGTGACAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAG  >  NC_000913/216331‑216620                                                                                                                                                |                                                                                                                                                   ccAGTGTAAGTGGATGCCCGGCTTTGGCAAAGGCATCCGTGACAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGAtt                                                                                                                                             <  2:18064/151‑1 (MQ=255) ccAGTGTAAGTGGATGCCCGGCTTTGGCAAAGGCATCCGTGACAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGAtt                                                                                                                                             <  2:885668/151‑1 (MQ=255)     tgtAAGTGGATGCCCGGCTTTGGCAAAGGCATCCGTGACAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGt                                                                                                                                         >  2:924997/1‑151 (MQ=255)       tAAGTGGATGCCCGGCTTTGGCAAAGGCATCCGTGACAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTg                                                                                                                                        >  2:717444/1‑150 (MQ=255)       tAAGTGGATGCCCGGCTTTGGCAAAGGCATCCGTGACAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTg                                                                                                                                        >  1:923702/1‑150 (MQ=255)          gTGGATGCCCGGCTTTGGCAAAGGCATCCGTGACAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGca                                                                                                                                    <  1:215167/151‑1 (MQ=255)           tGGATGCCCGGCTTTGGCAAAGGCATCCGTGACAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGcac                                                                                                                                   >  2:83442/1‑151 (MQ=255)             gATGCCCGGCTTTGGCAAAGGCATCCGTGACAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACAt                                                                                                                                 <  1:438101/151‑1 (MQ=255)                gCCCGGCTTTGGCAAAGGCATCCGTGACAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCt                                                                                                                               <  2:287098/150‑1 (MQ=255)                gCCCGGCTTTGGCAAAGGCATCCGTGACAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTg                                                                                                                              <  1:354545/151‑1 (MQ=255)                gCCCGGCTTTGGCAAAGGCATCCGTGACAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTg                                                                                                                              <  2:70699/151‑1 (MQ=255)                 cccGGCTTTGGCAAAGGCATCCGTGACAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTgg                                                                                                                             <  1:185843/151‑1 (MQ=255)                  ccGGCTTTGGCAAAGGCATCCGTGACAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGc                                                                                                                            <  2:155140/151‑1 (MQ=255)                  ccGGCTTTGGCAAAGGCATCCGTGACAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGc                                                                                                                            <  2:4749/151‑1 (MQ=255)                   cGGCTTTGGCAAAGGCATCCGTGACAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCg                                                                                                                           <  1:720521/151‑1 (MQ=255)                    ggCTTTGGCAAAGGCATCCGTGACAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGa                                                                                                                          <  2:851557/151‑1 (MQ=255)                          ggCAAAGGCATCCGTGACAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCAcc                                                                                                                    <  1:327056/151‑1 (MQ=255)                           gCAAAGGCATCCGTGACAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCAcc                                                                                                                    >  2:565847/1‑150 (MQ=255)                             aaaGGCATCCGTGACAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATa                                                                                                                 <  2:768506/151‑1 (MQ=255)                             aaaGGCATCCGTGACAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCGCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCAt                                                                                                                  <  2:908682/150‑1 (MQ=255)                              aaGGCATCCGTGACAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATa                                                                                                                 >  1:824300/1‑150 (MQ=255)                               aGGCATCCGTGACAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATaaa                                                                                                               <  2:831845/151‑1 (MQ=255)                                ggCATCCGTGACAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAg                                                                                                              <  2:14912/151‑1 (MQ=255)                                 gCATCCGTGACAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAgg                                                                                                             >  1:652692/1‑151 (MQ=255)                                     ccGTGACAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCGTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGGGAGCACATCTGGCGAGTCACCATAAAGGGaa                                                                                                          <  2:332486/150‑1 (MQ=255)                                           cAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTc                                                                                                   <  2:649824/151‑1 (MQ=255)                                           cAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTc                                                                                                   >  2:146526/1‑151 (MQ=255)                                            aaaTTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCg                                                                                                  <  2:490985/151‑1 (MQ=255)                                              aTTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGAc                                                                                                >  1:63839/1‑151 (MQ=255)                                                 ccGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTGAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACaa                                                                                              <  2:572741/150‑1 (MQ=255)                                                  cGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATg                                                                                            <  1:623734/151‑1 (MQ=255)                                                  cGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATg                                                                                            <  1:24169/151‑1 (MQ=255)                                                      cAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCcgc                                                                                        <  1:506722/151‑1 (MQ=255)                                                       aaaaCCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCcgcg                                                                                       <  2:922178/151‑1 (MQ=255)                                                          aCCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATa                                                                                    <  1:750894/151‑1 (MQ=255)                                                           ccACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAg                                                                                   <  2:307657/151‑1 (MQ=255)                                                                     ttGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCg                                                                          <  2:242394/150‑1 (MQ=255)                                                                      tGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGc                                                                         <  1:561691/150‑1 (MQ=255)                                                                      tGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCt                                                                        >  2:354104/1‑151 (MQ=255)                                                                       gTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCt                                                                        <  2:666888/150‑1 (MQ=255)                                                                       gTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCt                                                                        <  2:162969/150‑1 (MQ=255)                                                                       gTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTa                                                                       <  2:180473/151‑1 (MQ=255)                                                                           ccTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTAt                                                                      <  1:321075/148‑1 (MQ=255)                                                                           ccTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATc                                                                     <  1:740734/149‑1 (MQ=255)                                                                           ccTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCtt                                                                   <  1:867684/151‑1 (MQ=255)                                                                           ccTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCt                                                                    <  2:33118/150‑1 (MQ=255)                                                                             ttCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTg                                                                 >  1:804138/1‑151 (MQ=255)                                                                              tCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTg                                                                 <  1:161816/150‑1 (MQ=255)                                                                               cGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCCCCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCttt                                                                  <  1:716542/148‑1 (MQ=255)                                                                                 cccACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGAttt                                                             <  1:271740/151‑1 (MQ=255)                                                                                    acacTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTAt                                                          <  2:835240/151‑1 (MQ=255)                                                                                        tGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGc                                                      >  1:902214/1‑151 (MQ=255)                                                                                                 gcgcTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAAcgcg                                               <  2:342061/149‑1 (MQ=255)                                                                                                  cgcTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTg                                            <  1:315830/151‑1 (MQ=255)                                                                                                   gcTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGa                                           <  1:116061/151‑1 (MQ=255)                                                                                                        cGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATc                                       <  1:73370/150‑1 (MQ=255)                                                                                                          ccAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCtgt                                    <  1:661999/151‑1 (MQ=255)                                                                                                           cAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCtgtg                                   <  1:766815/151‑1 (MQ=255)                                                                                                              gCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCt                                >  2:852667/1‑151 (MQ=255)                                                                                                                  cTGACGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCttgtt                            >  2:907739/1‑151 (MQ=255)                                                                                                                     aCGCACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCg                         <  1:824577/151‑1 (MQ=255)                                                                                                                        cACTGTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCgggg                      >  1:528853/1‑151 (MQ=255)                                                                                                                            gTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTa                    >  1:639739/1‑149 (MQ=255)                                                                                                                            gTTGGCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTa                    <  2:227721/149‑1 (MQ=255)                                                                                                                                gCGAAAATCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATcc              <  2:445798/151‑1 (MQ=255)                                                                                                                                    aaaTCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGa          <  2:767111/151‑1 (MQ=255)                                                                                                                                     aaTCCTCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGAc         >  2:388663/1‑151 (MQ=255)                                                                                                                                          tCGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGt     >  2:757630/1‑150 (MQ=255)                                                                                                                                           cGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTaa   <  2:28385/151‑1 (MQ=255)                                                                                                                                           cGGAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTa    >  1:630369/1‑150 (MQ=255)                                                                                                                                            ggAGCACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAg  <  2:197619/151‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                   CCAGTGTAAGTGGATGCCCGGCTTTGGCAAAGGCATCCGTGACAAATTCCGAACAAAACCACGACTTTTTGTCTCCTTCGCCCACACTGCTTAACTGCGCTTTCGCCAGGCCGCTGACGCACTGTTGGCGAAAATCCTCGGAGAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAG  >  NC_000913/216331‑216620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑