Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,972,742	A→C	Q74P (CAG→CCG)	rffG →	dTDP‑glucose 4,6‑dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,972,742	0	A	C	100.0%	253.9 / NA	69	Q74P (CAG→CCG)	rffG	dTDP‑glucose 4,6‑dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (31/38);  total (31/38)

ACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATA  >  NC_000913/3972594‑3972888                                                                                                                                                     |                                                                                                                                                   acgaGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGc                                                                                                                                                  >  1:461963/1‑151 (MQ=255)  cgaGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGcc                                                                                                                                                 <  1:640761/151‑1 (MQ=255)  cgaGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGcc                                                                                                                                                 >  2:110860/1‑151 (MQ=255)   gaGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACACGTATTCACTGAGCATCCGCCa                                                                                                                                                <  1:172154/151‑1 (MQ=255)    aGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGcc                                                                                                                                                 >  2:634506/1‑149 (MQ=255)    aGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGcc                                                                                                                                                 >  1:713008/1‑149 (MQ=255)     gCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGa                                                                                                                                              <  1:593166/151‑1 (MQ=255)       gACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACt                                                                                                                                            >  2:576420/1‑151 (MQ=255)       gACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACt                                                                                                                                            >  2:311910/1‑151 (MQ=255)                   gTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATc                                                                                                                                <  1:772939/151‑1 (MQ=255)                        cGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGc                                                                                                                            >  1:384525/1‑150 (MQ=255)                        cGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCa                                                                                                                           <  1:629538/151‑1 (MQ=255)                         gATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAg                                                                                                                          <  2:233355/151‑1 (MQ=255)                            aaGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCg                                                                                                                       <  2:308426/151‑1 (MQ=255)                                 gACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAg                                                                                                                   <  2:67723/150‑1 (MQ=255)                                 gACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGc                                                                                                                  <  2:339869/151‑1 (MQ=255)                                   ccTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGcc                                                                                                                 >  1:103654/1‑150 (MQ=255)                                       cGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGtt                                                                                                            <  2:832114/151‑1 (MQ=255)                                          cGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGAc                                                                                                         <  2:176130/151‑1 (MQ=255)                                          cGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGAc                                                                                                         >  2:27649/1‑151 (MQ=255)                                              aaCCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGtt                                                                                                     <  1:651780/151‑1 (MQ=255)                                                  tGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTAt                                                                                                 >  1:536852/1‑151 (MQ=255)                                                    aTGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTg                                                                                               >  1:400606/1‑151 (MQ=255)                                                     tGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTg                                                                                               <  1:715665/150‑1 (MQ=255)                                                        cGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACgg                                                                                           >  2:432312/1‑151 (MQ=255)                                                         gCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGc                                                                                          >  2:445554/1‑151 (MQ=255)                                                          cTGGCACACATCGCGCAAAGCGCGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGTGAAGAACTGTCACGCGTATTCGCGGAGCATCCGCCAGACTGTGTCATGCTTCTGGCAGCCGAAAACCATGTTGGCCGTTCTAGTGAcaga                                                                                          <  1:685050/150‑4 (MQ=255)                                                            ggCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGccc                                                                                        <  1:326085/150‑1 (MQ=255)                                                             gCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCgg                                                                                      >  1:345874/1‑151 (MQ=255)                                                                 cGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGc                                                                                  <  1:91198/151‑1 (MQ=255)                                                                 cGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGc                                                                                  >  2:686437/1‑151 (MQ=255)                                                                     cgcgCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGttt                                                                              <  2:677365/151‑1 (MQ=255)                                                                      gcgcAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGttt                                                                              >  2:742600/1‑150 (MQ=255)                                                                          aaaGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTAtgg                                                                          >  2:489128/1‑148 (MQ=255)                                                                           aaGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGaa                                                                        <  2:110872/151‑1 (MQ=255)                                                                             gcgagcgCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAAc                                                                      >  1:749023/1‑151 (MQ=255)                                                                                agcgCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCa                                                                    >  1:78605/1‑150 (MQ=255)                                                                                   gcTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACAt                                                                <  1:145067/151‑1 (MQ=255)                                                                                    cTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACAtt                                                               >  1:331155/1‑151 (MQ=255)                                                                                      ttGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACAtt                                                               <  1:450752/149‑1 (MQ=255)                                                                                        gCCTTTGAGAAAGTTGATATCTGCGATC‑GGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCgg                                                          <  1:280645/151‑1 (MQ=255)                                                                                              gagaAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACtt                                                     <  2:551690/151‑1 (MQ=255)                                                                                                gaAAGTTGATATCAGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTtat                                                   <  2:228650/151‑1 (MQ=255)                                                                                                 aaaGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTtata                                                  <  2:321378/151‑1 (MQ=255)                                                                                                      tGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACAtt                                              <  2:307296/150‑1 (MQ=255)                                                                                                         tatCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCt                                           <  1:379021/150‑1 (MQ=255)                                                                                                          atCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTg                                         >  1:183538/1‑151 (MQ=255)                                                                                                              gCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTg                                         <  2:607366/147‑1 (MQ=255)                                                                                                              gCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGaa                                       <  2:359094/149‑1 (MQ=255)                                                                                                                gATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAgcgg                                   >  1:734159/1‑151 (MQ=255)                                                                                                                 aTCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAgcggc                                  <  1:576402/151‑1 (MQ=255)                                                                                                                 aTCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAgcggc                                  <  1:515666/151‑1 (MQ=255)                                                                                                                    gggCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGcgcg                               >  1:571588/1‑151 (MQ=255)                                                                                                                    gggCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGcgcg                               <  1:349353/151‑1 (MQ=255)                                                                                                                        aGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCt                           <  2:64970/151‑1 (MQ=255)                                                                                                                        aGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCt                           >  2:208027/1‑151 (MQ=255)                                                                                                                          aaCTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCtt                          >  2:542423/1‑150 (MQ=255)                                                                                                                           aCTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTAc                        >  2:25478/1‑151 (MQ=255)                                                                                                                              ggCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTgg                     >  1:878711/1‑151 (MQ=255)                                                                                                                              ggCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTg                      <  2:539218/150‑1 (MQ=255)                                                                                                                              ggCACGCGTATTCACTAAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTgg                     >  2:396097/1‑151 (MQ=255)                                                                                                                                       aTTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTg            <  1:604757/151‑1 (MQ=255)                                                                                                                                         tCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGa           <  1:531807/150‑1 (MQ=255)                                                                                                                                         tCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGAAGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGa           <  2:713028/150‑1 (MQ=255)                                                                                                                                             tGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGa       >  2:62346/1‑150 (MQ=255)                                                                                                                                             tGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGa       >  2:223412/1‑150 (MQ=255)                                                                                                                                             tGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGa       >  2:769105/1‑150 (MQ=255)                                                                                                                                                gCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGa       <  1:379050/147‑1 (MQ=255)                                                                                                                                                 cATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATa  <  2:155004/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   ACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATA  >  NC_000913/3972594‑3972888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑