Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,972,815	T→G	I98M (ATT→ATG)	rffG →	dTDP‑glucose 4,6‑dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,972,815	0	T	G	100.0%	302.3 / NA	78	I98M (ATT→ATG)	rffG	dTDP‑glucose 4,6‑dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (35/43);  total (35/43)

AAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTATATGGTGACCTGCACTCGACGGATGATTT  >  NC_000913/3972668‑3972961                                                                                                                                                    |                                                                                                                                                   aaGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGaa                                                                                                                                                 <  2:110872/151‑1 (MQ=255)   gcgagcgCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAAc                                                                                                                                               >  1:749023/1‑151 (MQ=255)      agcgCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCa                                                                                                                                             >  1:78605/1‑150 (MQ=255)         gcTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACAt                                                                                                                                         <  1:145067/151‑1 (MQ=255)          cTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACAtt                                                                                                                                        >  1:331155/1‑151 (MQ=255)            ttGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACAtt                                                                                                                                        <  1:450752/149‑1 (MQ=255)              gCCTTTGAGAAAGTTGATATCTGCGATC‑GGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCgg                                                                                                                                   <  1:280645/151‑1 (MQ=255)                    gagaAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACtt                                                                                                                              <  2:551690/151‑1 (MQ=255)                      gaAAGTTGATATCAGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTtat                                                                                                                            <  2:228650/151‑1 (MQ=255)                       aaaGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTtata                                                                                                                           <  2:321378/151‑1 (MQ=255)                            tGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACAtt                                                                                                                       <  2:307296/150‑1 (MQ=255)                               tatCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCt                                                                                                                    <  1:379021/150‑1 (MQ=255)                                atCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTg                                                                                                                  >  1:183538/1‑151 (MQ=255)                                    gCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTg                                                                                                                  <  2:607366/147‑1 (MQ=255)                                    gCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGaa                                                                                                                <  2:359094/149‑1 (MQ=255)                                      gATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAgcgg                                                                                                            >  1:734159/1‑151 (MQ=255)                                       aTCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAgcggc                                                                                                           <  1:515666/151‑1 (MQ=255)                                       aTCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAgcggc                                                                                                           <  1:576402/151‑1 (MQ=255)                                          gggCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGcgcg                                                                                                        >  1:571588/1‑151 (MQ=255)                                          gggCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGcgcg                                                                                                        <  1:349353/151‑1 (MQ=255)                                              aGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCt                                                                                                    >  2:208027/1‑151 (MQ=255)                                              aGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCt                                                                                                    <  2:64970/151‑1 (MQ=255)                                                aaCTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCtt                                                                                                   >  2:542423/1‑150 (MQ=255)                                                 aCTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTAc                                                                                                 >  2:25478/1‑151 (MQ=255)                                                    ggCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTgg                                                                                              >  1:878711/1‑151 (MQ=255)                                                    ggCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTg                                                                                               <  2:539218/150‑1 (MQ=255)                                                    ggCACGCGTATTCACTAAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTgg                                                                                              >  2:396097/1‑151 (MQ=255)                                                             aTTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTg                                                                                     <  1:604757/151‑1 (MQ=255)                                                               tCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGa                                                                                    <  1:531807/150‑1 (MQ=255)                                                               tCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGAAGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGa                                                                                    <  2:713028/150‑1 (MQ=255)                                                                   tGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGa                                                                                >  2:769105/1‑150 (MQ=255)                                                                   tGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGa                                                                                >  2:62346/1‑150 (MQ=255)                                                                   tGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGa                                                                                >  2:223412/1‑150 (MQ=255)                                                                      gCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGa                                                                                <  1:379050/147‑1 (MQ=255)                                                                       cATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATa                                                                           <  2:155004/151‑1 (MQ=255)                                                                            gCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATaaaaaa                                                                      <  2:457369/151‑1 (MQ=255)                                                                            gCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATaaaaa                                                                       >  2:828871/1‑150 (MQ=255)                                                                               aGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATc                                                                    >  2:237770/1‑150 (MQ=255)                                                                               aGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCa                                                                   <  2:311624/151‑1 (MQ=255)                                                                                gACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATc                                                                    >  2:20952/1‑149 (MQ=255)                                                                                   tgtgTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGt                                                               >  2:814121/1‑151 (MQ=255)                                                                                      gtCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTcc                                                            >  2:826878/1‑151 (MQ=255)                                                                                        cATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCg                                                           <  2:461978/150‑1 (MQ=255)                                                                                        cATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGt                                                          <  2:321180/151‑1 (MQ=255)                                                                                           gCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGtttt                                                       <  1:910188/151‑1 (MQ=255)                                                                                             aTCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTtc                                                      >  2:886729/1‑150 (MQ=255)                                                                                                tGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTtcatc                                                   >  2:787136/1‑150 (MQ=255)                                                                                                 ggCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTcatcat                                                 <  2:17580/151‑1 (MQ=255)                                                                                                   cAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCatat                                               <  2:682646/151‑1 (MQ=255)                                                                                                     gCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATAtct                                             <  2:558928/151‑1 (MQ=255)                                                                                                       cGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTcc                                           >  1:647178/1‑151 (MQ=255)                                                                                                          aaGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACt                                        >  2:46675/1‑151 (MQ=255)                                                                                                            gCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGa                                      <  2:371546/151‑1 (MQ=255)                                                                                                                    gACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGtat                              <  1:445539/151‑1 (MQ=255)                                                                                                                    gACCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGta                               <  2:682999/150‑1 (MQ=255)                                                                                                                     aCCGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGtata                             <  2:722319/151‑1 (MQ=255)                                                                                                                      ccGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTatat                            <  1:634486/151‑1 (MQ=255)                                                                                                                       cGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTATATg                           >  2:435644/1‑151 (MQ=255)                                                                                                                       cGTTCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTATATg                           >  2:695050/1‑151 (MQ=255)                                                                                                                          tCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTATATGGt                         >  2:440655/1‑150 (MQ=255)                                                                                                                          tCTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTATATGGTg                        >  2:76399/1‑151 (MQ=255)                                                                                                                           cTATTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTATATGGTg                        >  1:652447/1‑150 (MQ=255)                                                                                                                             aTTGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTATATGGTGAc                      <  1:114377/150‑1 (MQ=255)                                                                                                                               tGACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTATATGGTGACCTg                   >  2:826034/1‑151 (MQ=255)                                                                                                                                gACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTATATGGTGACCTg                   <  2:691376/150‑1 (MQ=255)                                                                                                                                gACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTATATGGTGACCTGc                  <  1:478089/151‑1 (MQ=255)                                                                                                                                gACGGCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTATATGGTGACCTGc                  >  2:70934/1‑151 (MQ=255)                                                                                                                                   ggCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTATATGGTGACCTGCACt               <  2:760492/151‑1 (MQ=255)                                                                                                                                    gtacGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGTTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTATATGGTGACCTGCtctc              <  1:27648/148‑1 (MQ=255)                                                                                                                                    gCCCGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTATATGGTGACCTGCACTc              >  1:271173/1‑151 (MQ=255)                                                                                                                                      ccGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTATATGGTGACCTGCACTCGa            <  2:76935/151‑1 (MQ=255)                                                                                                                                       cGGCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTATATGGTGACCTGCACTCGAc           >  2:183882/1‑151 (MQ=255)                                                                                                                                        ggCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTATATGGTGACCTGCACTCGACg          <  1:765579/151‑1 (MQ=255)                                                                                                                                         gCAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTATATGGTGACCTGCACTCGACgg         >  2:673557/1‑151 (MQ=255)                                                                                                                                          cAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTATATGGTGACCTGCACTCGACgg         >  2:483126/1‑150 (MQ=255)                                                                                                                                          cAGCGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTATATGGTGACCTGCACTCGACGga        <  2:815302/151‑1 (MQ=255)                                                                                                                                             cGTTTATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTATATGGTGACCTGCACTCGACGgatg      >  1:455022/1‑150 (MQ=255)                                                                                                                                                 tATGGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTATATGGTGACCTGCACTCGACGGATGAttt  <  1:223407/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   AAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTTTTCATCATATCTCCACTGACGAAGTATATGGTGACCTGCACTCGACGGATGATTT  >  NC_000913/3972668‑3972961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑