Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	46,040	T→G	D78E (GAT→GAG)	yaaU →	putative MFS sugar transporter; membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	46,040	0	T	G	100.0%	173.0 / NA	50	D78E (GAT→GAG)	yaaU	putative MFS sugar transporter; membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (21/29);  total (21/29)

ATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGT‑ACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGC  >  NC_000913/45895‑46186                                                                                                                                                  |                                                                                                                                                    aTGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGt                                                                                                                                                <  1:506464/151‑1 (MQ=255)   ggTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCg                                                                                                                                              >  2:759663/1‑151 (MQ=255)   ggTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCg                                                                                                                                              <  2:723423/151‑1 (MQ=255)      tATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCgg                                                                                                                                             <  1:258460/149‑1 (MQ=255)       aTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACg                                                                                                                                          <  2:350534/151‑1 (MQ=255)           aCTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGgcg                                                                                                                                       <  1:109748/150‑1 (MQ=255)              ggTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCa                                                                                                                                     <  2:271294/149‑1 (MQ=255)               gTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCaaa                                                                                                                                   >  2:340571/1‑150 (MQ=255)                tAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAAt                                                                                                                                 >  1:130638/1‑151 (MQ=255)                      ttGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTcc                                                                                                                            >  1:368402/1‑150 (MQ=255)                       tggcgtggcgCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCt                                                                                                                           <  2:504194/150‑1 (MQ=255)                           gtggcgCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTg                                                                                                                      >  2:330349/1‑151 (MQ=255)                             ggcgCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGa                                                                                                                     <  1:717978/150‑1 (MQ=255)                                    gAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCg                                                                                                             <  1:765411/151‑1 (MQ=255)                                     aGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCTCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGc                                                                                                            <  2:505864/151‑1 (MQ=255)                                        aaCTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCAt                                                                                                         <  2:205059/151‑1 (MQ=255)                                         aCTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATc                                                                                                        <  2:559821/151‑1 (MQ=255)                                                              ggACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGcgac                                                                                    <  2:427787/150‑1 (MQ=255)                                                              ggACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGcgac                                                                                    <  1:759235/150‑1 (MQ=255)                                                                  gCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGAtgt                                                                               >  1:188071/1‑151 (MQ=255)                                                                   cTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGAtgtt                                                                              >  1:510644/1‑151 (MQ=255)                                                                     gACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGAtgttt                                                                             <  1:809951/150‑1 (MQ=255)                                                                         ggATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTc                                                                        <  1:309523/151‑1 (MQ=255)                                                                                  tGCTGGGCGCGGGAACGCCCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGt                                                               >  1:475447/1‑151 (MQ=255)                                                                                    cTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCg                                                             >  1:147979/1‑151 (MQ=255)                                                                                     tGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCg                                                             >  2:559667/1‑150 (MQ=255)                                                                                        gcgcGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACt                                                         <  1:229784/151‑1 (MQ=255)                                                                                          gcgGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGt                                                       >  1:779756/1‑151 (MQ=255)                                                                                                     cGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCgg                                            <  1:316469/151‑1 (MQ=255)                                                                                                      gCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCggg                                           <  2:459552/151‑1 (MQ=255)                                                                                                          ggCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGT‑ACt                                      <  2:826214/151‑1 (MQ=255)                                                                                                           gCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGT‑ACtt                                     >  1:105464/1‑151 (MQ=255)                                                                                                           gCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGT‑ACtt                                     <  1:60689/151‑1 (MQ=255)                                                                                                                 cGTTGGCACATCGCTGTTTGGTTATATTGCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGACCTGTTGGTGATGCGGGT‑ACTTAGCgg                                <  1:153892/150‑1 (MQ=255)                                                                                                                     ggCACATCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGCTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGT‑ACTTAGCGGCCTTg                           >  1:781739/1‑151 (MQ=255)                                                                                                                       cacaTCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGT‑ACTTAGCGGCATTGTc                         >  1:418424/1‑151 (MQ=255)                                                                                                                        acaTCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGT‑ACTTAGCGGCATTGTCa                        <  1:421369/151‑1 (MQ=255)                                                                                                                         caTCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGT‑ACTTAGCGGCATTGTCAt                       >  2:678812/1‑151 (MQ=255)                                                                                                                         caTCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGT‑ACTTAGCGGCATTGTCAt                       >  2:66818/1‑151 (MQ=255)                                                                                                                          aTCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGT‑ACTTAGCGGCATTGTCATc                      <  1:330338/151‑1 (MQ=255)                                                                                                                          aTCGCTGTTTGGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGT‑ACTTAGCGGCATTGTCATc                      <  1:14834/151‑1 (MQ=255)                                                                                                                               tGTTTCGTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGCGATGCGGGT‑ACTTAGCGGCATTGTCATCGGt                   >  1:500566/1‑149 (MQ=255)                                                                                                                                    ggTTATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGT‑ACTTAGCGGCATTGTCATCGGTGCAGAt             <  1:245520/150‑1 (MQ=255)                                                                                                                                      ttATATTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGT‑ACTTAGCGGCATTGTCATCGGTGCAGATTAt          >  1:644213/1‑151 (MQ=255)                                                                                                                                       tataTTTCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGT‑ACTTAGCGGCATTGTCATCGGTGCAGATTAt          <  2:820082/150‑1 (MQ=255)                                                                                                                                           tttCCGCGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGCGGCGACGAAGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGT‑ACTTAGCGGCATTGCCATCGGTGCAGATTATCCCAt     >  1:289619/1‑151 (MQ=255)                                                                                                                                           tttCCGAGAAAGTCTGACGGCGCAAAATGCTCCTCATTGATATCATCGCCATCGGCGTGATCTCGGGGGCAACGCTGTTTGTTTCATCCCCCGCCGAACTGTTGGTGATGCGGGT‑ACTTAGCGGCCTTTTCCTCGGTGCAGATTATCCCAt     >  1:375122/1‑151 (MQ=255)                                                                                                                                             tCCGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGT‑ACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATc    <  1:616306/150‑1 (MQ=255)                                                                                                                                              ccGAGAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGT‑ACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGc  <  2:331295/151‑1 (MQ=255)                                                                                                                                               cGAGAAAGTCGGACGGCGCAAAATGTTCCTCAATGATATCATCTCCATCGGCGTGGGGTCGGTGGCGACGGTGTTTGTTTCATCCCCCCTCGAACTGTTGGTGATGCGGGTCACTTCT‑GGCAGTATCACTGGTGCAGATTATccttcct   >  1:772189/1‑144 (MQ=255)                                                                                                                                                  |                                                                                                                                                    ATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGT‑ACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGC  >  NC_000913/45895‑46186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑