Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,034,898	T→G	R97R (CGT→CGG)	hemG →	protoporphyrin oxidase, flavoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,034,898	0	T	G	100.0%	204.1 / NA	56	R97R (CGT→CGG)	hemG	protoporphyrin oxidase, flavoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (31/25);  total (31/25)

ACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGTACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTGCGCGGTCATTGCCGGGGCGCTGCGTTACCCACGTTATCGCTGGTACGACCGTTTTATGATCAAGCTGATTATGAAGATGT  >  NC_000913/4034753‑4035043                                                                                                                                                  |                                                                                                                                                  aCCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTcc                                                                                                                                              <  2:311762/151‑1 (MQ=255)  ccGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTcc                                                                                                                                              >  1:211213/1‑150 (MQ=255)  ccGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTcc                                                                                                                                              >  1:390107/1‑150 (MQ=255)          tCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCa                                                                                                                                      >  2:711357/1‑150 (MQ=255)                  gCTTCTATTCGCTATGGTCACTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTAc                                                                                                                              <  2:108290/150‑1 (MQ=255)                      cTATTCGCTATGGTCACTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTAcgcgcg                                                                                                                         >  2:167211/1‑151 (MQ=255)                        aTTCGCTATGGTCACTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTAcgcgc                                                                                                                          <  1:482799/148‑1 (MQ=255)                        aTTCGCTATGGTCACTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACgcgcgc                                                                                                                        <  2:706010/150‑1 (MQ=255)                        aTTCGCTATGGTCACTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCa                                                                                                                       <  1:105997/151‑1 (MQ=255)                           cGCTATGGTCACTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAg                                                                                                                     <  1:686263/150‑1 (MQ=255)                            gCTATGGTCACTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGtt                                                                                                                   >  2:37034/1‑151 (MQ=255)                             cTATGGTCACTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGttt                                                                                                                  >  2:426979/1‑151 (MQ=255)                               aTGGTCACTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCt                                                                                                                >  2:723030/1‑151 (MQ=255)                               aTGGTCACTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCt                                                                                                                >  2:78580/1‑151 (MQ=255)                               aTGGTCACTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCt                                                                                                                <  1:3446/151‑1 (MQ=255)                                tGGTCACTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCtg                                                                                                               >  1:747536/1‑151 (MQ=255)                                     aCTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCtgatga                                                                                                           >  2:2913/1‑150 (MQ=255)                                        aCCATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTCTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTc                                                                                                       <  1:320575/151‑1 (MQ=255)                                         ccATTCAGCGTTCCAGGAATTTGTCACAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCg                                                                                                      >  2:358071/1‑151 (MQ=255)                                         ccATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCg                                                                                                      >  1:401656/1‑151 (MQ=255)                                           aTTCAGCGTTCCAGGAATTTGTCAAAAAACATGAGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGc                                                                                                     >  1:760535/1‑150 (MQ=255)                                             tCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCaa                                                                                                   <  2:210397/150‑1 (MQ=255)                                                gCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGc                                                                                               >  2:93037/1‑151 (MQ=255)                                                  gTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGt                                                                                             >  1:490648/1‑151 (MQ=255)                                                     ccAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTcc                                                                                           <  1:431763/150‑1 (MQ=255)                                                        ggAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGAt                                                                                       <  1:541247/151‑1 (MQ=255)                                                           aTTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGc                                                                                    >  2:136769/1‑151 (MQ=255)                                                            tttttcccacaccctGCGACGCGGCTGAATTCTATTCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCt                                                                                   >  2:375516/15‑151 (MQ=255)                                                             ttGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCt                                                                                   <  1:614659/150‑1 (MQ=255)                                                                tCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTgcgc                                                                               >  1:148279/1‑151 (MQ=255)                                                                tCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTgcgc                                                                               >  1:221040/1‑151 (MQ=255)                                                                tCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTgcgc                                                                               >  2:856441/1‑151 (MQ=255)                                                                 cAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTGcgcg                                                                              >  2:40870/1‑151 (MQ=255)                                                                   aaaaaCATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTGCGCGGt                                                                            <  1:651393/151‑1 (MQ=255)                                                                           gCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTGCGCGGTCATTGCCg                                                                    <  1:256582/151‑1 (MQ=255)                                                                             gACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTGCGCGGTCATTGCCggg                                                                  <  1:656308/151‑1 (MQ=255)                                                                              aCGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTGCGCGGTCATTGCCgggg                                                                 <  2:335474/151‑1 (MQ=255)                                                                               cgcgGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTGCGCGGTCATTGCCGGGgc                                                                >  1:614134/1‑151 (MQ=255)                                                                                      gAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTGCGCGGTCATTGCCGGGGCGCTGc                                                           <  2:160465/149‑1 (MQ=255)                                                                                      gAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTGCGCGGTCATTGCCGGGGCGCTGCg                                                          >  1:201801/1‑150 (MQ=255)                                                                                          tCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTGCGCGGTCATTGCCGGGGCGCTGCGTTAcc                                                     >  1:458458/1‑151 (MQ=255)                                                                                            gATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTGCGCGGTCATTGCCGGGGCGCTGCGTTAccc                                                    >  1:619119/1‑150 (MQ=255)                                                                                              tGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTGCGCGGTCATTGCCGGGGCGCTGCGTTACCCACg                                                 >  1:574124/1‑151 (MQ=255)                                                                                               gCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTGCGCGGTCATTGCCGGGGCGCTGCGTTACCCACg                                                 <  2:624552/150‑1 (MQ=255)                                                                                                  gAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTGCGCGGTCATTGCCGGGGCGCTGCGTTACCCACGTTAt                                             >  2:541188/1‑151 (MQ=255)                                                                                                    gcgcCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTGCGCGGTCATTGCCGGGGCGCTGCGTTACCCACGTTATCg                                           <  1:122765/151‑1 (MQ=255)                                                                                                    gcgcCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTGCGCGGTCATTGCCGGGGCGCTGCGTTACCCACGTTATCg                                           <  1:648002/151‑1 (MQ=255)                                                                                                          tttACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTGCGCGGTCATTGCCGGGGCGCTGCGTTACCCACGTTATCGCTGGt                                      <  2:724154/150‑1 (MQ=255)                                                                                                           ttACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTGCGCGGTCATTGCCGGGGCGCTGCGTTACCCACGTTATCGCTGGTa                                     <  1:924881/150‑1 (MQ=255)                                                                                                            tACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTGCGCGGTCATTGCCGGGGCGCTGCGTTACCCACGTTATCGCTGGTAc                                    <  2:658826/150‑1 (MQ=255)                                                                                                               tCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTGCGCGGTCATTGCCGGGGCGCTGCGTTACCCACGTTATCGCTGGTACGAcc                                >  1:220360/1‑151 (MQ=255)                                                                                                                 cGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTGCGCGGTCATTGCCGGGGCGCTGCGTTACCCACGTTATCGCTGGTACGACCGt                              <  1:476177/151‑1 (MQ=255)                                                                                                                    gAATCTGGTGGCGCGCAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTGCGCGGTCATTGCCGGGGCGCTGCGTTACCCACGTTATCGCTGGTACGACCGttt                            <  1:845711/150‑1 (MQ=255)                                                                                                                                  gcAAACCGGAGAAGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTGCGCGGTCATTGCCGGGGCGCTGCGTTACCCACGTTATCGCTGGTACGACCGTTTTATGATCAAGCTGAt             >  2:721753/1‑151 (MQ=255)                                                                                                                                             aaGCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTGCGCGGTCATTGCCGGGGCGCTGCGTTACCCACGTTATCGCTGGTACGACCGTTTTATGATCAAGCTGATTATGAAGATGt  >  1:794124/1‑151 (MQ=255)                                                                                                                                               gCGGACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTGCGCGGTCATTGCCGGGGCGCTGCGTTACCCACGTTATCGCTGGTACGACCGTTTTATGATCAAGCTGATTATGAAGATGt  >  2:52196/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                  ACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGGAGAAGCGTACTCCACAGACCAACAGCTACGCGCGCAAGTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTGCGCGGTCATTGCCGGGGCGCTGCGTTACCCACGTTATCGCTGGTACGACCGTTTTATGATCAAGCTGATTATGAAGATGT  >  NC_000913/4034753‑4035043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑