Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,093,592	T→G	L227F (TTA→TTC)	rhaD ←	rhamnulose‑1‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,093,592	0	T	G	100.0%	254.7 / NA	67	L227F (TTA→TTC)	rhaD	rhamnulose‑1‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (33/34);  total (33/34)

TTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGC  >  NC_000913/4093448‑4093737                                                                                                                                                 |                                                                                                                                                  ttACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCg                                                                                                                                             >  1:779822/1‑151 (MQ=255) ttACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCg                                                                                                                                             >  1:28078/1‑151 (MQ=255)   aCAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGaa                                                                                                                                           <  2:781558/151‑1 (MQ=255)   aCAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGa                                                                                                                                            <  1:427678/150‑1 (MQ=255)    cagcgccagcgcACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAg                                                                                                                                          <  1:889875/151‑1 (MQ=255)    cagcgccagcgcACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAg                                                                                                                                          <  1:614910/151‑1 (MQ=255)     agcgccagcgcACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAgg                                                                                                                                         >  2:111378/1‑151 (MQ=255)        gccagcgcACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGttt                                                                                                                                      <  2:828754/151‑1 (MQ=255)           agcgcACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCa                                                                                                                                    >  2:700291/1‑150 (MQ=255)             cgcACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATcc                                                                                                                                 >  1:501949/1‑151 (MQ=255)                aCTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGc                                                                                                                              <  1:569527/151‑1 (MQ=255)                 cTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGACGGTTTCATCCAGc                                                                                                                              >  1:260368/1‑150 (MQ=255)                    gCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCg                                                                                                                          <  2:594244/151‑1 (MQ=255)                       aGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTc                                                                                                                       <  1:413952/151‑1 (MQ=255)                       aGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTc                                                                                                                       >  2:397302/1‑151 (MQ=255)                        gTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTcc                                                                                                                      <  2:39436/151‑1 (MQ=255)                               aaCGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGcc                                                                                                                <  2:782357/150‑1 (MQ=255)                                 cGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGa                                                                                                              >  1:556378/1‑150 (MQ=255)                                  gCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAg                                                                                                            <  1:862397/151‑1 (MQ=255)                                    cGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGAc                                                                                                          >  1:467255/1‑151 (MQ=255)                                     gAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACg                                                                                                         >  2:456915/1‑151 (MQ=255)                                      aaaCGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGc                                                                                                        <  2:624658/151‑1 (MQ=255)                                      aaaCGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGc                                                                                                        >  1:860008/1‑151 (MQ=255)                                               ccGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAgg                                                                                               >  1:606562/1‑151 (MQ=255)                                                 gAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGc                                                                                             >  2:4211/1‑151 (MQ=255)                                                     gcTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCcac                                                                                          >  2:149984/1‑150 (MQ=255)                                                           aaCTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCa                                                                                   <  2:389693/151‑1 (MQ=255)                                                           aaCTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCa                                                                                   <  2:311945/151‑1 (MQ=255)                                                                 tCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGa                                                                               <  1:697186/149‑1 (MQ=255)                                                                   aCGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGt                                                                           >  1:66407/1‑151 (MQ=255)                                                                   aCGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGt                                                                           <  2:76818/151‑1 (MQ=255)                                                                    cGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGtt                                                                          <  1:836267/151‑1 (MQ=255)                                                                        tGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTg                                                                      >  2:315221/1‑151 (MQ=255)                                                                        tGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTg                                                                      >  2:201776/1‑151 (MQ=255)                                                                          aTGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCa                                                                    <  1:837511/151‑1 (MQ=255)                                                                             gTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCAtct                                                                 <  1:317650/151‑1 (MQ=255)                                                                                tGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCtt                                                              >  1:56313/1‑151 (MQ=255)                                                                                tGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCtt                                                              >  2:184481/1‑151 (MQ=255)                                                                                 gTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATctct                                                               <  1:244774/149‑1 (MQ=255)                                                                                    tcttGCCGCCCACCGAATAAAACTTCACTAATTCTTGTGCTGATTTTTCTGCGGGGGCGATGAAGCCCAGGGTTTCAACCAGCCTCGGACCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTtgtg                                                           <  1:251673/147‑1 (MQ=255)                                                                                     cATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCCGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCg                                                         <  1:355650/151‑1 (MQ=255)                                                                                       tGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCgg                                                        >  1:740945/1‑150 (MQ=255)                                                                                        gccgccCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGt                                                       >  2:108732/1‑150 (MQ=255)                                                                                        gccgccCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGTc                                                      <  2:12454/151‑1 (MQ=255)                                                                                             ccATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGTCGCCTg                                                 >  1:459347/1‑151 (MQ=255)                                                                                             ccATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGTCGCCTg                                                 <  1:516327/151‑1 (MQ=255)                                                                                               aTCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGTCGCCTGGc                                               >  1:930113/1‑151 (MQ=255)                                                                                                  gAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGTCGCCTGGCCg                                             >  2:261345/1‑150 (MQ=255)                                                                                                      aaaCCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGTCGCCTGGCCGAttt                                         <  1:306143/150‑1 (MQ=255)                                                                                                         ccTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTc                                     >  2:640467/1‑151 (MQ=255)                                                                                                          cTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTcc                                    >  1:769206/1‑151 (MQ=255)                                                                                                            tCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTcc                                    <  2:863716/149‑1 (MQ=255)                                                                                                             cACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTg                                 <  1:548064/151‑1 (MQ=255)                                                                                                                  aTACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCggg                            <  2:520915/151‑1 (MQ=255)                                                                                                                  aTACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCg                              <  1:665995/149‑1 (MQ=255)                                                                                                                   tACTTGTGCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGc                           >  2:723389/1‑151 (MQ=255)                                                                                                                          gCTGATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATcc                    >  2:294087/1‑151 (MQ=255)                                                                                                                             gATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCa                   <  2:711076/149‑1 (MQ=255)                                                                                                                             gATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCAc                  <  1:341090/150‑1 (MQ=255)                                                                                                                             gATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACg                 <  1:71761/151‑1 (MQ=255)                                                                                                                             gATTTTTCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACg                 >  1:177858/1‑151 (MQ=255)                                                                                                                                 tttCTGCGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCa              <  2:260375/150‑1 (MQ=255)                                                                                                                                       cGGTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGcc       >  1:269186/1‑151 (MQ=255)                                                                                                                                        ggTGTCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGTCGCTTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCa      <  1:772652/151‑1 (MQ=255)                                                                                                                                         gtgtCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCa      >  2:456790/1‑150 (MQ=255)                                                                                                                                         gtgtCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCa      >  2:107084/1‑150 (MQ=255)                                                                                                                                            tCGATGAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGc  >  2:299734/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                  TTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGC  >  NC_000913/4093448‑4093737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑