Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,097,080	A→C	I123M (ATT→ATG)	rhaB ←	rhamnulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,097,080	0	A	C	100.0%	244.2 / NA	63	I123M (ATT→ATG)	rhaB	rhamnulokinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (36/27);  total (36/27)

CATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATAAATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAGGCCCACACGCTGACCCTGTTGGTCGAGCAGCACAAAGTCCACGCCCCAGGTATCAATCCCAATGCTAT  >  NC_000913/4096933‑4097225                                                                                                                                                    |                                                                                                                                                  cATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACat                                                                                                                                                 >  1:191837/1‑150 (MQ=255)    cTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATcg                                                                                                                                             >  1:276676/1‑151 (MQ=255)     ttGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATcgc                                                                                                                                            >  2:699724/1‑151 (MQ=255)      tGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATc                                                                                                                                              <  2:819016/148‑1 (MQ=255)        ccGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGtt                                                                                                                                         >  1:289208/1‑151 (MQ=255)               ggCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAg                                                                                                                                  <  1:466431/151‑1 (MQ=255)                  gATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAgttg                                                                                                                               <  1:277469/151‑1 (MQ=255)                     aaCTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAgttgttg                                                                                                                            >  2:8191/1‑151 (MQ=255)                     aaCTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAgttgtt                                                                                                                             <  1:284095/150‑1 (MQ=255)                     aaCTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAgttgtt                                                                                                                             <  1:593587/150‑1 (MQ=255)                      aCTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTGAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAgttgttgt                                                                                                                           >  1:539788/1‑151 (MQ=255)                               aTCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTgc                                                                                                                  >  2:192021/1‑151 (MQ=255)                               aTCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTgc                                                                                                                  <  2:208611/151‑1 (MQ=255)                               aTCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTgc                                                                                                                  <  1:719864/151‑1 (MQ=255)                               aTCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTgc                                                                                                                  <  2:609672/151‑1 (MQ=255)                                tCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTgcg                                                                                                                 >  2:811547/1‑151 (MQ=255)                                 ccGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTgcgc                                                                                                                <  1:734494/151‑1 (MQ=255)                                  cGGCATCAGCAGAGCGTGAGCAATGTGAGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATAGCGTTTGCCGAGTTGTTGTTGTGCCTg                                                                                                                   >  2:447912/1‑147 (MQ=255)                                   ggCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCa                                                                                                              <  1:34099/151‑1 (MQ=255)                                     cATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCAtt                                                                                                            <  1:277163/151‑1 (MQ=255)                                      aTCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCAt                                                                                                             >  2:904726/1‑149 (MQ=255)                                        cagcagAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAgg                                                                                                         >  2:452130/1‑151 (MQ=255)                                         agcagAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGc                                                                                                        >  2:358662/1‑151 (MQ=255)                                           cagAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGcc                                                                                                       >  1:163275/1‑150 (MQ=255)                                            agagCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCCGCGCCATTAGGCCAt                                                                                                     >  2:556404/1‑151 (MQ=255)                                              agCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTg                                                                                                   <  2:142456/151‑1 (MQ=255)                                                  tGAGCAATGTGTGGAATTAGATCAGGTTGTTGCTCAGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGATGTTGTTGTGCCTGCGCCATTAGGCCATTGATGc                                                                                               >  1:207771/1‑151 (MQ=255)                                                   gAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCg                                                                                              <  1:406194/151‑1 (MQ=255)                                                   gAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCg                                                                                              >  1:860296/1‑151 (MQ=255)                                                    aGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCgg                                                                                             >  1:472802/1‑151 (MQ=255)                                                          gtgtgGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATcg                                                                                       >  1:864795/1‑151 (MQ=255)                                                           tgtgGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACAAATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATcgc                                                                                      >  2:201406/1‑151 (MQ=255)                                                                    aGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCa                                                                              >  2:464803/1‑150 (MQ=255)                                                                          ggTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGc                                                                        >  2:100/1‑150 (MQ=255)                                                                          ggTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCa                                                                       <  1:754745/151‑1 (MQ=255)                                                                           gttgttGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAg                                                                      <  2:694304/151‑1 (MQ=255)                                                                            ttgttgCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAgg                                                                     >  2:142403/1‑151 (MQ=255)                                                                                tgCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAGGccc                                                                  <  1:910027/150‑1 (MQ=255)                                                                                  cTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAGGCCcac                                                                >  1:782459/1‑150 (MQ=255)                                                                                   tCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAGGCCCacac                                                              >  2:924459/1‑151 (MQ=255)                                                                                    ccGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAGGCCCacac                                                              <  1:84900/150‑1 (MQ=255)                                                                                    ccGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAGGCCCacac                                                              <  1:698585/150‑1 (MQ=255)                                                                                      gTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAGGCCCACACGCt                                                           >  2:877697/1‑151 (MQ=255)                                                                                         aGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAGGCCCACACGCTGAc                                                        >  1:12263/1‑151 (MQ=255)                                                                                           cgcacgcaACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAGGCCCACACGCTGAccc                                                      <  2:445899/151‑1 (MQ=255)                                                                                                  aaCTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAGGCCCACACGCTGACCCTGTTGGt                                               >  2:759018/1‑151 (MQ=255)                                                                                                   aCTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAGGCCCACACGCTGACCCTGTTGGTc                                              <  1:466366/151‑1 (MQ=255)                                                                                                          aaGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAGGCCCACACGCTGACCCTGTTGGTCGagcagc                                       <  1:840766/151‑1 (MQ=255)                                                                                                                aTTGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAGGCCCACACGCTGACCCTGTTGGTCGAGCAGCACaaa                                  <  2:354700/150‑1 (MQ=255)                                                                                                                  tGAAGGGCAGAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAGGCCCACACGCTGACCCTGTTGGTCGAGCAGCACAAAGTc                               >  1:842240/1‑151 (MQ=255)                                                                                                                           gAAACTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAGGCCCACACGCTGACCCTGTTGGTCGAGCAGCACAAAGTCCACGcccc                       >  1:45250/1‑150 (MQ=255)                                                                                                                            aaaCTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAGGCCCACACGCTGACCCTGTTGGTCGAGCAGCACAAAGTCCACGcccc                       >  1:736276/1‑149 (MQ=255)                                                                                                                             aaCTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAGGCCCACACGCTGACCCTGTTGGTCGAGCAGCACAAAGTCCACGCCCCAgg                    >  2:191752/1‑151 (MQ=255)                                                                                                                             aaCTGGATGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAGGCCCACACGCTGACCCTGTTGGTCGAGCAGCACAAAGTCCACGCCCCAgg                    <  1:548194/151‑1 (MQ=255)                                                                                                                                   aTGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAGGCCCACACGCTGACCCTGTTGGTCGAGCAGCACAAAGTCCACGCCCCAGGTATCaa              <  1:358648/151‑1 (MQ=255)                                                                                                                                   aTGCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAGGCCCACACGCTGACCCTGTTGGTCGAGCAGCACAAAGTCCACGCCCCAGGTATCa               >  2:159030/1‑150 (MQ=255)                                                                                                                                     gCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAGGCCCACACGCTGACCCTGTTGGTCGAGCAGCACAAAGTCCACGCCCCAGGTATCAAt             <  1:783440/150‑1 (MQ=255)                                                                                                                                     gCCGCTACGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAGGCCCACACGCTGACCCTGTTGGTCGAGCAGCACAAAGTCCACGCCCCAGGTATCAATc            <  1:550017/151‑1 (MQ=255)                                                                                                                                           aCGTTGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAGGCCCACACGCTGACCCTGTTGGTCGAGCAGCACAAAGTCCACGCCCCAGGTATCAATCCCAAt       <  2:296380/150‑1 (MQ=255)                                                                                                                                              ttGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAGGCCCACACGCTGACCCTGTTGGTCGAGCAGCACAAAGTCCACGCCCCAGGTATCAATCCCAATGCt    >  2:69791/1‑150 (MQ=255)                                                                                                                                              ttGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAGGCCCACACGCTGACCCTGTTGGTCGAGCAGCACAAAGTCCACGCCCCAGGTATCAATCCCAATGCt    >  1:744325/1‑150 (MQ=255)                                                                                                                                              ttGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAGGCCCACACGCTGACCCTGTTGGTCGAGCAGCACAAAGTCCACGCCCCAGGTATCAATCCCAATGCt    >  1:437153/1‑150 (MQ=255)                                                                                                                                               tGATACATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAGGCCCACACGCTGACCCTGTTGGTCGAGCAGCACAAAGTCCACGCCCCAGGTATCAATCCCAATGCTAt  >  2:64043/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                  CATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATAAATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCATTGGTGCGGCTATCGCGATAAGCAACGGGCAGGCCCACACGCTGACCCTGTTGGTCGAGCAGCACAAAGTCCACGCCCCAGGTATCAATCCCAATGCTAT  >  NC_000913/4096933‑4097225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑